CEH-44

WormBase ID: WBGene00000464

WormBase description: Is an ortholog of human CUX2 (cut like homeobox 2). Is predicted to have RNA polymerase II regulatory region sequence-specific DNA binding activity. Is expressed in dorsal nerve cord; head muscle; and ventral nerve cord.

KI information: mNeonGreen knockin at N-terminus

sgRNA1: GAGCATGGGAATCTGTAGAT

sgRNA2: ATTTCTTATTTCAGACCTTA CEH-44-NKIsg3: GGAAATCGTCTCAAGAGCAT

5'HR: GCATGCTGCGAAAAAATCAATATTTTAGCTGAATTCTTCCACTGTTTTTCTCTCAATTTTTGTATGAACTTTTCAGAAAAGTTCCCAGAAACTCCTGAAACTTGAAAAATTCTAACGTTTTTGCATATTTTCCTGAAAAAAAAACCACTTTTTTGTTAAATTTTTCGCTTTTTTGACTATTTTAAAGCTTTTTAAAACTAAAAAGTCACGAAATTCACCTTTTTTCAGTCAAAAAAGCCTTGTAAAACTGGTAAAATTTGTATTTTTCGGAATAATTTCAAATTTCGAGCCAAAATATCACTATTTAAACCTTAATTTCAGACATTTTTTAGCGACAAATTCAAATTTCTATCCAAAAAATATTGTATTTTAAGTTAAATTTTGATAGATTTTGCGGAAAAAATCCGAATTTCAAGTCAAAAAAATCAATTTTTTCAGTTTTAGGCATCATTCCCACCTGAATTTCACGAAAATCTCACTAAAAACTCATTTAAAATCCCATTTTTCGCGGCAAATTCAATTTTCCCCAATTTCAGAGGTGAAGCTCCGTGACTACAAGGCAGAAGCAGCTAAAGTGCTCAATGAGCTTCCAGCCGACTATCATCTTCGCAAAGACTACCGTAAATACAACGACTGGCGCTACAATATTACTGAAAGCTAGATTTCTCTGAAAAATTGGCCGTTTTTCTGCAAATACCCTTCGTTTTCCCGTAGAGTTCTCTCCAAATTCTCCTATTTTTTTCTTGTTTTTTCCCGTTTTTTTTTCCAAAAATTTAAATTTTTTCAGCGAATAAAATGTAGATTTTCTTTAATTTTTTTTTTTCACTTTTTCAACCTTTCTGGTTTTAATCCGTGTGCTATTTCTAACAAATTGTGTGATTTTTTGCTGTGATTAAATAATTCGATTAATTTCTTATTTCAGACCTTGGTACC

3'HR: CTGCAGATGGAAATCGTTTCGAGGGCCTGGGAGTCCGTGGATTGGGACCGAATTCAAGTTTTTTGGAAAAAAAAATTATTTAAAAAATCTGAAATTTCAGACAAGAGTGGAGGCTGAAGTGACAGCACTTGGACAACGACAAGATGACAGTGAAATTCGTAAAACACGATTGGTTGAGGAGAGTAATGCATATAGAGGCAGAACTAATAAGGTAAAGAATTGGAGAAAAATTGGCCTGAAATCCGGTGAAAATCGAATCAAAATATGGGAAAATTGCCGAAAATCCAGCAAAAATAGAGAGAAATTATGGAAAAAATGGCTGAAAATCCGGCAAAAATGGAAAGAAATTATGGAAAAAAAATTGGCTAAAATCCAGAGAAAATCCGGATAAATTGGCTGAAAATTCGGCAAAAATGCTGAGAATTTATGGAAAAATTAGCTAAAAATCTCAGCGAAAATTACTCGAAATTTTAGGAAAAAAAATGATTGAAAATCCTGCAACATTGGGGAGAAAATATATAGAAATTTGCTAAAAAACAAGAAAAAAAATGAGGAGAACTTTTGGAACACTGGGCTGAAAATTCAGAGAAAACGAGTATAAATTCTTGAAAACTGAGCTAACAATCCTGCATAATTTGAATAAAAATTCAGCGAAATTAATGAAAAATGGACCAAGCTCTGTCGATATTTATAAGTGAGGATTTTTTCCACCGATTTTTAAATACAACTTTTCTTGAAAAAAATGCAAAAATGTGCTCGGAATACTCATTTTTCAGTAATTTTCAGTTATTTTGCTTTAGAAAATAATGGTGCAAATTTTCTTTAAAAAATCGATATCTCTGGAATTTGGATCTGTGTAATTTATAAATAAGTAGTTTTTTGGAATTACTAGAAAACTTTTGGAAATGCTACAAAAAATGTAGAATTAGTACATAAAAAATGTCAATTTTTTTGAACTTTGACTAGT

KI verification primers: CEH-44-NKIF: 5'-CCATGACGACCAGAAGGAGCT-3' CEH-44-NKIR: 5'-ATGGTATGACACTTGGCGGGA-3'

Download

Strain name: SYS672

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; ceh-44 (devKi209 [mNeonGreen::ceh-44]) III

Expression pattern: Non-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): CEH-10, SKN-1


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: CEH-14, CEH-18, CEH-19, CEH-2, CEH-31, CEH-34, CEH-36, CEH-39, CEH-48, CND-1, COG-1, ETS-7, HLH-30, LIN-13, MEP-1, MLS-2, NFYA-1, REF-2, SEM-2, SPTF-1, SYD-9, TBX-2, TBX-7, UNC-42, UNC-55, UNC-62, ZTF-11, CEH-82, DPL-1, EFL-1, EGL-13, FKH-10, FKH-8, LSY-2, MDL-1, SWSN-7, UNC-86

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: CEH-18, ETS-7, SYD-9, TBX-2