CEH-34

WormBase ID: WBGene00000455

WormBase description: Is an ortholog of human SIX1 (SIX homeobox 1) and SIX2 (SIX homeobox 2). Exhibits sequence-specific DNA binding activity. Is involved in mesodermal cell fate specification; positive regulation of apoptotic process involved in development; and regulation of transcription, DNA-templated. Localizes to nucleus. Is expressed in body wall musculature; corpus; head; male sex myoblast; and pharyngeal neurons. Human ortholog(s) of this gene are implicated in branchiootorenal syndrome 2.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-CAAACAAGACATAGAAGCGATAAG-3'

Strain name: SYS173

Cross: OP524 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs524 (ceh-34::TY1::EGFP::3xFLAG + unc-119(+))

Expression pattern: Lineage-specific, Pharynx-specific

Lineage expression pattern

350 stage


600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): PHA-2, CEH-22, CEH-2, PAX-1, DMD-5, TBX-7, DMD-4, EYG-1, UNC-86, CEH-45, Y48G1C.6, PHA-4, M03D4.4, CEH-51, CEH-27, TBX-2, SEM-2, MLS-2, Y75B8A.6, CES-2, CRH-2, CEH-36, IRX-1


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: CEH-22, CEH-2, DMD-5, CEH-45, PHA-4, M03D4.4, TBX-2, CRH-2, AHR-1, ALR-1, AST-1, BCL-11, CAMT-1, CEBP-1, CEH-20, CEH-38, CEH-39, CEH-44, CEH-48, DAF-12, DAF-19, DAF-3, EFL-1, EFL-3, EGL-18, EGRH-1, EOR-1, ETS-5, FOS-1, GMEB-1, GMEB-2, HBL-1, HLH-1, HLH-11, HLH-2, HLH-30, K09A11.1, LAG-1, LIM-7, LIN-13, LIN-15B, LIN-48, LSY-2, MDL-1, MEP-1, NHR-10, NHR-102, NHR-49, PAG-3, RCOR-1, SDZ-38, SEL-8, SKN-1, SOX-2, SWSN-7, SYD-9, UNC-37, UNC-62, Y55F3AM.14, Y73E7A.1, ZAG-1, ZTF-6

Targeted by: PHA-4, CEH-19, HLH-1, UNC-55, UNC-62, ZIP-8, LSY-2

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: CEH-22, CEH-2, DMD-5, PHA-4, TBX-2, CRH-2, ALR-1, AST-1, BCL-11, CAMT-1, CEBP-1, CEH-20, CEH-39, DAF-19, DAF-3, EFL-1, EFL-3, EOR-1, ETS-5, GMEB-1, GMEB-2, HLH-1, HLH-2, HLH-30, K10B3.5, LAG-1, LIN-13, LIN-15B, LSY-2, MDL-1, MEP-1, NHR-10, NHR-49, RCOR-1, SKN-1, SOX-2, SVH-5, SWSN-7, SYD-9, UNC-37, UNC-62, Y73E7A.1

Targeted by: PHA-4, HLH-1, UNC-55, UNC-62, ZIP-8