WormBase ID: WBGene00012209
WormBase description: Is an ortholog of human HMG20A (high mobility group 20A). Is predicted to have DNA binding activity. Is expressed in body wall musculature; head neurons; seam cell; ventral nerve cord; and vulval muscle. Human ortholog(s) of this gene are implicated in mitochondrial DNA depletion syndrome 15.
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KI information: mNeonGreen knockin at N-terminus
sgRNA1: AGCTTCATCCTCTTCGACGT
sgRNA2: TTTCGCGGCGGGACCCGTTG
5'HR: GCATGCCAGAATATTAAAAAATTCAAGCCGCGATGCGACCGCGCCGCACTGGGATCAAAAGGCGCGAGTTTAAATTTCAAAGAGCGATTTTTAAAGTTTTTCAAAAAATACTAAATACATAATTTTCTTTTTCAAAATTTTATTAATCTCATCGCATTTTTCTTCCGTACAGCTGCCGTCTTTTTCTCTGCGCTCTCTCGCGTCTTCTATGCTCACTCTCACATTCTGCGTCTCCGCCGCGTCTCGTACTCTCTCGCTAGTCCTACTCACACGTTTTTCACTTGTTTTTTTGCCTAAATTAACGGATTTTGCCACTTTGTCATCGATTTTCAGCGAAAATCTATAGCCGCATCGTGCTTGGCGTATTTCTTTGACTCAGTAGCTTGAAAATCCCGAAAAGTTTCGGAAATTTTGATCCTGGATCCAGGATCCGCGAAAAAGTGGATAAGATTTTGTTTTTTAAGGGTAGCAATTCTAAAATCTAGAGAAAATAGATGGAAAAATTGATTTCCAAGCTATTTCTCACTTAAAAATCAAGAAATTTGCGAAAAGTTTCCAATTTTGCAGAAAAAATTTTGGAATTTGAGCTACATTTTTGATTTCTGGATATTTTTCGCAATTAAAGTATTAAGATTGTCTAATTGGAGAGGTGTGAACGTAGAAATTTGAATTTTGCGAAATTTTACTGCTAATTAGCTTAAGTTTACAAAAAACACCTTATGTACGCCGGGCGCGTTGCGGTCGCGTCGCGGTCTATTTGAAATTTTATGATTTTTCGAGTTTTTAGAAAAAATTAATGCTTTAATTGAAGATTTTAAGCAAAATTAGTCGTGGGAATTATCACAATTTTCTCCTCGCGTTGCGGTCGCGTCGCGGTTTTCTCAAATTTTTTGTTTCTGAAAACCACTATTTCCCTAAAATATTATAGATCTCCAACAAAAGGTACC
3'HR: CTGCAGATGCGCCCAAGAAGGTCCGACTCTACAACAGGTCCCGCCGCGAAATCACTTCCCACCTCTGACGTCGAGGAGGATGAAGCTGCGCCGGATCTCAAGGCAGAATCCGAAGACGTGACGTCATCAGAGGAAATGCAGAACAAGAATCCCGTCAGTGCTCCAGAAAAACGGGCAAAAACCGCCTCATTTAGCCCACCGCCCGCACTTTCGCCCGCCCACTCAATCCAAATGGAAAACGAGGAAATCGAGGTGAAAATGGAGGTGGATAGTGATGAGGAGGAGAAAAAATTACCAAAAATCACTGAAAAACGAGCCAAAATGGTGTATAAGGTGAGACAAGCCTGCACGAGAAACGCGCCAACGTCCAGCTGGTGCATTTCTCGATACCGTACTTCAAAATCCTCGTTTTTTGCAGAAAAAACCCGAAAAAGAGAAAGAAGCAGTGCCATTTATCGATCCAAATGCTCCAAAAAGAAATCGATCTGGATATGTGCATTTTATCATGTAAATTATCGATTTTTCGCGAGAAAATCCCGAAAATCCCCAATTTTTTCAGACATCGCCGAGCTTCATACTCAAAAGCCGTGATGTCACAGAGAGAAATTAATATATCGCTTGCGGCGGACTGGCAGAAGCTTACCGCCGAAGAACGTATCCCGTTTCAGCAGAGAGCCGACGAGGAGAAGGTCAAATATTTGGAGCTGATGGAAGAATACAAGAAAACTGACTCGCATAAGGAATTTCAGAGTTCGTTGTGCTAAAAATCGAGTTCTCCGCAGTTTTTTACCTGTAGGAAGTATAGGAAGCGTTAATTTTGACAACTTTCGGGTTACGGTAGCCCCAAAAGTACGTAAACACCGTACACTCTACGCAAAAATGCACAGAAATTAAAAATTTTGCCAAAACCGTCTACATATGGGTAAAAAGGGTCGCTGAGCTCGATTTTCAACTAGT
KI verification primers: HMG-20-NKIF: 5'-CTACTGTAGCTTTCACTAATTCTCCCC-3' HMG-20-NKIR: 5'-AGCCATTTGACTGAGATGACAGG-3' Download
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Strain name: SYS477 KI background: JIM113 Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; hmg-20 (devKi129 [mNeonGreen::hmg-20]) I
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Expression pattern: Transient-expression
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Lineage expression pattern
350 stage
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600 stage
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3D expression pattern  4-cells  8-cells  15-cells  28-cells  ~50-cells  ~100-cells  ~200-cells  ~350-cells  ~500-cells  ~600-cells  600-cells  bean-stage |
Inferred TF relationships
Based on expression similarity (strong, medium, weak): NA
Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: LIN-13, LSY-2, PHA-4, CEH-82, DPL-1, EFL-1, ELT-3, RNT-1, SWSN-7, TBX-2, UNC-86 |
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 Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: LIN-13, LSY-2, PHA-4, CEH-82, DPL-1, EFL-1, ELT-3, RNT-1, SWSN-7, TBX-2, UNC-86
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