ELT-7

WormBase ID: WBGene00015981

WormBase description: Is predicted to have DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II proximal promoter sequence-specific DNA binding activity. Is involved in endodermal cell fate determination. Localizes to nucleus. Is expressed in E lineage cell.

KI information: mNeonGreen knockin at N-terminus

sgRNA1: TTCATGATAGTCGTAACAGA

sgRNA2: GTTGAAGAGTAGTAGTTTCA

5'HR: GCATGCTAATGAATTTAAAATCGCCTCTCACAGTTCTGAATTCTTATCTTTTTCCAAATCTACACTTTTTTTAATAGAAAAATACAGTTTACGTAAAACTACATTCCATGTTGATTTTTCAAGCATTCATTTTAAATAAATTATATGTATTTTAAAAAAAAACACGTTAGAAATAGGCTACCGATAATACAAGAATTAAATTATAAACACTATGCACTCTCACAATCAGTTTATGCCCCTTTTGCACATTACTTCAACTTAAAAAAAAATAATTGCACACTTCCTCCAGGACACAGCATTTCAACTTGGAACAAGATTTCCCATTCGCGACAAATTTGTCCAATTTGCCATTTGTTAAGCTCGGAATACTCTAAAATTTGCCTTGAGTTTAGAAGAAAGAAATGAATGAAATAACACAAAATTTTGTGAGTATCTTTAAAAAAAATTTTTTTTCCAGAATGCTACACTTTTAAAGCTTTTTCGTCAATTTTGTGATCCAAAAAATAGTTTTAAAATATCGAGATTTTCCGGGTTCGCCGACCAGGGCAAATTTGTCAAATTTGCCGGTTTTGCCGGACTCTGCAAATTTTGAGATTTGCCGCACCCCCCCCCCCTCTTTCCCATGTTTCCTTTTTTTGCTCGTCCACTGGCACACAAGGCATAGGTACATGCATCTCTGACTTACCACTAATTTACGACTATCCATAAAAGTCCCAAGTCATATTTACTTTCGTTCTCCTTCCCTTTAAGAACTTTGCCGTCCAGGTTCATAGTCATTATATCATAAACTACTAATGATGGGTGGTGAGCCACACACAGTTCCCCATCACCACCGCCGCCGGTCTATCTTATCACTCTGACCTTTTTTGTCTCTTTCTCTAGGATCTCTCCGGGCTCTTTGTTACTTTCCTGCCTGTAACAAAGACAGACATTTGTGGTTAGAGTTGTCTGCTCTAGTCGACTGGAAAAAGGTACC

3'HR: CTGCAGATGCTTCCAGAGACCACCACTCTTCAACCACTTCCATCCGTCACCACCATTATGAATGAGCCGCCAGCGAGAAGTCCAATGGAGGACTATTATTTATTTAATCAATTTCAATATAATCAAAAGTAAGTTTTGAAATCAAAACTGTTTTATTGCAGTGCATAAAAATGTTGCATAATGTAATTGTATTATTTTTACGGCAAACTTAAATTGAAATATTCACTATCAACAGAATTATATGGTTTTTACATTACTACCAAAATATTACTTTCCTGTAGTAATGTATACAAAAACAACAACTTTGTTCCAACATGTGACCAAAACTATGTTTTATTGAACTCATGTAGCATAACTGATAAAAATCAATATCGAATGCTCCAAAGTATACTGCTTATAATCAGTAGTTTTCAGTGTTTTTGAGAATAGGAATCTTTGAGAATTATTATATTTTTTAAGGTTTATTATTATAATTTGAGAGTCTGAAAAACGAGTTTCATGCTGGGAATGGCTTAAAGTGTATGACTTAACATGTTTCCTGTTTGGCAATTGTAGGTAATTTCCAACTTTCTCTCAAAGTTTAACAATTTCCTACAAAGCAGTTAAAAAATTCTGTCTACCTTTCGTAACTTTCTTTCAAATGTTAACACTTTTTGCAAAATTTTTGCAACTTTCTGTCAATCTTTGGACATTTTCTGACAACTTTTTGCAACTTTCTGCCAACTTTTGATAGCTTTCTTTTAAAGTTTAACAACTTTTTGCCAACTTTTGGCATCGTCCTGCCAAGCTCTGGCAACTTTTTGACAACATTTGGCAACTTTTTTCCAACTTTTGGCAACTTCTTGCAAAACTTTTGGCAACTTTCTGACAAATTTTAACAACTTTCCGCTTAACTTTTCCTGCCAAATTTTACCAACTGCTTACAAATCTTTTGCCTAGTCTCCTGCACCACAAATGTACTACAAACTCAGCACTAGT

KI verification primers: ELT-7-NKIF: 5'-ACGCACATTGGGGGTTAGTC-3'ELT-7-NKIR: 5'-AGCAGTGTGAGCAGCACGC-3'

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Strain name: SYS543

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; elt-7 (devKi159 [mNeonGreen::elt-7]) V

Expression pattern: Lineage-specific, Intestine-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): PQM-1, MML-1, ELT-2, MDL-1, END-1, NHR-232, END-3, NHR-102, DVE-1, NHR-12, ODD-2, DPFF-1, PHA-4, TBX-9, ATF-7, CEH-20, CEH-74, SEL-8, F23B12.7, LAG-1, NHR-10


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: ELT-2

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: ELT-2