VAB-7

WormBase ID: WBGene00006873

WormBase description: Is an ortholog of human EVX1 (even-skipped homeobox 1) and EVX2 (even-skipped homeobox 2). Is predicted to have DNA binding activity. Localizes to nucleus. Is expressed in muscle cell; neurons; somatic nervous system; and tail hypodermis. Human ortholog(s) of this gene are implicated in several diseases, including WAGR syndrome; diabetes mellitus (multiple); and eye disease (multiple).

KI information: mNeonGreen knockin at N-terminus

sgRNA1: CACCTTATTAACCCCAATAA

sgRNA2: TTCTACAAAAGTAAAAAACA

5'HR: GCATGCATCAAATTTTTCCGAGTTTGTTAAGCACGGCGAATTTGCCGAATTTGCCGAATTTGCCGTGCTCGGCAAATATTGAAAAAAGAGATTTGCCGAATTTGCCGAGCTCGGCAAAAGTGGCCTAGAAAATTCAAGTTAGGCCACCTGTCAATTTGAATGTTGAATTTTTATGTTGAAAATACTTACATAAGTTTTTTCATCCGTTTCACCTAGCTAAGCACATGGCCTAGAAAAATCAAAATTAGGCCACCGTAAAAGAGAAAATAATTTGTATATTAATATGAAACATTGAATGAGCTAAGGGGCTCCAGTTGCTTTGTGGCCTAAAAAATTTGAACCCTCGGCCACCTCCGTGAAATTTTCGAAAATTTTCATTTTGAATTTCATCGTAACGGCCGGGAACTTTAGCTTGATCACGTGGCCTAGAATACTGAAGTTTTTTGAGAACCGTTATTGGGGAACTTTCACGCAGGTACCTCGTGGGTATTAGGAAACATGGTCTAGAAAAAAAATTAGACTGTCTGCAAAATAATTTTAATCATTGTGTAATATTCTTCATTGTAAGGGCCCTAGAACCTTTACTTGGTTACGTGGCCTGGAAAACTCAAAAATTCGGCCACTAATTGGTTTTTTGAAGTTTTACTTGAAAAAAAAAATGACCTGATGTTTTTGAAAAGCAATACATTTTCTCTTAATATTAAATTTCCAAAAAAATGCCAAATCTGCCGTATTTTAAATTTCAAACGTCATGGAAAAAGATTATTATCTAAAGTCTCTTTCTATTCCAGCACTCCTTCCCCTCTTCTATTGGCCGCCCTTCTCATCTGAGCGTGGGGCGTGGCCAAATCCACCGTAGTCACCGAGAGGTTGAGCCCCGCCCAGCGCCTTATTCTATTGTTTCCTTTATCTTTGAGTTTTAGTACTCAAGTTCTTTTATTTGAAAAAAAAACTATCAAAAATTTTAAAAATTAGCACC GGT

3'HR: CTGCAGATGCAATCATTTGATATTGAGTCTCTTATCGGGGTTAATAAGGTGAGATTTTTTTGTTTTGTATTTTGTTTTGAGAACGATGTTTTATACTTTTGTAGAACTGTTATTTAATGTCTGTAAACTTCTTGTTGTCAAAGATCCATTAAAATTAAAGTTTCTAAATTTTTGTAGTTTTTTAGGGATTTTTTCAACTTTTTTGATAACTGTAATAGGTAGAAATAAAAAATTATATAATTTCATGCAATAAAATTTAATGAAAAATTATTAATCTAATCATAAAAGTTTAGAAGATTTATGTTGCTAAAATTTTATTTGATAACTGTAAAAGTTTCATTCAGTTTCACAATTTTCAAGATTATCGGGCGTTTGAAGCTTAAAAGATAATTTTTTTAAAGTGTTTAAAAGTGGTTATAACTTGGTTTGTAATAATGCAAGCAAAAAATTTTCAACAAACAATTTGTTCATTATGTTTTAAGCTATAATTTGTTAGTTGTCAACTTTTTGATAACTGCATTAGTTGCAGAGTTATGGACAATTTAATTAGGTTGCATAATCAAAAAGTGTCATATTGCATAATCTCAGCTATAATGCATAGTTGCATAGTTATTTGAATTATTGAAACATTTAAATTCTAATTTTTTAAAATCTGTTTTGCTAGAAGTTTTTAAATTATTCTTTAAACTATTTTCTTTTTTATTAATACTTTGAAATTTTGATTTAAGAAATACAGAAAAAAAAATTAAAATCGTTTTTTGAAAAGTTGAAATTACCGAAAATTATCTATTTTGACAGCCAAACTTCAGTTATTTCTAGTATTCAAATTAATTCCAATTGTAGTTGAAAAATAATTATGTCTAGTAAAACAATGTCGATTTTATTTTCAGATCAAAAACTGTTTACATAAAAAAAGTTGTTTTTACCTCTTTTAATCTAGAAAAATTTGAAAAAATATATCATTGAAACTTTTTCGACACCCTAAAGTCTTTTAGCACTAGT

KI verification primers: VAB-7-NKIF: 5'-CTACGCGGAACTTATTCAGAAACC-3' VAB-7-NKIR: 5'-CTGTCCGGGGGTCTTCAGAG-3'

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Strain name: SYS510

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; vab-7 (devKi142 [mNeonGreen::vab-7]) III

Expression pattern: Lineage-specific, Muscle-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): EGL-5, SYD-9, PAL-1, HLH-1, PHP-3, UNC-120, LIN-39, TLP-1, HND-1, NOB-1, M03D4.4, VAB-15, CEH-20, HMBX-1, NHR-12, TBX-9, CEH-40, COG-1, BAZ-2, NHR-67, UNC-62, HBL-1, Y75B8A.6, F23B12.7, HAM-2, CEBP-1, CES-2, ATF-7, TBX-11


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: UNC-62, CEH-36, CEH-43, LSY-2, MLS-2

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: LSY-2