CEH-22

WormBase ID: WBGene00000445

WormBase description: Is an ortholog of human NKX2-2 (NK2 homeobox 2). Exhibits sequence-specific DNA binding activity. Is involved in cell fate specification; pharyngeal muscle development; and positive regulation of transcription by RNA polymerase II. Localizes to nucleus. Is expressed in several structures, including G1; gonad; neurons; pharyngeal cell; and ventral nerve cord. Human ortholog(s) of this gene are implicated in double outlet right ventricle.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-GAACTTGAACGACGATTCCGC-3'

Strain name: SYS141

Cross: OP389 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs389 (ceh-22::TY1::EGFP::3xFLAG + unc-119(+))

Expression pattern: Pharynx-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): PHA-2, DMD-4, TBX-7, CEH-34, TBX-2, M03D4.4, PHA-4, HMBX-1, ATF-7, Y75B8A.6, CES-2, CEBP-1, F23B12.7, LAG-1


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: LAG-1, AHR-1, NHR-102, SDZ-38, ZTF-6

Targeted by: CEH-34, TBX-2, PHA-4, ETS-4, REF-2, LSY-2

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: LAG-1, AHR-1

Targeted by: CEH-34, TBX-2, PHA-4, ETS-4, REF-2, LSY-2