CEH-27

WormBase ID: WBGene00000449

WormBase description: Is an ortholog of several human genes including NKX1-2 (NK1 homeobox 2); NKX2-6 (NK2 homeobox 6); and NKX6-3 (NK6 homeobox 3). Is predicted to have DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II proximal promoter sequence-specific DNA binding activity. Is expressed in intestine and nerve ring. Human ortholog(s) of this gene are implicated in several diseases, including congenital heart disease (multiple); congenital nongoitrous hypothyroidism 5; and spastic ataxia 8.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-TGGATGGTGGATGCTATCGG-3'

Strain name: SYS89

Cross: OP135 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs135 (ceh-27::TY1::EGFP::3xFLAG + unc-119(+))

Expression pattern: Lineage-specific, Pharynx-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): CEH-17, LIM-6, CEH-2, EYG-1, DMD-5, CEH-6, CEH-34, PHA-4, CEH-45, PAX-1, ZAG-1, Y48G1C.6, TBX-7, CEH-36, NGN-1, LIM-7, SEM-2, EGL-44


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: PHA-4, NFYA-1, PHA-2, ZIP-8, FKH-10

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: PHA-4, NFYA-1, PHA-2, ZIP-8, FKH-10