Single-Cell Protein Expression Atlas of Transcription Factors

RCOR-1

WormBase description: Is an ortholog of human RCOR2 (REST corepressor 2). Is predicted to have several functions, including DNA-binding transcription factor activity; transcription corepressor activity; and transcription factor binding activity.

KI information: mNeonGreen knockin at C-terminus

sgRNA1: GCCGAGCTCAAGTCAAAACT

sgRNA2: GGACAATGAAAAGCTAATTT

5'HR: GCATGCAAAATTTTGAAAATTAGAAAAATTAACTTTTAAAAATAAAAATTGGTACAATTCCTTTTCTACCCCGAATTTTTAAAAGAATATTGAATTATTCGAATTAGATTAGAAAAGGAAATGTGCCTTGAAATTCTGATTTTTTTTCCGGAATATTCAACGAAAAAAAGTAGATTTTTTGCAGAAAATAATTTTTATCGATAACTTCAGAGAAAATTGAAAATTTTTTTTAATATCCGAAACTTTAAATACCCGAAAAATATAAATTCCAAGGAAAATTCAATTTTTCATCGAAAAATCTCCAGGAATTCAGAGAAAATTGAAAAATCTTGATTTTTTAAATGTTTTGCGGTAAAATTGACTTTTCACGTGATTAATCATGAAAAAAAAATTCAAACCTAAAAAAAAATGCTGAAAAATCACAATTTCTGGTTATATTCAGTTTTTCTTTGGAAAAGTTCATTCCTTTTTAAATTTTTTTAAATCTGAAAATTGGAAATATAAAATTTCGACTTTTCTAAAAGAACAATGTGAAATTGAAGCGCGAATCCAAAAAAATTCAATTTTTTATCAGAAAATCTGCTAAATTTCATCATTTTTTTCACAATATTTTAATTTTTTATGTAAAGTTGGCGGTTTGCTTTAAAACAATCTTTAAACTCTCATAAATTGGGAAAAAAAGTTGGAAAAATTAATTTTATGTTGAAAATCGATTTGTTGCAATAAAATCTGAAGATAAATTGAATATTCTCGAAAAATTTCGCATTTTCCAAGTCTTATGATTAAGATTATTATGATTATGATTAAGCAAAAACATCCGAAATTCCTTCAGGTTTCCACTGGTACAAGGACAATTTCGAGTTGATCGCCGAGTTGATGGCCACAAAAACTGTGGAACAAATCAAAAAGTTCTATATGGACAATGAAAAACTAATTTTAGAGGTACTCACCATTTTTTATTCGGAAATAAAAAAAAACAATATTTTCAGTCAATCGACACGTATCGCGCCGAACTTAAGTCGAAGCTCGGCAAAGGTACC

3'HR: CTGCAGTTGGATCCGCTGTGTTTTTTTTAAATTTTCAATTTAACTTAATAGGTGGCCCAAAAAAAATTTCGGCCTTGAACCCCTTTTTTCTCGTTGGCTTAAAAAGTTCTTCTCGGCCACGTCGTCACAAGCTTTGTATTCACAAGGATCTCATCGTATTACATTCCACATATTTTTCCCGCCACGTGGCATAATACTCATGTTCTCATTTCTATATATTGACTTTTGAACCTTTGTCGGGCATAATTTGAAAATATTGTTTTTAGCCCATTTTTTGCATATTTTTTTGTACCCCATCCAAATGTCCGCCCCCTTCTCCCCATTTAACCCATATTTTCCGACACAAATGCTTTAATCGCTCTCATATATTCATTTTTTGTTAACGAATTTGAATCATGATTATCGTATCCTGGATAAACGAAAAATTTTCAAAATTGTTCAGAAAAAAAAACGGGATACAATACACTTTTCAAATTGGTTTAACTGTACAGTCTGGAGAAGGTGGAGTAGCTTGGATGGGAATTTTACTTTAAAATGTGCTATCTAATCCTAAATGCCGAAATACCACAAAAGAAAAACGAAAAAATCCATTAAGTTTTAAAAAAATCGGTCGATGCTGGCTAATTTTCAATTTTGACTATTCTACCCATTTGAATTACCGCGCGTTTGTTCGCTCCGCACTCCGTAGATTTCCATCCGCGCTATTTTTTTACTTCCGACGATTTTAATTTTAAAAAAACCTTTAAACGACTAAAACGTTTCTGAAAATTTTTTGAACGAGCTGAAAATCGATAATTACTTAAGTATTGATTTTCTCGTCGAATTTATTTTGAAAAATGATTTTCCGCGCCGAATTTAGCCTTTGCGCATTTCTCGTGGCTCTCTCCGCGTGCTGACTAGT

KI verification primers: RCOR-1-CKIF: 5'-CCTGAGGAAAAGAAGAATGCTATTAG-3' RCOR-1-CKIR: 5'-ATAAATTCAGCATGTTGGCAGG-3'

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Strain name: SYS667

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; rcor-1 (devKi232 [rcor-1::mNeonGreen]) I

Expression pattern: Ubiquitous

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): NA

Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: AHR-1, BCL-11, BED-3, CEH-14, CEH-18, CEH-19, CEH-2, CEH-31, CEH-34, CEH-39, CHE-1, CND-1, COG-1, DMD-4, ELT-2, ETS-4, ETS-7, FAX-1, FKH-6, HLH-1, HLH-15, HLH-8, LIM-6, LIN-13, LSY-2, MEP-1, MLS-2, NFYA-1, PAG-3, PHA-2, PHA-4, PQM-1, REF-2, RNT-1, SPTF-1, SYD-9, TBX-2, TBX-7, UNC-3, UNC-42, UNC-86, ALY-2, BLMP-1, C16A3.4, CEH-8, CEH-82, CEH-90, DPL-1, DSC-1, EFL-1, EGL-13, ELT-3, FKH-10, FKH-4, FKH-8, FOS-1, IRX-1, MDL-1, MEF-2, NHR-85, PAX-3, SKN-1, SOX-4, SWSN-7, UNC-130, UNC-30, VAB-15, ZAG-1, ZTF-11

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data