EFL-3

WormBase ID: WBGene00009899

WormBase description: Is an ortholog of human E2F7 (E2F transcription factor 7). Is predicted to have DNA-binding transcription factor activity, RNA polymerase II-specific; sequence-specific DNA binding activity; and transcription factor binding activity. Is expressed in VA neuron; VB neuron; head neurons; and ventral nerve cord.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-TGGCGATGGAAATAGTTGGG-3'

Strain name: SYS449

Cross: OP344 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs344 (efl-3::TY1::EGFP::3xFLAG + unc-119(+))

Expression pattern: Transient-expression

Lineage expression pattern

350 stage


600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): CEH-10, DMD-4, UNC-3, LIN-32, UNC-86, CEH-31, HLH-30, VAB-3, SEM-2, CEH-30, UNC-30, Y56A3A.28, CND-1, SOX-2


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: EGL-18, ELT-1, MDL-1, TLP-1

Targeted by: LIN-32, SEM-2, CND-1, ALY-1, BLMP-1, CEH-2, CEH-34, CEH-36, ELT-1, ELT-2, HAM-2, HLH-1, HMG-4, LIN-13, MEP-1, MLS-2, NFYA-1, PHA-4, REF-2, SPTF-1, TBX-2, UNC-55, ZIP-8, UNC-86, LIN-39, NHR-85, RNT-1, ZTF-11

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: EGL-18, ELT-1, MDL-1, TLP-1

Targeted by: CND-1, ALY-1, BLMP-1, CEH-2, CEH-34, CEH-36, ELT-1, ELT-2, HLH-1, HMG-4, LIN-13, MEP-1, MLS-2, NFYA-1, PHA-4, REF-2, SPTF-1, TBX-2, ZIP-8, UNC-86