Single-Cell Protein Expression Atlas of Transcription Factors

MML-1

WormBase description: Is an ortholog of human MLXIP (MLX interacting protein) and MLXIPL (MLX interacting protein like). Is predicted to have DNA-binding transcription factor activity. Is involved in determination of adult lifespan and negative regulation of transcription by RNA polymerase II. Localizes to mitochondrion and nucleus. Is expressed in excretory cell; hypodermis; intestine; and muscle cell. Human ortholog(s) of this gene are implicated in Williams-Beuren syndrome.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-ACAAACAAACTCTTTCGCATCG-3'

Strain name: SYS450

Cross: OP527 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs527 (mml-1::TY1::EGFP::3xFLAG + unc-119(+))

Expression pattern: Intestine-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): ODD-2, PQM-1, ELT-2, NHR-232, ELT-7, END-1, NHR-102

Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: ELT-2, CEH-18, CEH-31, ELT-3, HLH-1, HLH-30, HMG-4, LIN-13, MLS-2, NFYA-1, PAX-1, PHA-4, SPTF-1, TBX-2, UNC-120, UNC-42, UNC-55, UNC-62, ZIP-8, PQM-1, ALY-2, BLMP-1, C16A3.4, CEH-8, CEH-82, DPL-1, DSC-1, EFL-1, FKH-8, IRX-1, LSY-2, MDL-1, MEP-1, NHR-85, PAX-3, RNT-1, SKN-1, SWSN-7, UNC-130, VAB-15

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data