NHR-12

WormBase ID: WBGene00003611

WormBase description: Is predicted to have DNA-binding transcription factor activity.

Reporter verification: Whole-genome sequencing indicated that the GFP tags another TF, NHR-12 rather than CEH-30.
Primers used to verify fosmid reporter: 5'-ATTATGCGTTATGTTGTTCTGG-3'

Strain name: SYS93

Cross: OP120 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs120 (nhr-12::TY1::EGFP::3xFLAG + unc-119(+))

Expression pattern: Lineage-specific, Tissue-specific (Skin and Intestine), Transient-expression

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): C16A3.4, MDL-1, ELT-3, ELT-2, PQM-1, ELT-7, END-1, RNT-1, NHR-102, NHR-232, GMEB-1, NHR-23, CEH-16, NOB-1, LIN-26, CEH-40, REF-1, DVE-1, BAZ-2, HAM-2, ELT-1, NHR-25, CEH-20, HBL-1, VAB-7, TBX-9, ATF-7, TLP-1, TBX-11, UNC-62, TBX-8, F23B12.7, CEH-74, NFI-1, SEL-8, CEBP-1, LAG-1, Y75B8A.6, NHR-10, CRH-2


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: NHR-102, CEH-38, ETS-5, LIM-7, ZTF-6

Targeted by: MEP-1, PHA-4, UNC-55, BLMP-1, EFL-1

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: ETS-5, ZTF-6

Targeted by: MEP-1, PHA-4, UNC-55, BLMP-1, EFL-1