Single-Cell Protein Expression Atlas of Transcription Factors

EFL-1

WormBase description: Is an ortholog of human E2F5 (E2F transcription factor 5). Exhibits protein C-terminus binding activity; protein N-terminus binding activity; and transcription factor binding activity. Is involved in several processes, including embryonic digestive tract morphogenesis; negative regulation of Ras protein signal transduction; and negative regulation of vulval development. Is expressed in gonad.

Reporter verification: Verified by comparing expression pattern to the literature (Page et al., 2001)
Primers used to verify fosmid reporter: nan

Strain name: SYS230

Cross: YL424 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; vrIs68 (efl-1p::efl-1::GFP::FLAG::efl-1 3UTR + unc-119(+))

Expression pattern: Ubiquitous

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): NA

Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: ALY-1, ARID-1, ATF-7, ATFS-1, ATHP-1, BCL-11, BLMP-1, CAMT-1, CEBP-1, CEH-20, CEH-38, CEH-39, CEH-40, CEH-43, CEH-44, CEH-48, CEH-83, CEH-88, CEH-9, CRH-2, DAF-12, DAF-19, DAF-3, DMD-5, DNJ-17, DPFF-1, DUXL-1, EGL-44, EGRH-1, EOR-1, ETS-5, FOS-1, GMEB-1, HLH-1, HLH-11, HLH-2, HLH-30, HMG-20, K09A11.1, LAG-1, LIM-7, LIN-1, LIN-11, LIN-13, LIN-15B, LIN-39, LSY-2, MBF-1, MDL-1, MEF-2, MEP-1, MML-1, NFI-1, NFYA-1, NGN-1, NHR-10, NHR-102, NHR-12, NHR-23, NHR-35, NHR-49, NHR-6, NHR-85, PHA-4, POP-1, PROS-1, RCOR-1, SDZ-38, SEL-8, SEM-4, SKN-1, SMA-2, SPR-4, SWSN-7, T07F8.4, TAG-68, TLP-1, UNC-120, UNC-37, UNC-42, UNC-62, Y55F3AM.14, Y73E7A.1, ZIP-3, ZTF-6

Targeted by: CEH-14, CEH-18, CEH-19, CEH-31, CEH-34, CEH-39, CEH-48, DVE-1, ELT-2, ELT-3, ETS-4, HLH-1, HLH-30, K09A11.1, LIN-13, LSY-2, MEP-1, NFYA-1, PAX-1, PHA-4, PQM-1, PROS-1, REF-2, RNT-1, SPTF-1, TBX-2, UNC-42, UNC-55, UNC-62, ZTF-11, AHA-1, ALY-2, BLMP-1, C16A3.4, CEH-8, CEH-82, CEH-90, DPL-1, DSC-1, EGL-13, FKH-10, FKH-4, FKH-8, FOS-1, IRX-1, LIN-39, MDL-1, MEF-2, NHR-85, PAX-3, SKN-1, SOX-4, SWSN-7, UNC-130, UNC-86, VAB-15, ZAG-1

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: ALY-1, ARID-1, ATF-7, ATFS-1, ATHP-1, CAMT-1, CEBP-1, CEH-20, CEH-39, CEH-40, CEH-43, CEH-48, CEH-5, CEH-74, CEH-83, CEH-88, CRH-2, DAF-19, DAF-3, DMD-5, DNJ-17, DPFF-1, DPL-1, DUXL-1, EGL-44, EOR-1, ETS-5, GMEB-1, HLH-1, HLH-11, HLH-2, HLH-30, HMG-20, K10B3.5, LAG-1, LIN-13, LIN-15B, LSY-2, MDL-1, MEF-2, MEP-1, MML-1, NFI-1, NFYA-1, NFYC-1, NHR-10, NHR-12, NHR-23, NHR-35, NHR-49, NHR-85, POP-1, PROS-1, RCOR-1, SEM-4, SKN-1, SMA-2, SPR-4, SWSN-7, T07F8.4, TLP-1, UNC-120, UNC-37, UNC-42, UNC-62, Y56A3A.28, Y73E7A.1, ZIP-3