SEL-8

WormBase ID: WBGene00004765

WormBase description: Exhibits transcription coactivator activity. Is involved in Notch signaling pathway; positive regulation of stem cell proliferation; and positive regulation of transcription by RNA polymerase II. Localizes to RNA polymerase II transcription factor complex. Is expressed in several structures, including P3.p hermaphrodite; P4.p hermaphrodite; P5.p hermaphrodite; P8.p hermaphrodite; and neurons.

KI information: mNeonGreen knockin at C-terminus

sgRNA1: ATTGATATTAGCGAATGCAG

sgRNA2: ATAATTGCACAGATATCTTT

5'HR: GCATGCTCAGCTTCAACAGCAGCAAAATAAGATGAGATTGATGCAGCAACAGCAACAGGAAATGCAACGAATTGAACAGCAAAGGAGGCAGCAGATTATGCAGCAACAACAACAGCAGCAGCAACAAGAACATCAACGACAACAGATGCTTCTACAGCAGCAGCAACAACAACAACAAATGCAGCAACATCATCAAATGAATGGAGGTGGACAATTTGCGACGCAGGCACATCAGCAGGCTGCTTATATGCAGCAAATGCAGAGAATGGAGCAGATTCGGTGAGTTTTTTTTCCAAATCGAGAAAAATTCATTAAATTTTCATTGAATTTCTGAAATTTTTCAAAGTTTTTAAATAATCTAATTCGAGAAATCTATCGAAATTTGAAAAAAAATGTATTTAAAATTTATTGATAACAAAGCAAATTACATTTTTTTATGATAAAATTCGGGAGATTTAATTTTTTTTCAAAAATAAAAAATTACAAAAAAAAACCAGTGTAATTCTGACTTAAAAATTTAGATATTGATCATAATCTAGCATAAAAAATTTTTTCAATAGAGCATAGATTAAAATTTTAAAACCCCATAAAAAGTAATTTTAAAAAAATTATCGGATTTTTCTAAAAACGTTTTTTCGATACTCATATTTTAATCAAAAATCAAAAAAAAAAAGGTGAAATTCAACCAATTTTTGGAAAATTCCGAAAAGAAAATTAACTGAAATTTGAACATTTTTGGTAAAAAAAAACCTATAAATGTTTCTGAAATAAGCTCAATTTTCCAGACACCAACAACAACAAGCCCAACAACATCAACAGGCTCAACAGCAACATCAGCAGCAAGCTCAACATCATCAAATGGGTTACGGTATTCCGAATGGATATCCACAGCAAATGCATATGCATCCACCGGCCTACGGAGCCCATCACATGCCACAACCTACTGCCTTCGCTAATATCAATGGTACC

3'HR: CTGCAGTTTTAGAAAATTGCGAAACGAAAAGATATCTGTGCAATTATTCATTTTTTTGTGAATGATTTTTTCCCATTTTTTCCATTTTTCCCCCCGATCAAATTCCTTGCCTTTAAGAGTGGAGTAGCGGTGATTTTGCTTCTTCTAAAAATCTGAAATCGTCTTAAATTTAGAAATTCCACAAAAGAAAAATGAAAAAATACGCGATTTTTCAATTTTGGCTGAATTCAAACTGCAACCAATCAGCGATTCGCTCCGCCCACTTTTGAACCAATAAGATAAATTGGTCTCATTTTGGAGGGAAGTTTAAAAAAATTGAGAAATCACGATTTTTTCGCTTTCTTTGGTGGTTTTTTGTGCAAAATTTATTTTTTTCTCATTCCCACTACTCCACCTCCAATGAGGTTATTCATGTGGTGTAGAAAAATGCAAAAAGTTGTTTTTATACATTTACCCCCCCCCCCCCCCCCCCCCACCTACTCGTGAGTGCCGAAAAATGTTTAAAAACAACGCATCCATTTTTGCATTTTTCGGCTTTTTTTTTGCATTTTTTGACATTTTCTGCATTTTTGGCATTTTTTGGCATTTAGCGCAGAGAGCTGTGTTCACGCAGGATGTCGGAAAAAGCAGAAAATTGTCGAAAATGCCAAAAAAAGTCGAAAAATGTCGAAAAAATGCAAAAAATGAAAGCGTTTCAAAAACATTTTTAGACACTCATGAATAGGGGGAAAATGTATAAAAATACATTTTTGTATTTTTCTACATCATATAAATAGCCCCATAAGCACTGGAATAAGCTTCTTCTAGTCTTCTATCCCCCGCTCTAGTTGTGTATTGTGAAGCGTCTTTTGTCTCATGGCAGGCCATGTCCCATTCCTTGATCATTCCCCTCGATTCTCTCCATCATTTTATGTACCGACTAGT

KI verification primers: SEL-8-CKIF: 5'-AAGCAGTCTCCGATGGAAATGA-3' SEL-8-CKIR: 5'-GTGAGGGACGAAGAGTAGATCAGAA-3'

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Strain name: SYS604

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; sel-8 (devKi176 [sel-8::mNeonGreen]) III

Expression pattern: Ubiquitous

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): MDL-1, ELT-7, PQM-1, MED-2, END-1, ELT-2, NHR-12, RNT-1, NHR-232, GMEB-1, DPFF-1, BAZ-2, CEH-40, T07F8.4, NHR-102


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: ELT-2, NHR-232, ALY-1, CEH-14, CEH-2, CEH-31, CEH-34, CEH-6, CEH-83, CHE-1, CND-1, DMD-4, ETS-7, GMEB-2, HAM-2, HLH-1, HLH-30, HMG-4, LIN-11, LIN-32, M03D4.4, MLS-2, NFYA-1, REF-2, SEM-2, SOX-4, SPTF-1, TBX-2, UNC-39, UNC-86, ZIP-8, RNT-1, BLMP-1, CEH-8, CEH-82, DPL-1, DSC-1, EFL-1, EGL-13, FKH-8, LSY-2, MEP-1, PAX-3, SKN-1, SWSN-7, ZTF-11

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: BLMP-1, PAX-3