CND-1

WormBase ID: WBGene00000561

WormBase description: Is an ortholog of human NEUROD1 (neuronal differentiation 1); NEUROD4 (neuronal differentiation 4); and NEUROD6 (neuronal differentiation 6). Is predicted to have DNA-binding transcription factor activity. Is involved in axonal fasciculation. Localizes to nucleus. Is expressed in several structures, including ABalpapaa; ABalppapa; ABplaapaa; ABplppaap; and neuroblasts. Human ortholog(s) of this gene are implicated in maturity-onset diabetes of the young type 6; persistent hyperplastic primary vitreous; and type 2 diabetes mellitus.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-CTAGTTGTTGAATTTTCGGAGTCCT-3'

Strain name: SYS393

Cross: OP349 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs349 (cnd-1::TY1::EGFP::3xFLAG + unc-119(+))

Expression pattern: Lineage-specific, Neuronal-specific, Transient-expression

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): UNC-3, UNC-30, VAB-3, EGL-46, ZAG-1, CEH-48, CEH-6, CEH-13, SPTF-1, SOX-2, HLH-2, FKH-2, CEH-36, ZTF-11, ATF-7, UNC-62, ELT-6, CEH-32, EFL-3


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: UNC-3, UNC-30, VAB-3, EGL-46, ZAG-1, CEH-48, CEH-6, CEH-13, SOX-2, HLH-2, ZTF-11, ATF-7, UNC-62, EFL-3, ALY-1, AST-1, CEH-20, CEH-38, CEH-39, CEH-40, CEH-43, CEH-44, CES-2, CRH-2, DAF-19, DMD-5, DPFF-1, DUXL-1, EGL-18, EGL-44, EGRH-1, ETS-5, GMEB-2, HBL-1, K09A11.1, LAG-1, LIM-4, LIM-7, LIN-13, LSY-2, MEP-1, NHR-102, NHR-23, POP-1, PROS-1, RCOR-1, SEL-8, SWSN-7, TLP-1, UNC-37, Y75B8A.6, ZFH-2, ZIP-8, ZTF-6

Targeted by: CEH-36, ELT-1, UNC-42, ZIP-8, DSC-1

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: UNC-30, VAB-3, EGL-46, HLH-2, ZTF-11, UNC-62, ELT-6, EFL-3, ALY-1, CEH-20, CEH-39, CEH-40, CES-2, CRH-2, DAF-19, DMD-5, DPFF-1, DUXL-1, EGL-44, ETS-5, GMEB-2, LAG-1, LIN-13, LSY-2, MEP-1, NHR-23, POP-1, PROS-1, RCOR-1, SWSN-7, TLP-1, UNC-37, ZFH-2, ZIP-8, ZTF-6

Targeted by: ELT-1, UNC-42, ZIP-8