CEH-31

WormBase ID: WBGene00000452

WormBase description: Is an ortholog of human BARHL1 (BarH like homeobox 1) and BARHL2 (BarH like homeobox 2). Is predicted to have DNA-binding transcription activator activity, RNA polymerase II-specific and RNA polymerase II regulatory region sequence-specific DNA binding activity. Is expressed in head neurons and pharynx.

KI information: mNeonGreen knockin at N-terminus

sgRNA1: GTGGGGTCTAGAAAGAATGC

sgRNA2: TAAGTTTATCTTCGCGCCAC

5'HR: GCATGCCACAAGTACCAAAAGATGTTACCAGTTAATCTGAACTGTACGTGTGAAGCAAGTTTAAGATCACAGCCAGGAAAATCTAAATGCAAAATGAACTATCAAAAGTAGTCACTGTTACTGAACTTTTCAGATAGTGTTTTCCATTGTGCTGATTTCAAATCAGTGATGTATCAAATATTAAAACACACACAATGTACCTATTAGACCTCATCATCTTTTAGTCAGAAAGCTGAAACATCATTCGGGCTGAAACTCATTTTTAGCTTTAGTCGCTAGTTTTTGCACGCCGAAGCAGCATGCTATTTTTGATTTTTTCTGCGGTTTTACACTAGCATAGCTAGTTATATTGAATTCTATTCAGTGCTTTTTTATCTGAAAATCATTGCTCGTTTGGATCATGTTGCATTGCGTTGTTACTCTGCTTTTAGGAAAAGCCTAGTGTGCATAGTTTGCATTCGGCAGTTTCGGGATTTGTCTGAATTGTTTTCTTCTCACCTGAATGCCTCATTTCCGAAACTTTTGCTTTTCGTCTTTTCCTCGAAGCTGCTCGTTTTCAGCTTCATCGTCCCCCATTATCACACTTGATTGGCTTCAAAAAGGTGGATTGCACGGACCACACTTCTCAATACAAAACCGCGTGGTGTCATCCATTCTATTCCCTTCTTCGACACGCCCCGCCTTCCACTTCGCATGGGCGTGACTAGACCAATCAACGAGTTTGCGACGTTTAGTGACTGCAGGCAACAGGACCCTTCCAACCCACTCATTCACACTAAACTACTGTCTAAAAGTCATTCAATTTGACTATTACAAAACTTATTGTATTGTTACATCATCTGATAAGGTTATCATAACAGGTACC

3'HR: CTGCAGATGTCAATGTTGGATCTCCGTCAGTTTCTGATTCCTGCTTTTTTCCTTGATCCAACCAACCAACTTGCTGCTCAGAGTTCTTCTGGTGGCGCGAAGATAAACTTAAACAGCTCTTTTCGCATTTCTGATCTGCTTGATCCAACTTCACACTCAACCAACGAACCAGGTAATCCATTTTGCCACCATTTTCCTAAAGTTATTGCATTTATTGTATGTTCGTTCAACCATCTCTAACACTTTAATCTACATAGACTTCCATGAGAATGTTGAATGTACAACTAACCTGAAAAATGATATTTAACGGATTAACGCTCCAGTAGTCACATGGACGGTAATCTGTAATAGTAATAGGTCAACCAATTATGATCCTTTGTTCTCCTCTTACCTATACAAACAGTTTTCTTTATTTATTTGTTATTGTAGATCCATCTCCTACCTCCATTAATTCCTCGGAATCGCCATCATCCCCTAGAATTGGGAGCCCGAATACCGAAAGATCTGTGAACGAGAGTCCGACATGCGTGGGATCAAAAAAGGCTCGGAAGGCGAGAACTATCTTCACCGACAAACAACTTCAAGAGCTAGAAAACACCTTTGAGAAGCAGAAGTACTTGTCTGTTCAAGATAGGATGGAGCTGGCTCATCGAATGGGGCTAACAGATACTCAAGTAAAGACATGGTATCAAAATAGAAGGTTGGTCGGCTATATTCCTTAATTAGGCAAACTTTTTTTCTTTTCTCGAAAAACAACAACGTGTGGCTCTTTGCGCCAGCAGTTGGGCGAGAATTGAGTCTAAACACGCCACTTTGTGTTAATTGTAGTGCATTAGCGAGAAGCTTCACCCCCATCGAAATTTATCTTAATCAAAGACTAGT

KI verification primers: CEH-31-NKIF: 5'-AGTTTAAGATCACAGCCAGGAA-3' CEH-31-NKIR: 5'-TTGAAGAAGTTGGGACGA-3'

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Strain name: SYS867

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; ceh-31 (devKi250 [mNeonGreen::ceh-31]) X

Expression pattern: Lineage-specific, Neuronal-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): BED-3, CEH-30, CEH-8, PAG-3, UNC-86, VAB-3, LIN-32, SEM-2, MLS-2, EGL-44, SOX-2, CES-2, CEH-32, HLH-30, ALR-1, ATF-7, CEH-43, EFL-3, ELT-6, HLH-2, CRH-2, ZIP-8, ZTF-11


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: UNC-86, VAB-3, MLS-2, SOX-2, CES-2, HLH-30, CEH-43, HLH-2, CRH-2, ZTF-11, ALY-1, ARID-1, AST-1, ATFS-1, ATHP-1, BCL-11, BLMP-1, CAMT-1, CEBP-1, CEH-20, CEH-38, CEH-39, CEH-44, CEH-48, CEH-88, CFI-1, DAF-12, DAF-19, DAF-3, DMD-5, EFL-1, EGL-18, EGL-46, EGRH-1, EOR-1, ETS-5, FAX-1, FOS-1, GMEB-1, HBL-1, HLH-11, HLH-14, K09A11.1, LAG-1, LIM-7, LIN-13, LIN-15B, MDL-1, MEF-2, MEP-1, MML-1, NFI-1, NFYA-1, NHR-102, NHR-23, NHR-25, NHR-49, NHR-76, POP-1, RCOR-1, SDZ-38, SEL-8, SEM-4, SKN-1, SOX-4, SWSN-7, SYD-9, TAG-68, TBX-2, UNC-3, UNC-37, UNC-42, UNC-62, Y55F3AM.14, Y73E7A.1, ZAG-1, ZFH-2, ZTF-30, ZTF-6

Targeted by: REF-2

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: UNC-86, VAB-3, MLS-2, SOX-2, CES-2, HLH-30, CEH-43, HLH-2, CRH-2, ZTF-11, ALY-1, ARID-1, AST-1, ATFS-1, ATHP-1, BLMP-1, CAMT-1, CEBP-1, CEH-20, CEH-39, CEH-5, CEH-88, CFI-1, DAF-19, DAF-3, DMD-5, EFL-1, EOR-1, ETS-5, FAX-1, GMEB-1, HBL-1, K10B3.5, LAG-1, LIN-13, LIN-15B, MDL-1, MEF-2, MEP-1, MML-1, NFI-1, NFYA-1, NHR-23, NHR-25, NHR-49, NHR-76, POP-1, RCOR-1, SDZ-38, SEM-4, SKN-1, SVH-5, SWSN-7, SYD-9, TBX-2, UNC-37, UNC-42, UNC-62, Y73E7A.1, ZAG-1, ZFH-2, ZTF-30, ZTF-6

Targeted by: REF-2