Single-Cell Protein Expression Atlas of Transcription Factors

NHR-10

WormBase description: Is predicted to have DNA-binding transcription factor activity and RNA polymerase II regulatory region sequence-specific DNA binding activity.

Reporter verification: Whole-genome sequencing indicated that the GFP tags another TF, NHR-10 rather than JUN-1
Primers used to verify fosmid reporter: 5'-CAGAAGATGCTGGGGATA-3'

Strain name: SYS576

Cross: OP234 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs234 (nhr-10::TY1::EGFP::3xFLAG(92C12) + unc-119(+))

Expression pattern: Transient-expression

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): ELT-7, END-1, GMEB-1, MDL-1, MED-2, NHR-12, PQM-1, RNT-1

Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: CEH-14, CEH-18, CEH-34, COG-1, HLH-30, LSY-2, NFYA-1, PHA-4, PROS-1, REF-2, TBX-2, UNC-120, UNC-55, UNC-62, MDL-1, RNT-1, ALY-2, BLMP-1, C16A3.4, CEH-82, DPL-1, DSC-1, EFL-1, EGL-13, ELT-2, ELT-3, FKH-8, FOS-1, IRX-1, LIN-13, LIN-39, MEP-1, NHR-85, PAX-3, SKN-1, SOX-4, SWSN-7

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data