PROS-1

WormBase ID: WBGene00000448

WormBase description: Is an ortholog of human PROX1 (prospero homeobox 1) and PROX2 (prospero homeobox 2). Is predicted to have DNA-binding transcription factor activity, RNA polymerase II-specific. Is expressed in excretory cell; labial sensillum; neuronal sheath cell; and neurons.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-CAGGAGATGAAGTTAGGCAATGG-3'

Strain name: SYS176

Cross: OP500 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs500 (pros-1::TY1::EGFP::3xFLAG + unc-119(+))

Expression pattern: Neuronal-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): CEH-8, CEH-5, MBF-1, TAB-1, UNC-39, NHR-25, SOX-2, CEH-32, HLH-30, CES-2, Y75B8A.6, ELT-6, HLH-2, CRH-2


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: HLH-30, CRH-2, BED-3, BLMP-1, CAMT-1, CEBP-1, CEH-38, CEH-43, DAF-12, DAF-19, DAF-3, EFL-1, EGRH-1, ETS-5, HBL-1, LAG-1, LIN-13, MDL-1, NHR-10, NHR-102, NHR-23, NHR-49, POP-1, SDZ-38, SWSN-7, UNC-37, ZTF-6

Targeted by: CEH-6, CND-1, HAM-2, LSY-2, M03D4.4, MLS-2, NFYA-1, SPTF-1, BLMP-1, EFL-1, RNT-1

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: HLH-30, CRH-2, BLMP-1, CAMT-1, CEBP-1, CEH-43, CEH-74, DAF-19, EFL-1, ETS-5, LAG-1, LIN-13, MDL-1, NHR-10, NHR-23, NHR-49, POP-1, SWSN-7, UNC-37

Targeted by: CEH-6, CND-1, LSY-2, M03D4.4, MLS-2, NFYA-1, SPTF-1, BLMP-1, EFL-1, RNT-1