CEH-51

WormBase ID: WBGene00013583

WormBase description: Is an ortholog of human HOXB8 (homeobox B8). Is predicted to have DNA-binding transcription factor activity and RNA polymerase II proximal promoter sequence-specific DNA binding activity. Is involved in mesodermal cell fate specification; muscle cell fate specification; and pharyngeal muscle development. Localizes to nucleus. Is expressed in several structures, including MSaa; MSap; MSp; MSpa; and MSpp. Human ortholog(s) of this gene are implicated in amelogenesis imperfecta type 4; orofacial cleft 15; and split hand-foot malformation 1 with sensorineural hearing loss.

KI information: mNeonGreen knockin at N-terminus

sgRNA1: ATGAATTGGGGGAATTATAG

sgRNA2: TTAAGATTCGAAGAAGACAA

5'HR: GCATGCATATCTTATTGATCAGATAATTTTTATGTCAGTTGTTTTTTTCTTTTTTGCTTTTTTAAAAGTTTTAAGTAGGCGGGCAATTGTTGTTCGGAAAATTCGGCGAATTCCGGCAAGTTTTTACCCGTTTGCCGGAAATGTTCAATTCCGGCAATGTGCCGGTTTGAAGGAAATTTTCAGTTCCGGCAATTTGCCAGAAATTTTAAATTCCGGCAAATTTTTGCCGGTTTGCAGGAAATTTTCAATTCCGGCAAATTGCCCATTTTGCCAGAAATTTTCAGTTCCGACAATTTGCCGGAAATTTTCAATTCCGCCAATTTGCCGATTTGCCGGAAATTTTCAATTCCGGCAAATCGCCCATTTTGCCAGAAATTTTCAATTCCGGCAGTTTGCCAGTTTGCCGATTTGCCAGAAATTTTTAATTCCGCCGGTTTGCAGGAAATTTCAAATTCCAGCAATTTGTCGATTTGCCAGAAATTTTCAATTCCGGCAACTTGCAAATTTGCCGGAAATTTTTAATTCCGGCAAGTTTTTGCCGAATTGCCGGAACTGCAGTTTTTGACTGAAATTGGCTTAAAAATAAGCTTTTCAGACCAAAATTTCTACCGTAATCCAACACAAAGTACCAAAACTGCCAAATTTTGGCATTGTCCGATTTGTACGAAAAACTACAAGAATCAATCGAAAATTCGCTTTTCCGTGGAAAAACTTCACATTTTTCCAGATTTTCAGACAATTCTGAAGAACAGCCGGAAAATCTCACCCAATTCCCAGGTATCTCACCTCTCCGGTGTCACCTTTTCCCGGTCATGTCTCCCGCCAAACAAAATCGCACCTTCTCGCCTCCCCGCCTACCTAGGCAGCAATGCTCCGCCTAGATTTACAGCCTCCCCAGCTGGAGAATAGCGACTATAATTCCCCCAATTCATTCCACACAAAGGTACC

3'HR: CTGCAGATGTCATCATCCAACTATAATATTAATAACGGCGGTGACTATTATCCTTTGTCCTCCTCAAATCTCAATTCAACTTCTGCTATTACTAATGCTACTGTAATGGAATATTCCACAATGCCAAGCAGCTCTCCGGGCTCAATGAGCTCATCGCCAGCCTATCCGGCGGCCTATCAGGCTCCATCACCGTGCTACGTGGCGGATCCCAATCAGCTTTACTATCAACAACAGCTGGCTCATAATGGGAATGATATGTTGTAAGTTTTTTCGATACTTTTCAGTACAAAACGGGCGTTTTAGAGTGATTTTCACTACGTTTTTCTACCGAAATGCGTGAAGAACCGCTAATTTTAATGGGGTTATTCAATTAGCGTCGGAAAATTAAAAAGTGTAGAAAAATTACGTCACAACTGTATTAAAATACATTTAAAAACATGTATTTAAATACATTTTGCAAGTAACATTTCTTGAATAACCCCATAAGTTTTGACAGTGGGAAATTACTCAAAATATAGCTTTTTCACACTATATGGCCGCGAACGGGCATTTTTAGCAAAAAAAAACTTTGTTAGCTGCTTTGTTGCAATTAACAGCTCTAAAAATTCCAATTTGAACTATTTTGGTAGCTGCAATTTTGCAATTAACAGTGTCGAAAAGTCCAATTTGCACAAATTGGGCAAATTTGGTAGCTGCTTTTTTGCAATTAACAGTGGCCAAAATTCCAAATTTAGGCAATTTTGGTAGCAGCTGCTTTTTTTGCAATTAACAGCTCCAAGAAGTCCAATTTGCACAAATTGGGCAAATTTGGTAGATTTATTGCAACTAGCAATGTCGAAAATTCCAATTTGAACTATTTCGGTAGCTGCTTTTTTGCAATTAAAAGCTCCAAAAATTCCAAATTTATGCAATTTTGGTAGCTGTTTTCCTGCAATTAGCAGTGTCGAAAAACTAGT

KI verification primers: CEH-51-NKIF: 5'-TTGTAGGTTGTAGTTTAGGGAGCAC-3' CEH-51-NKIR: 5'-TGAGCATGGGCTTGAACTCTGTA-3'

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Strain name: SYS397

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; ceh-51 (devKi72 [mNeonGreen::ceh-51]) V

Expression pattern: Lineage-specific, Transient-expression

Lineage expression pattern

350 stage


600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): LET-381, ODD-2, DMD-4, MED-2, TBX-7, HMBX-1, ALR-1, CEH-34, PHA-4, IRX-1, REF-2, M03D4.4


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: NA

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: PAG-3