HLH-14

WormBase ID: WBGene00001958

WormBase description: Is an ortholog of human ASCL1 (achaete-scute family bHLH transcription factor 1) and ASCL2 (achaete-scute family bHLH transcription factor 2). Is predicted to contribute to DNA-binding transcription factor activity. Is involved in several processes, including generation of neurons; oviposition; and positive regulation of growth rate. Localizes to nucleus. Is expressed in several structures, including ABplapppaap; ABplapppapa; ABprapppaap; ABprapppapa; and neurons. Human ortholog(s) of this gene are implicated in congenital central hypoventilation syndrome.

KI information: mNeonGreen knockin at N-terminus

sgRNA1: TCTGGCAACTTGATTCTTCT

sgRNA2: AGAACGCAAGCGAGTTCATC

5'HR: GCATGCTGAACTCTCACAGCTGTAAGGTAAGAGGTATGAATATATCTTAATAAATATTTTGACATTTTGACACTAATAGTGCGAATACGGTATGTGTAGAACTAAATTTACTAAAAATATTCAAACTAAAAATTATAGTTTTCAAACTTTTATATTCCTAACTGTAATTCTCGTTCGTTATGATATAATTTGGATTTTCACCAATTTCAAATATTTTTTCAACGACAACGAATTGTCTTTATCTTTCTGGAATACAAATCTAATTTCCGAATTTTGAAATGTACATCTTTGGTGGCAACTTGGCGTTTTATAGAGTTCAAAATAATCTTTAACAATTCGTTTGAGCGTTGAGCATTTATAAGTCAGGGCAATATTTCAATTGGTCCGTATTGGTGCGAAATTATTTGAAAGAGCCAGATTGGGTGAAAGTACGGTAACTACAAAAAAATCTGGAGTTACAAGAAAAATTATTTAATTGGCCACCTCACGAAAACTTCTAAAATGATAGCATGAAATGCCAATCAGAAAAACAAGATTTTTTTAAGTTTTAGAGAGGGATCTCATATGTGCTCGTACAAGCACTGGTCATACCTCCCACATTTTGGATCGGTAGAAGTTTTTGTAACTTTTAACTCTTCAAAAGTTGATTTACAGGGTCTAAATTTATAAAACTTTTTTGTTACATTTTAATTTGAGAATATGGGTCTGAGCTCAGATTTTCAGATTTTCCAGATTTGTACCTCCTCCCAATCCTTTGACCTCGAACTCTTCAACTCCTCCCTCTATAATTTGGTGCCAATACGGTTTGTGCCAATATCCACCCTCCTAGTCCCGCCCATCAGATTGTCCCGTCCCGCCTTTCCTCTCACCATTAGCCTTATTCATTCAGTTCACCCACACTCACTCCTAGCCACCACCGTCTTCCCTCTCTCTTTTTATTTTCCCGCCCAACAAAAAAAGGAAGCAGGTACC

3'HR: CTGCAGATGGCTAAGAAGAACCAAGTTGCCAGAAATGAGAGAGAGCGTAAGCGCGTCCACCAGGTATGTACATTTATATTGAATCAATATTTTTGAGTACTAATTTTTTTCTAACTTATTTTGTCCCTCAATGTACATTTCTTAAATAAAACATTTTCCAGGTAAACCATGGTTTCGACGTGCTCCGCAACCGTCTTCAACCAAAGAATCATACCAAGAAATGGAGCAAAGCAGACACCCTTCGTGAAGCTGTCAAGTATATTCAACAACTTCAGGTTCTTCTCAATCAAGATCCACAGCAACCATCAGGTAAGATAAGTCTTCATGTGCTAAAAGAATTTTTGAATACATATAGAGCAAAAATATAATTATTGTAGTGTAACATCATAATAAGCAAAACAAATTAAAAGCTAGCAATCATGTGTACTTCTATGTTCTAGACTGTTCTAGACTGTAATTCTGTAAAATTCGATATGCAATTTAATTAGCATTTTTGTACGAGCTGTATCTAGAATAATTAAAAGTTTGCTTTAATTTTTTCCTCACAAGTAGTATTCTTCCAATTTTCTATTTTAGTTAATTCTCAAAATTTAAAATTTGATTTTACAATTCTCAATTAATTTTTTTCAGTTTCTTCTTCAACTCCCGACTACACGATGAACAACAGCAACAACTTCAACAATTATGCCGTAAAAGAAGAATTCTCGATGTATTTGCCTCAAAACTATTGCCCACAAAATCAAATGTCAGTACCTCATGGAGATGTTTCCCATAACTTCAATTCTCCAACATCATCAGTTTCCTCATCTTCTTACTCACCAACTCAAATGTGTTATCCTCCAGTTTCATATTCCAATTATCCACACCATTAATTTGGAAAATCGATATTTTAGTATTCCCTTCTTATCATTCAAAGATCTGGTGCATTTACGGGTCTCCATACTGTTTTTGTTTTTAATACTAGT

KI verification primers: HLH-14-NKIF: 5'-ATGGGGGGATGATGCTAGAATC-3' HLH-14-NKIR: 5'-GCGATTGACACGGAATTAGCTAGT-3'

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Strain name: SYS461

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; hlh-14 (devKi119 [mNeonGreen::hlh-14]) II

Expression pattern: Lineage-specific, Neuronal-specific, Transient-expression

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): UNC-3, UNC-86, LIN-11, NOB-1, TBX-2, PHP-3, HLH-2, EGL-44, ELT-1, ZTF-11, FKH-2, CEH-43


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: ELT-1, CEH-31, ETS-7, UNC-62, ZIP-8, UNC-86, ZTF-11, BLMP-1

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: NA