LET-381

WormBase ID: WBGene00002601

WormBase description: Is an ortholog of human FOXF1 (forkhead box F1). Exhibits sequence-specific DNA binding activity. Is involved in mesodermal cell fate specification; nematode larval development; and positive regulation of mesodermal cell fate specification. Localizes to nucleus. Is expressed in M.dlp; M.drp; body wall muscle cell from M lineage; and coelomocyte. Human ortholog(s) of this gene are implicated in Bamforth-Lazarus syndrome; lens disease (multiple); and persistent fetal circulation syndrome.

KI information: mNeonGreen knockin at N-terminus

sgRNA1: ATATTCTTGGAACTATGGAA

sgRNA2: CTTATAGAAAAATTCCAAGT

5'HR: GCATGCGAAACTTGAATATTTGCATACAAATGTTGTAGTTTCGTGCAATTTAGTACTTCAACTCCATGGTCCAGGTCTGAGTTACAATATTTGTACAGGTATATCACAAGTAGTTACGTAATATCATTACTACTTCCGGTAACGGATGTCGAACATATTTTCCAGTAGTGTTTCTATGACGAATCAATCATATTTTAAAAACTATTTCTTCAGCACCTTTCTGGTATGAGTGATAATCAATGCAATATGGTAGGTACTCACTTTATGCCTTACCTCATTTCTACCTGCTTACACGCGTACCTTTAATCACTATTTCTTATTGTTAGAATCTCTCTCTCTAGATTTCTTAGAATATTAGGTGAACTCAGGTGTTAGTGTTTCACGGATTGCAACTACATTTATAGCAAATTGCTCAGATAGAAATTGAAGAAAAATCAACTGTACTGTAGTGGTAGGCACGGTGCCGCCAGGCAGGCGAATAATCAATTCATCATACTTTTCCCTCATCTTCTCAACCAGATGTATGTCTCTTCAACCACTTTTGCCAACCAAAACTTCTCGCCCATCTCATCACCTCGTATTCTTCCCTAATTTTTCCAAATTGTTTATATTATAGTCATCTCCTCGTCTGTTTTCCATCCATAAGCCACCACCCCATTCTGCTCTCGTTCGTGCTCTTCCTTCCACCACACAGACGTTACCGTACTCCGTGCGAGAGCTATGGGATGGGGCGGAGCTTGCTTGCTTGCGCAAGAGGCCATCAGTTGGGAAATACAAGTTTATTGGGGTCATCTCAATCTCCTAAATACCCTAAGTTTTGTAACTTTTTATTGAGTATACATTATCTCTTTGTCTTGAGTTGTTTTATAATTCCTGAATTTTTCAAAATCCAATGTTACAGACTCATCAACTTCAAATAAAATATTCTTGGAACTGGTACC

3'HR: CTGCAGATGGAATGGTAATAATTTTTGGCATTTTATAACCTTTTACTCTAAAATCTTATAGAAAAATTCCAAGTTCGCGACTGTGCTAAGACATTTCAGCCACTTAATTTCAGTTGATTAAATTTTTTTAAACAGAAATTTTGATACCGATTTCAAACTACCGTATTTTCCCTCTCAGACTTGCATTTCGCCTCTTGCTCTATTTTGACAGTTTATATCTCGGTTGTATTCAGTTTTATTAAAAAAGGTTTAACAACAAAAATATTTTAGACGTAGTTGGTAATTTTTGATAAAACCAAAGACCGAGATATGAACTGTCAAAGTAAAGCAAGGGATGGGCTGCATTTTGCCGCAAACTGTGTGAAATCACAAATCAGATCCGGTTTAAAGCATATTTAACAGACATCTAGAAATGCTATCGTGTTTCTGAAACGACAATGAGGAACTTAAGACTTTTGTTATACTTTTAGTGCATCAAAAGATAAACCATTTCAGCTCAACAGCAATTTATTCTCTTGCCAATGCTCTGGGTCCACTGAAATTGGATAGTACTGCTCCAAACAATTCGCATCGTACAATTAAAGCTGAAAACTACTTTAATGAGGATGAGGAAGATTACAATGAGAACTCGCATGAAGATTCGGAAGATTCAAAAGAAGACAGTGATGGACAAGGATGTAGATCAAGAAAAAGAAAAGAGAAACCACCATTTTCTTATATTGCTTTGATTGCTATGGCTATTTCAGTGAGTTTTCCCTCAAGAACGCATTTTTAAGGTATCACTTAATTTTTCTATATTTTAGTTAGTCATTTTACTGACAACATTTGTTCTTAATTATTTGAATTAAGTAAAATCTCGGATTCAGAAAATCAAAGAAATGATTTATCGTAGTAAGATTGTAAAATCTGAAGTTTGTGCAAAGTTTTAAGGGAAGAGCATCTTAAAAGCCAAACATGTTATGTTTTAAAGTATAAGTATAATTTTAAAAACTGTGTATTCCTTTTGTAAAGACTAGT

KI verification primers: LET-381-NKIF: 5'-ACATTGAACAGGACAGTAGTCACAGG-3' LET-381-NKIR: 5'-GCATTCGTTTAAGCTCAAATTGTG-3'

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Strain name: SYS668

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; let-381 (devKi205 [mNeonGreen::let-381]) I

Expression pattern: Non-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): UNC-30, HLH-8, HND-1, CEH-51, ALR-1, TBX-7, M03D4.4, UNC-120, IRX-1, F23B12.7, CRH-2


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: LSY-2, ZIP-8

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: LSY-2, ZIP-8