WormBase ID: WBGene00004949
WormBase description: Is an ortholog of human SOX14 (SRY-box transcription factor 14). Exhibits RNA polymerase II regulatory region sequence-specific DNA binding activity. Is involved in neuron fate specification. Is expressed in several structures, including head neurons; hypodermis; inner labial neurons; neuroblasts; and rectal epithelial cell. Human ortholog(s) of this gene are implicated in microphthalmia.
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KI information: mNeonGreen knockin at C-terminus
sgRNA1: TTTCGAGAAGTGTGTTTAAG
sgRNA2: GTACCAAAAAAACTAATTGA
5'HR: GCATGCTTTAGAGTCTCTGATCCCTTAACTCACTTGAGTTAATCCTCTACATTTAAAATTATTTTTCTCATATTGCATTGAAAATCTGGAGAAAATAAGTATTTAGGTTCTATTCACAGAAAGTTTTAGTCATATTACCTGTGTTCGCCTAAATTTCAACATTTCGAACAGCTTGAAAGCAAACTGCATTTCACGTATCTTACATATTATATTACACTTTCATTATTCTAGGAAAATCCAAAAATGCACAATTCTGAAATCAGCAAACGTCTTGGGACAGAATGGAAAATGCTCAGTGAACAAGAAAAAAGACCATTCATCGATGAAGCGAAACGACTACGCGCCATTCATATGAAGGTTAGTTGGGGAATCTGGGTCGCCTGATACTAAGCTTATTGAATAGTGCCATCACAATTTGTTCTCTTCAGGAACACCCAGACTACAAATACAGACCGAGAAGGAAGACAAAGTCAATTAATAAGAAAAATGGAGCACCAATTCCATTTGGAAATTTGGACACAAAAACGCCATCTGTAAGTTTTCCTTCGATATTGAATACTTCATATATCTATTTTCATGTTTCAGTATCCGACGTTAACAACCAACTGGAATGCGACAAATCAGTATATTGATCAGTTTCGGTTTGCTCCGTACCCCACAACAACTGTAATGGATCAAATTCCATTCTCGTTGACATACCCAGTGCATTCGGTCCCAACTGACAACTCGTCGCCTTCGCAGTTCCAGCCATCTCCGATGAGCACAAATTTTGCTGGCAGCTACCTGACACCAAAATCAGAGAGCTCGCCGGTTGGCAGTGATTCTACAGTCGGCACCGTTGACTCCAGTCAGTTTCGTGCTTACTACGACCACACAAAGGATCAGATGATGTATCCGTACAGTATTGAATTGACTCATGCTCAAAACATTCAAAATGCTCTTTCCCAATCCCATGTTACCTCTGGTACC
3'HR: CTGCAGACACACTTCTCGAAAGAAATTGACGTTCAATTAGTTTTTTTGGTACAAATTTCAATACTCCATTAATTTTTTTCAAAACGGTTTTTGACTTTTGAAACATCTGAGCTTTTTCAATTCCAAAATCAAAATCTGAGGCAGTTTCAGAGATGTGCATTGCTAAATGACTTTTTACCTCTTATTCCCATTTCTCTCACAAAAAATTCAATGTCCCGTGTAGGGTGATACGTGCCCAGAGGTTGTTTCACTTCCCCATTTTTTCTCCCTTTAAAATTTTGCCAACACAACACTGACTTGGTATTTGACTGTTTTTGAAATTCTAATCAATATGTTTCGTATATTTGTTTCCTATGTCAGTTTTTGTGGTTTATCGGAATTCATTTTCTCCGTCCTGATTCTTGATAATTCTCACTGTTCTTCGATTTTTGTTTGCTCTCTTACCCATTATTTGATGCCACTTGTGTCAGCCGTCATTTTCCACGTTTCCCCAGTTTTTTAATCCATGCAACCAATTTATTGCTCCTAACTCTCATCACCTTCAGTTTTATTGTATATTTCTTTCTTGAAACTCCAATAATTATTCGCGCTTAGAAACCTATTTAGATGCGTCCCAACTCATTTCTGAGGACCTCATATTTCAGTTTTGCATTCTGAAATACCATGTTCTAAAATTTAGATGTTGATACCATCATATAAGTTTACAAATTTTTAATTTATCCATAAAATGTTGACACCCAAGCTTAGATCTGTAAAACCAAACGACTAAAAAAGATTGAAGCAAGAAGCAGTCAGTTTCCGGAGGCTTCTTTTTTTTCACCCAGATTTGATAAAAAAGATGCATTGGTATTTTTACTTGCACACAACCAACCAAAAGTGTGTTCTTTACTTGATTTGGTCGTTACCGAGAAGTTTATTTTGTTCAAACAAGCAATGGTGAAGTGAATGTATCAGAGGCAGACGAGAACTAGT
KI verification primers: SOX-2-CKIF: 5'-TCGTGACCTTCTTGATTCACTTATG-3' SOX-2-CKIR: 5'-CCACACAGGAAGAAACAGTATGTATG-3' Download
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Strain name: SYS516 KI background: JIM113 Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; sox-2 (devKi153 [sox-2::mNeonGreen]) X
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Expression pattern: Lineage-specific, Neuronal-specific
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Lineage expression pattern
350 stage
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600 stage
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3D expression pattern  4-cells  8-cells  15-cells  28-cells  ~50-cells  ~100-cells  ~200-cells  ~350-cells  ~500-cells  ~600-cells  600-cells  bean-stage |
600 stage
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3D expression pattern  4-cells  8-cells  15-cells  28-cells  ~50-cells  ~100-cells  ~200-cells  ~350-cells  ~500-cells  ~600-cells  600-cells  bean-stage |
Inferred TF relationships
Based on expression similarity (strong, medium, weak): CEH-5, CFI-1, FKH-10, PAG-3, CEH-8, TTX-3, PROS-1, TAB-1, MBF-1, CEH-32, AST-1, UNC-39, CEH-31, UNC-3, SEM-2, HLH-30, Y56A3A.28, ZAG-1, CEH-30, CEH-48, CND-1, NHR-67, VAB-3, REF-2, Y48G1C.6, MLS-2, CEH-6, HAM-2, CEH-41, LIM-7, EGL-44, ELT-6, SPTF-1, ATF-7, CES-2, CEH-45, LIN-32, NGN-1, ZTF-11, HLH-2, EFL-3
Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: CEH-31, CND-1, REF-2, CEH-34, HMG-4, LSY-2, NFYA-1, PHA-4, FKH-10, DSC-1, SOX-4, SWSN-7, UNC-86 |
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 Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: CEH-31, REF-2, CEH-34, HMG-4, LSY-2, NFYA-1, PHA-4, DSC-1, SOX-4, SWSN-7, UNC-86
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