HLH-30

WormBase ID: WBGene00020930

WormBase description: Is an ortholog of human MITF (melanocyte inducing transcription factor). Is predicted to have DNA-binding transcription factor activity, RNA polymerase II-specific. Is involved in several processes, including defense response to bacterium; determination of adult lifespan; and regulation of defense response to bacterium, incompatible interaction. Localizes to cytoplasm and nucleus. Is expressed in several structures, including nerve ring; rectal valve cell; somatic cell; spermatheca; and vulva. Human ortholog(s) of this gene are implicated in several diseases, including Tietz syndrome; Waardenburg syndrome type 2A; and ocular albinism with sensorineural deafness.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-GAACGCCTTCTCCGACCTCA-3'

Strain name: SYS82

Cross: OP433 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs433 (hlh-30::TY1::EGFP::3xFLAG + unc-119(+))

Expression pattern: Not expressed in nucleus

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): CEH-10, PAG-3, UNC-39, Y73E7A.1, MBF-1, TAB-1, Y56A3A.28, PROS-1, CEH-41, CEH-8, CEH-32, SEM-2, VAB-3, CEH-31, SOX-2, LIM-7, LIN-32, NHR-67, REF-2, CEH-30, MLS-2, EFL-3, NHR-25, HAM-2, ELT-6, ALR-1, NGN-1, NFI-1, CEH-43, HLH-2, ZAG-1, SPTF-1, ZIP-8


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: PAG-3, LIM-7, CEH-43, HLH-2, ATF-7, BCL-11, CAMT-1, CEBP-1, CEH-20, CEH-38, CEH-39, CEH-44, CES-2, CRH-2, DAF-3, DMD-5, DUXL-1, EFL-1, EGRH-1, ETS-4, ETS-5, FKH-8, FOS-1, GMEB-1, HLH-11, K09A11.1, LIN-13, LIN-15B, LSY-2, MDL-1, MEP-1, MML-1, NHR-10, NHR-102, NHR-35, NHR-49, PHA-4, PQM-1, SEL-8, SKN-1, SWSN-7, TBX-2, UNC-37, UNC-62, ZFH-2, ZTF-6

Targeted by: PROS-1, CEH-31, LIN-32, REF-2, SPTF-1, ZIP-8, ALY-1, BCL-11, BLMP-1, CEH-18, CEH-34, CEH-83, CHE-1, DMD-4, DVE-1, ELT-1, ELT-2, ELT-4, HLH-1, HMG-4, LIN-13, MEP-1, NFYA-1, NHR-232, PHA-4, PQM-1, RNT-1, SYD-9, TBX-2, UNC-120, UNC-42, UNC-55, UNC-62, PAG-3, CEH-8, ZAG-1, AHA-1, ALY-2, C16A3.4, CEH-82, DPL-1, DSC-1, EFL-1, EGL-13, ELT-3, FKH-10, FKH-8, FOS-1, IRX-1, LIN-39, LSY-2, MDL-1, MEF-2, NHR-85, PAX-3, SKN-1, SWSN-7, UNC-130, UNC-86, ZTF-11

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: HLH-2, ATF-7, CAMT-1, CEBP-1, CEH-20, CEH-39, CES-2, CRH-2, DAF-3, DMD-5, DUXL-1, EFL-1, ETS-5, FKH-8, GMEB-1, K10B3.5, LIN-13, LIN-15B, LSY-2, MDL-1, MEP-1, MML-1, NHR-10, NHR-35, NHR-49, PQM-1, SKN-1, SWSN-7, TBX-2, UNC-37, ZFH-2

Targeted by: PROS-1, CEH-31, LIN-32, REF-2, SPTF-1, ALY-1, BCL-11, CEH-14, CEH-18, CEH-34, CHE-1, DMD-4, DVE-1, ELT-1, ELT-2, ELT-4, HLH-1, HMG-4, LIN-13, MEP-1, NFYA-1, NHR-232, PHA-4, PQM-1, RNT-1, SYD-9, TBX-2, UNC-120, UNC-42, UNC-55, UNC-62, PAG-3, CEH-8, ZAG-1, AHA-1, ALY-2, BLMP-1, C16A3.4, CEH-82, DPL-1, DSC-1, EFL-1, EGL-13, ELT-3, FKH-10, FKH-8, FOS-1, HLH-4, IRX-1, LIN-39, LSY-2, MDL-1, MEF-2, NHR-85, PAX-3, SKN-1, SOX-4, SWSN-7, UNC-130, UNC-86, ZTF-11