NHR-25

WormBase ID: WBGene00003623

WormBase description: Is an ortholog of human NR5A1 (nuclear receptor subfamily 5 group A member 1) and NR5A2 (nuclear receptor subfamily 5 group A member 2). Exhibits armadillo repeat domain binding activity. Is involved in molting cycle; positive regulation of nematode male tail tip morphogenesis; and positive regulation of transcription by RNA polymerase II. Localizes to nucleus. Is expressed in several structures, including excretory system; gonad; hypodermal cell; tail; and vulval precursor cell. Human ortholog(s) of this gene are implicated in 46 XY gonadal dysgenesis; premature ovarian failure; and spermatogenic failure 8.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-CCACCCTGATGAGATGGGAATA-3'

Strain name: RW10348

Strain genotype: unc-119 (ed3) III; stIs10116 (his-72p::his-24::mCherry::let-858 3'UTR + unc-119(+)); itIs37 (pie-1p::mCherry::H2B::pie-1 3'UTR + unc-119(+)); stIs10318 (nhr-25::TGF(3H4)::GFP::TY1::3xFLAG)

Expression pattern: Lineage-specific, Skin-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): LIN-22, ELT-3, RNT-1, NOB-1, NHR-23, LIN-26, BLMP-1, NHR-35, HAM-2, GMEB-1, EGL-38, NHR-12, CEH-16, ELT-1, CEH-5, PROS-1, CEH-40, UNC-3, VAB-15, PHP-3, BAZ-2, LIN-1, REF-1, Y56A3A.28, UNC-130, LIN-39, HBL-1, NHR-102, CEH-41, Y75B8A.6, NFI-1, CEH-13, UNC-62, TBX-9, HLH-30, CEH-43, CEH-20, IRX-1, VAB-3, PAL-1, TBX-8


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: RNT-1, BLMP-1, HAM-2, ELT-1, UNC-62, CEH-31, CEH-36, ELT-2, ETS-7, HMG-4, LSY-2, TBX-2, UNC-55, ZIP-8, ELT-3, IRX-1, C16A3.4, MDL-1, MEP-1, NHR-85, PAG-3, SWSN-7

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: BLMP-1, ELT-1, CEH-31, CEH-36, ELT-2, ETS-7, HMG-4, LSY-2, ZIP-8, ELT-3, IRX-1, C16A3.4, MDL-1, MEP-1, NHR-85, PAG-3, SWSN-7, TBX-2