CEH-43

WormBase ID: WBGene00000463

WormBase description: Is an ortholog of human DLX1 (distal-less homeobox 1) and DLX6 (distal-less homeobox 6). Is predicted to have DNA-binding transcription factor activity and RNA polymerase II proximal promoter sequence-specific DNA binding activity. Is involved in embryo development. Is expressed in several structures, including CAN; anterior hypodermis; ganglia; neuronal sheath cell; and somatic nervous system. Human ortholog(s) of this gene are implicated in amelogenesis imperfecta type 4; orofacial cleft 15; and split hand-foot malformation 1 with sensorineural hearing loss.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-GAAACAGAAGGGTGGAAGTTCG-3'

Strain name: SYS90

Cross: OH10447 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; otIs339 (ceh-43(fosmid)::GFP + ttx-3::dsRed + rol-6)

Expression pattern: Lineage-specific, Transient-expression

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): CEH-10, CEH-31, CEH-8, EGL-13, LIN-22, PAG-3, RNT-1, LIN-32, DAF-19, SPTF-1, CEH-32, MBF-1, Y56A3A.28, CEH-41, TAB-1, ELT-1, CEH-5, CEH-30, NHR-25, BLMP-1, NHR-23, LIN-11, VAB-3, UNC-39, NOB-1, HLH-30, LIN-26, HLH-14, TBX-38, GMEB-1, HAM-2, ETS-7


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: DAF-19, ELT-1, VAB-3, AST-1, CES-2, DMD-5, DPFF-1, EGL-18, EGL-46, ETS-5, K09A11.1, LIM-7, MDL-1, MLS-2, NHR-102, NHR-6, POP-1, SPR-4, UNC-130, VAB-7, Y73E7A.1, ZAG-1, ZFH-2, ZTF-6

Targeted by: CEH-31, LIN-32, SPTF-1, ELT-1, HLH-30, HAM-2, ETS-7, ALY-1, BCL-11, CEH-83, CND-1, FKH-6, HMG-4, LSY-2, MLS-2, NFYA-1, PROS-1, REF-2, TBX-2, UNC-55, UNC-62, ZIP-8, CEH-8, EGL-13, RNT-1, BLMP-1, CEH-82, EFL-1, ELT-2, FKH-8, MEP-1, NHR-85, PAX-3, SWSN-7, UNC-86

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: DAF-19, ELT-1, CES-2, DMD-5, DPFF-1, ELT-6, ETS-5, MDL-1, MLS-2, POP-1, Y73E7A.1

Targeted by: CEH-31, SPTF-1, ELT-1, HAM-2, CEH-83, FKH-6, HMG-4, LSY-2, MLS-2, NFYA-1, PROS-1, TBX-2, UNC-55, UNC-62, RNT-1, EFL-1, ELT-2, PAX-3