WormBase ID: WBGene00001182
WormBase description: Is an ortholog of human SOX5 (SRY-box transcription factor 5) and SOX6 (SRY-box transcription factor 6). Is predicted to have DNA-binding transcription factor activity, RNA polymerase II-specific. Is involved in several processes, including cell fate specification; generation of neurons; and vulval development. Localizes to nucleus. Is expressed in several structures, including developing hermaphrodite gonad; egg-laying apparatus; intestinal cell; neurons; and uterine pi cell. Human ortholog(s) of this gene are implicated in several diseases, including 46 XY gonadal dysgenesis; PCWH syndrome; and Waardenburg's syndrome (multiple).
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KI information: mNeonGreen knockin at C-terminus
sgRNA1: TTTGTAGGAGATGTGACTGG
sgRNA2: TCAGGATCTACAAATGGACT
5'HR: GGATCCTACCCTTTACAGGCGGTTTGAATTATGAAGCTATACTGAACAATCCCGTCTTAAATGCTACTATTGCTGGACATTTGCCAAATCCTCTTGCATCGAACATTTCCTTGCTTCAAAAGAGTATTAGCGCAAAACTTGCAGCCGCTGGAAACATGCAAACAGTCGAAAAAGTGGAGACACCTTTAAATCTTTCAAAAGACACCCCATCTCCAACTGCTATTCCACAATCTCCACTATCTGGATTTCGGCTACCATACTCACTTGGGACAAATTATGGAAGCGATGGTATTATTCTAAAGCTTTCACTATAAAATTAAAAGTTTCATATTTTCAGGTCAATTATTTAACAATTGCTCACCAAATTCCAGTGGTAAGAGCACGCCTGGTAACACATCAGTAACTAGTGAAGTAGCAACTCCACGACCACAGGCCAAATCACCAAACCATATCAAAAGACCAATGAACGCATTCATGGTATGGGCTCGTGACGAGAGACGAAAAATTTTGAAAGCGTATCCGGATATGCACAACAGCAACATCTCGAAGATCCTCGGTAAGTTGTCTTGAGAGCAGAAAATAGATTCGATCGTCGTTTCATCTGACAAATTACTTAAATTACTTTTAGGATCTCGTTGGAAAGGTATGAGCAATTCCGAGAAACAGCCATACTATGAAGAGCAATCACGGCTGAGCAAATTGCACATGGAGCAGCACCCGGACTATCGGTACAGACCACGTCCGAAGAGAACATGTGTGATTGATGGAAAGAAGGTTCGAGTCAACGAATATAAGACAATCATGAAGACTAAAAAAGATTTGATGTGGGGAGATGAGCCAGGATTTTCTCAACCATCAGTAAGTGAACTATTCAAACAATTTTTCAAACCGTAATTTTATCAGGATCTACAGATGGACTTAGCATCACATGTAAATCTTCTCAACGACCTCACACAGCACCATCACCAATCACATCTCCTACAAACAGCTGAAGGTACC
3'HR: CTGCAGCTTTAACCAAGCGTGTCATTCCATCTGATCCTCCAAACATTCTCTGTATTTTTTAAAACCCCCACCTTCGAATTTTGTCGTGTTGTTCTCATGCCAGCTTTACTTTGGTACTCCTCACCCACCACATTTACTTACCTGGTCCATCATGTATTATTACAGTATCTCAATTTTTATTTTTTTTAGCAGATCGGTTTCTTGATTTTCAAAATCTTGGACAATCAAAATCTGCTTGCCGGTGGACCAATGCATTTTTTTTCCATTCATTTTCTCTTTCCTTTCCTCATACAATAGCATAATCTGAAGCGTTATTTCCTTTTCTATGTGTTTCCTTATTGTACCTCTTTCAAAAGCTTCCCACACTCCAAGAATCTCAGCGTTTCCGCATTGTTTCCTACATATTCTGAAGACTGCATTTCAGTTTGATTTACTGTGTTCTTCATATCAATTCCTTTCCTATTCATAATAGGCCTTTTTTCCATTTTTTTTCCTCCTTTTACTGCATTGTGTTCCCATTTTCCACCTGGCACCAGATCTTTTCCTTTATCTTGTTGCAACCTTAGGCTAGTCATCAATCAAACATCCACTCCCGTTTCCATCAAATTTTTACTCTATGCATGCATCTGATTATGGTTCACACTCAATTTTCTGATAAATGTCATATTCAAGAACAAGAGAACAAAACATCATAATATTCGGATTATGGTAGGAAATGTGCATTATGAGTTACAGCCGGAAATGAGAAGATCATCGTAGAATGATTTTGAGAATAGGGGTACAATTGCTATGCAGTTGGAAAAATCAAATGAACCCTTCACACGAAAGTAAGATACATCTTAAATATTTCTTAAAACTTTTCCTTCTAGTTTTCTAATATCTTCTAATAGTACTGCGCTCCATTACATTATTACTAGT
KI verification primers: EGL-13-CKIF: 5'-CACTCCTTTTTAATGGTACGTTCCT-3' EGL-13-CKIR: 5'-GTTTTGTTCCATTCAAAAATTGTCA-3' Download
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Strain name: SYS642 KI background: JIM113 Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; egl-13 (devKi199 [egl-13::mNeonGreen]) X
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Expression pattern: Non-specific
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Lineage expression pattern
350 stage
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600 stage
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3D expression pattern  4-cells  8-cells  15-cells  28-cells  ~50-cells  ~100-cells  ~200-cells  ~350-cells  ~500-cells  ~600-cells  600-cells  bean-stage |
Inferred TF relationships
Based on expression similarity (strong, medium, weak): UNC-42, CEH-48, NGN-1, SPTF-1, CEH-32, FKH-2, EGL-44, CEH-43, ZTF-11, CRH-2
Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)
Targeting: CEH-48, CEH-43, CRH-2, ALR-1, ATF-7, ATHP-1, CAMT-1, CEBP-1, CEH-23, CEH-38, CEH-39, CEH-44, CEH-54, DAF-12, DAF-19, DAF-3, EFL-1, EGRH-1, ETS-5, FOS-1, GEI-3, GMEB-1, HLH-11, HLH-30, LIM-6, LIM-7, MDL-1, MEF-2, MEP-1, NHR-10, NHR-102, NHR-186, NHR-49, NHR-6, POP-1, RCOR-1, SDZ-38, SEL-8, SEM-4, SOX-4, SWSN-7, SYD-9, UNC-62, VAB-3, Y55F3AM.14, Y73E7A.1
Targeted by: ZIP-8, ZTF-11, CEH-8, ELT-2, ELT-3, IRX-1, LSY-2, MDL-1, NHR-85, PAG-3 |
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 Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)
Targeting: CEH-48, CRH-2, ALR-1, ATF-7, ATHP-1, CAMT-1, CEBP-1, CEH-23, CEH-39, CEH-5, CEH-54, DAF-19, DAF-3, EFL-1, ETS-5, GMEB-1, HLH-30, K10B3.5, LIM-6, MDL-1, MEF-2, MEP-1, NFYC-1, NHR-10, NHR-186, NHR-49, POP-1, RCOR-1, SEM-4, SVH-5, SWSN-7, SYD-9, UNC-62, VAB-3, Y73E7A.1
Targeted by: ZIP-8, ELT-2
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