BAZ-2

WormBase ID: WBGene00001470

WormBase description: Is an ortholog of human BAZ2B (bromodomain adjacent to zinc finger domain 2B). Is predicted to have DNA binding activity and metal ion binding activity. Is expressed in several structures, including AB lineage cell; P9/10L; hermaphrodite distal tip cell; neurons; and vulva.

KI information: mNeonGreen knockin at N-terminus

sgRNA1: GAGATGATGCAGTGGCCTTC

sgRNA2: GATGAGTTATCACTCATTTT BAZ-2-NKIsg3: TTTCGATGGAGATGATGCAG

5'HR: AAGCTTTTATTGAACTAAGTCTAGAAACTTAATTTTTTTAATCTCGTGTTTTTTAAAAATAATTTTATTCGATCTACAATTTTACCAAAATCACCGCTTCCCTCATATGGCATCTGAACCCTCCCTTCCTCCTACATAAATTTCTCAATACGTACCAAAATATTTCATCACCAAGTAACCGCATACACCATTCGCATTTGTCCTCCTATTCCTCCACTTGTCCTCTTCTTTACTTTTCATCCACTTTTTGCGTCTTCCAAATATCGTTCTTTACCAACTGTGAGTGAGAGAGAAGGGTACCTCCTGCCGCCTGCTGCTGTAGCCATTTCCCTCTCTCGCTGTGGTTCAGTATACTCGCTGATTGACACATCTGTCCTTTTTCATTCCCTCCGTGTTGTCCTGGTCATCGTTCAATCGTTTTCCTTCATATTCTTCCTCACCATATCTTTCTCTGCTTTGCCTTCGTTCAATTTCTGATGGTACGATTTTGTTAAAGTGTTTCACGGGACAAACTTTTGAAGTCGCGACGTTTTCTAGAACCGCATGAACTCAAATTGTCACATGCATCTAAATAGACAACAATTTTTGCTCACCTTTCTTTAAAATTTCGATATGAATTTCCATAATTTTCAAAGTTCTATTCTACTAAACTTAATAAAGACTAAGCATGCAAACCTGAACCTAAAAACTTGAACGTAACCTAAGCTATGAGCGAAAACTGTTGTCAATTTACGAATGCATATTCTCGCGACATAATGATATTACTCCTTAATAATACATAGAACACTCATGCTCACAGACCTCTAGATTCAAAGGCCTTTATGGTGTCTGACTATTTTTGTCTTCATCACACATTTTTCGAAATTCTCACTTTTTATTCAACTTCTTGACCCTTTTAACTTTCATTTAATATTGCTAAATTTCAGAATTGTACATGTAAATTCTTTCCAAAAACC GGT

3'HR: CTGCAGATGAGTGATAACTCATCTAATCAGTTTCTACTTCTGCTAGCAGCTGCACAGCAACAACAGCAACAACAACTTCTACAGCAACAACTTGCAAAGATTCAGAAGGCTACTGCCTCATCTCCATCGAAATCAACTAATGGAACATCAGCATCAACGTCGGCTGTCCCGTCAACTTCTGGGACATCGTCATCTCAAAATGAAGCAGCTCAGTTGCAAAATTTAGCAAAAATGCAGCAAATTCAGCAGCTGGCCCAGTTTGGAGCACTCATGGCTGCGCAGAAGAAACAACAAGAAAAAGCGGCAGCTGATAAGGCAAAGGAAAAAGAAAAGGAGAAGCAAAAGGCTGCCGCAGCAGCTGCCGCCGCCGCAGCAAAAGCCTCAGCGTCTACTTCCTCCGCATCAGCAATTCCTGGATTGTCTCCAGAAATGCTCGCAGCATGGCAACAAGCAATCCAGATGCAAGCTCTCCAACAAATGATGATGACTCCACAGAAATCTCAAATGGAAGAAGCTATCAAGAAAATGATGGATATGGCAAAGAAGAAGCCAGCTGGAGTCGCCTCAACAAGTTCAGCATCTACAAGCTCATCCACACCATCCACATCATCAGCTTCAATTACATCATCTAATAACAATGCAGCGAACAACGCTGCGAGCAATATGATGAACAATGTGATGTGGCAATTGGTGGCAGCACAAATGCAACAAAAACAGCAACAACAACAGAAGGATACTCAGAAAAAGGCGGTAAGATATTGCTTTTTATATTATTTTTGAGAAAAATGTTGAATACTTTCAGGATCAAGCGAAAAAAGCGAAAGAGCTTGCCAAACAACAACAGAAAGAGCAAGATGTGAAGAATAAGCAACAAGAGGAGATTTTGAAGTTTCTTATGGCTCAACATCAGCTGAATCATCAGAAAAAGCATGAGAAGAAACTAGT

KI verification primers: BAZ-2-NKIF: 5'-GAGGTAGTGGTGACCATGAAAGAGTA-3' BAZ-2-NKIR: 5'-TTCTTCAGGACTGTCCGATTCG-3'

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Strain name: SYS677

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; baz-2 (devKi214 [mNeonGreen::baz-2]) III

Expression pattern: Non-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): RNT-1, GMEB-1, CEH-10, NHR-12, EGL-5, CEH-40, LIN-26, NHR-23, PAG-3, NOB-1, Y56A3A.28, HAM-2, CEH-16, UNC-130, Y48G1C.6, NHR-25, HBL-1, CEH-20, VAB-7, ATF-7, LIN-1, VAB-3, UNC-62, ELT-1, Y75B8A.6, CEBP-1, F23B12.7, TBX-8, NFI-1, CES-2, CRH-2, LAG-1, IRX-1, SEL-8


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: NA

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: LIN-32