WormBase ID: WBGene00001253
WormBase description: Is an ortholog of several human genes including GATA1 (GATA binding protein 1); GATA3 (GATA binding protein 3); and ZGLP1 (zinc finger GATA like protein 1). Exhibits RNA polymerase II regulatory region sequence-specific DNA binding activity. Localizes to nucleus. Is expressed in several structures, including ABplaapppa; ABplaapppp; ABprpppppaa; P9/10L; and body wall muscle cell from MS lineage. Human ortholog(s) of this gene are implicated in several diseases, including Down syndrome; hypoparathyroidism-deafness-renal disease syndrome; and trichorhinophalangeal syndrome type I.
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KI information: mNeonGreen knockin at N-terminus
sgRNA1: TGAGATGGTGAGCCGGGAGG
sgRNA2: TTTACAGATGACGTCGTCGA
5'HR: GCATGCCCCACCTTAACAACTAATTCATTTTCCAATTTCCAGGTTCGCCGACCAATAGAAATGCGCAAAAACCACATCCAACAACGCTACCGCCGAAAGAACAAGGAAAAAGAGTCGTCGGCGGCAACACAGATCTTCAACCAACTCCTCACACAAATGCCGACGATGGCGACCGGCGGAGTGAGCACTGATGGAGCCATCAACACATTCAATTTACTCGAGCAAATCTCGCAATTCACGCAGGCTCAAGAGCTCATGAATAGCAGTGCCACATTTTAATTATCATTATTATTTTTTCCCCCTTCACATCCTTTTTATGCACAATACCTTTATGTAATTTTTCTTATTTATTGAAATTTGAAATTTGAAATTCTCCATAATGTATTATTGATTAATAAGCAATCCTTCATCATTCCATTACCCCTTCAGAAAAATACAGTATTAAATAATTTTCCGCCAATTTTCTAATAATAATTGTGCCCCATTTCCCCCAACTTCACCGCGTGTTTCCGACTGTGAGCTTTACCTCCTCCTCCGACAACTTATAATAATAGTTAGGCTCCGCCCCATTCTGCAGGAGGCCCCTCCCCCAATTCTCTGTGCATGGTTGTGTTATATTCACCTCCTCCCACCACTTTGTCATTTCACTTGAATCATCATTTTTGCATTTTCATATTTATTTTGAAAAAATCTCATCTCACTCTCTCTCTCTCTCCTTATCTCTTGTGGTTTTTGACGGATAAACGTATGTATCTCATTCTATCACTTTTAGCTTTTAACTTCGTCCTCTCGCTCTCTCGCACTCTCCCCTCGCTCCACAGTCTCTCTCCGTCTCTCTCTCTCTCTCGCTCTCCACTTCTTCTTCTTCTAAAACAACGTCACACGTGATTTACAGGGTACC
3'HR: CTGCAGATGACGTCGTCGAAGGAGGAGATGCCGGATGAGATGGTGAGCCGAGAGGTTGAGGAGGAGATGGAGGATCGGGTTGAGGAGACGGCGACTACAGCAGCAGCGTGCACCGGATGCGCTCAGCTTCACAAGGATATTCGACAGGTGCATATTATTTTGTTTAGCAGTAGGCTCACGCGCCTGCCTACTCTCGACATTTGGCTCCAGCTTCTTGCCCAAGTTTGGCCTAAAAAATATTAACTTGGATAAGAAGCTGGCTAAACTTGGGCAAAAGTTGGGCAAGACTTTGGCAAAACTAAAATCTGGTCAGACTTCTACCCAAGTATAACTAAAAAAAAGAAAATTTTGGACCAAAGTTTGGTAAGAGTTGGGGGAAACTTTGGTGCAGCCTGAATCCAAGTTTTGCCCAAGTTTTGCCCAATTGCCAACTAGAGCCAAATGCCGATAGGCAGGCATACATGAAGTAATTCTCGGATTACCAGAAACATTAAATTATTTTTCATTTCATTGAGAATTTCTGAAATTCTAGGAATTTGAATCGCCGCGCTGAAAGCTCCGGTCTATTCGTTTTCTTACAGGATGTTTCAAAAATGATGTCGAAAATTGACGAAGTGTGCGGAAGGCTTGAAGCTCTTATCAGTGAAAAGGAAAAAGTCCTGCTTGAGCAAATGTCCGAGAGCGGAAGCGAGGAGAAGGTGAGATTTTTGACAACTTTTAGCGGCCATGTTGCAAAAGTTCTGCGGGAAGGAGCTGGTGGAACTTTAGAAATAAGCCAAAATGGGCTGAAACTGAGCTTATTTCGCAGCATTTTCCCGCCGCCTGACAATTTTCGAGAAAGAAACTGAATCTGTTTTAAAGTTATTTTCTCAGTTTTTCGCGATTCTGAGCCATTCCCAAGAAACTTGTTTATTTTTGCATCCACTAGT
KI verification primers: ELT-6-NKIF: 5'-TTAGAAGCTCCTAAATGCCTAAGGA-3' ELT-6-NKIR: 5'-GTCACATTACTTGAAGAAACAAAGAAA-3' Download
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Strain name: SYS631 KI background: JIM113 Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; elt-6 (devKi188 [mNeonGreen::elt-6]) IV
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Expression pattern: Lineage-specific, Transient-expression
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Lineage expression pattern
350 stage
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600 stage
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3D expression pattern  4-cells  8-cells  15-cells  28-cells  ~50-cells  ~100-cells  ~200-cells  ~350-cells  ~500-cells  ~600-cells  600-cells  bean-stage |
Inferred TF relationships
Based on expression similarity (strong, medium, weak): CEH-10, UNC-42, CEH-8, RNT-1, PAG-3, TTX-3, CEH-31, CEH-48, CEH-30, SEM-2, MBF-1, EGL-46, LIN-11, DAF-19, ZAG-1, CEH-5, SOX-2, UNC-3, HLH-30, PROS-1, CEH-32, NGN-1, VAB-3, CND-1, CEH-6, CEH-16, LIN-32, Y73E7A.1, TAB-1, UNC-39, NHR-67, MLS-2
Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: MLS-2, CEH-36, CEH-83, DVE-1, HMG-4, REF-2 |
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 Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: CND-1, CEH-43, CEH-83, HMG-4, REF-2
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