UNC-120

WormBase ID: WBGene00006844

WormBase description: Is an ortholog of human SRF (serum response factor). Is predicted to have DNA binding activity and protein dimerization activity. Localizes to nucleus. Is expressed in non-striated muscle and pharyngeal cell.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-TTCCAGATGCCAAACTTCACTC-3'

Strain name: SYS167

Cross: OP659 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs659 (unc-120::TY1::EGFP::3xFLAG + unc-119(+))

Expression pattern: Lineage-specific, Muscle-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): SYD-9, EGL-5, SNAI-1, HLH-1, HLH-8, VAB-7, SEM-4, LIN-39, BED-3, LSL-1, LET-381, M03D4.4, HND-1, CEH-20, PAL-1, TBX-9, HMBX-1, UNC-62, TLP-1, CEH-40, VAB-15, F23B12.7, TBX-8, CES-2, CEBP-1, Y75B8A.6, PHP-3, HBL-1


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: SYD-9, SNAI-1, HLH-1, SEM-4, LIN-39, M03D4.4, CEH-20, UNC-62, TLP-1, CEBP-1, Y75B8A.6, HBL-1, ALY-1, BCL-11, CAMT-1, CEH-13, CEH-14, CEH-18, CEH-2, CEH-38, CEH-39, CRH-2, DAF-12, DAF-19, DAF-3, DMD-5, EGL-18, EGRH-1, ETS-5, FOS-1, GMEB-1, HLH-11, HLH-30, K09A11.1, LAG-1, LIM-7, LIN-13, LIN-22, MBF-1, MDL-1, MEF-2, MEP-1, MML-1, NHR-10, NHR-102, NHR-35, NHR-49, PHA-4, RNT-1, SDZ-38, SKN-1, SPR-4, SWSN-7, TAG-68, TBX-2, TTX-1, UNC-130, UNC-37, ZAG-1, ZTF-11, ZTF-6

Targeted by: HLH-1, UNC-62, HMG-4, LSY-2, NFYA-1, DSC-1, EFL-1, RNT-1, UNC-130

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: SYD-9, SNAI-1, HLH-1, SEM-4, LIN-39, M03D4.4, CEH-20, UNC-62, TLP-1, CEBP-1, ALY-1, CAMT-1, CEH-13, CEH-18, CEH-2, CEH-39, CEH-5, CRH-2, DAF-19, DMD-5, ETS-5, GMEB-1, HLH-30, LAG-1, LIN-13, MDL-1, MEF-2, MEP-1, MML-1, NHR-10, NHR-35, NHR-49, SKN-1, SWSN-7, TBX-2, TTX-1, UNC-130, UNC-37, ZAG-1

Targeted by: HLH-1, UNC-62, HMG-4, DSC-1, EFL-1, LSY-2, RNT-1, UNC-130