M03D4.4

WormBase ID: WBGene00019751

WormBase description: Is an ortholog of several human genes including AC008481.3; ZBTB32 (zinc finger and BTB domain containing 32); and ZFP91-CNTF (ZFP91-CNTF readthrough (NMD candidate)). Is predicted to have nucleic acid binding activity. Is expressed in pharyngeal muscle cell. Human ortholog(s) of this gene are implicated in autosomal dominant non-syndromic intellectual disability 22.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-AATGGATGTTTTCTGCGTCT-3'

Strain name: SYS453

Cross: OP696 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs696 (M03D4.4::TY1::EGFP::3xFLAG + unc-119(+))

Expression pattern: Lineage-specific, Tissue-specific (Pharynx and Muscle)

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): CEH-22, EGL-5, SYD-9, UNC-30, SNAI-1, HLH-8, CEH-2, HLH-1, LET-381, CEH-60, SEM-4, UNC-120, PHA-2, VAB-7, LIN-39, PAX-1, HND-1, CEH-34, EYG-1, CEH-20, DMD-5, HMBX-1, CES-2, Y75B8A.6, UNC-62, TBX-7, F23B12.7, HBL-1, CEH-45, PAL-1, TLP-1, TBX-9, CEBP-1, CEH-40, PHA-4, TTX-1, DVE-1, CEH-51, TBX-2, ATF-7, TBX-8


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: HLH-1, CEH-20, DMD-5, UNC-62, HBL-1, TLP-1, CEBP-1, AST-1, ATHP-1, CEH-13, CEH-38, CEH-39, DAF-19, EGL-18, ETS-5, GMEB-1, GMEB-2, HLH-2, LAG-1, LIM-7, MDL-1, NHR-102, NHR-49, POP-1, PROS-1, SEL-8, SWSN-7, UNC-37, Y55F3AM.14, ZTF-6

Targeted by: HLH-1, UNC-120, CEH-34, UNC-62, PHA-4, NFYA-1, UNC-55, ZIP-8, TBX-2, DSC-1, LSY-2, RNT-1

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: CEH-20, DMD-5, UNC-62, HBL-1, CEBP-1, ATHP-1, CEH-13, CEH-39, DAF-19, ETS-5, GMEB-1, GMEB-2, HLH-2, LAG-1, MDL-1, NHR-49, POP-1, PROS-1, SVH-5, SWSN-7, UNC-37

Targeted by: UNC-120, PHA-4, TBX-2, DSC-1, LSY-2