HAM-2

WormBase ID: WBGene00001821

WormBase description: Is predicted to have DNA-binding transcription factor activity. Is involved in cell fate determination; cell migration; and regulation of neuron differentiation. Localizes to nucleus.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-TGTTTCAGGTAGCCAGAGGG-3'

Strain name: SYS553

Cross: OP639 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs639 (ham-2::TY1::EGFP::3xFLAG + unc-119(+))

Expression pattern: Lineage-specific, Skin-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): RNT-1, ELT-3, C16A3.4, LIN-22, NHR-23, NHR-35, BLMP-1, LIN-26, PAG-3, GMEB-1, NHR-12, NHR-25, CEH-16, CEH-40, CEH-5, BAZ-2, NOB-1, MBF-1, Y48G1C.6, AST-1, REF-1, LIN-39, ELT-1, Y56A3A.28, HBL-1, NHR-102, PHP-3, LIN-1, UNC-130, COG-1, Y75B8A.6, PAL-1, VAB-7, CEH-20, VAB-3, SOX-2, CEH-41, NFI-1, HLH-30, TBX-8, CRH-2, CEBP-1, UNC-62, LIM-7, F23B12.7, MLS-2, CEH-43


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: RNT-1, NHR-23, NHR-35, BLMP-1, NHR-25, AST-1, ELT-1, HBL-1, NHR-102, UNC-130, Y75B8A.6, CEH-20, VAB-3, CRH-2, CEBP-1, UNC-62, LIM-7, CEH-43, ALY-1, ARID-1, BCL-11, CEH-48, CES-2, DAF-12, DAF-19, DAF-3, DMD-5, DVE-1, EFL-3, EGL-18, ETS-4, ETS-5, FOS-1, GMEB-2, HLH-2, K09A11.1, LIN-13, MDL-1, NHR-49, NHR-85, POP-1, PROS-1, SEL-8, SKN-1, SNAI-1, SWSN-7, ZFH-2, ZTF-30, ZTF-6

Targeted by: ELT-1, UNC-62, PHA-4, SPTF-1, ZIP-8, BLMP-1, LSY-2, MDL-1, MEP-1, NHR-85

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: NHR-23, NHR-35, BLMP-1, Y48G1C.6, ELT-1, Y75B8A.6, CEH-20, VAB-3, CRH-2, CEBP-1, UNC-62, CEH-43, ALY-1, ARID-1, CEH-48, CES-2, DAF-19, DMD-5, DVE-1, EGL-18, ETS-5, GMEB-2, HLH-2, LIN-13, MDL-1, NHR-49, NHR-85, POP-1, SKN-1, SNAI-1, SVH-5, SWSN-7, ZFH-2, ZTF-30

Targeted by: ELT-1, UNC-62, PHA-4, SPTF-1, BLMP-1, LSY-2, MDL-1, MEP-1, NHR-85