PHA-2

WormBase ID: WBGene00004011

WormBase description: Is an ortholog of human HHEX (hematopoietically expressed homeobox). Is predicted to have DNA-binding transcription repressor activity, RNA polymerase II-specific and RNA polymerase II regulatory region sequence-specific DNA binding activity. Is involved in several processes, including muscle cell fate specification; pharyngeal gland morphogenesis; and pharyngeal muscle development. Localizes to nucleus. Is expressed in head neurons; intestine; pharynx; and rectal gland cell.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-GCCACGAATTGACACGAATA-3'

Strain name: SYS531

Cross: OP687 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs687 (pha-2::TY1::EGFP::3xFLAG + unc-119(+))

Expression pattern: Anterior-biased, Pharynx-specific

Lineage expression pattern

350 stage


600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): CEH-22, DMD-4, TBX-7, CEH-34, Y48G1C.6, TBX-2, DMD-5, PHA-4, M03D4.4, ATF-7, HMBX-1, CES-2, Y75B8A.6, CEH-36, F23B12.7, CRH-2


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: PHA-4, AST-1, CEBP-1, CEH-27, DAF-19, EGL-18, EGRH-1, ETS-5, GMEB-2, HLH-2, K09A11.1, LIM-7, MDL-1, NHR-102, RCOR-1, SPR-4, TTX-1, UNC-62, ZTF-6

Targeted by: PHA-4, TBX-2, DSC-1, ELT-2

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: PHA-4, CEBP-1, CEH-27, DAF-19, ETS-5, GMEB-2, HLH-2, MDL-1, RCOR-1, TTX-1

Targeted by: PHA-4, TBX-2, DSC-1, ELT-2