WormBase ID: WBGene00000469
WormBase description: Is an ortholog of human DBP (D-box binding PAR bZIP transcription factor) and TEF (TEF transcription factor, PAR bZIP family member). Exhibits RNA polymerase II regulatory region DNA binding activity and sequence-specific double-stranded DNA binding activity. Is involved in apoptotic process and positive regulation of programmed cell death. Is expressed in excretory duct cell. Is used to study acute lymphocytic leukemia.
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KI information: mNeonGreen knockin at N-terminus
sgRNA1: CAACCACTGCTCGGCTCACT
sgRNA2: GGACTTTCATAGAGCACTAT
5'HR: GCATGCCCTGTCATTTCTCGAGACAGCCGACGCGCAATCACTCTTATGCTCCGATGGGTATATGGGGCAACAGGGTGGTCCCAGTAGACCCAATTTCTTACAACTTCGTTTTTTTTTAGTTTTTGATTCTACTAATAAAAAATCATATGTAACTTTGAACTACAAAAATCGCGCAAAACTCTTAAGATGTTTCCAGTTACAATACTCTGATAAAACTTAAATTATTCACAAAAAAATATAGATTTCAAATATGACTACTGTAGAGGTCATTTCATTTCCTCCCCATCTAACGTATGCATCTTGAATTTTCTTCAGTGATCATTTTTGATTAAGAAATGTATTCTAGAGCTAAAAACTTGAATTATATTATATACTCTTTTGTAATAACAATTAGCCTACTTCAACTTTTCATTTTAAGATATGACAAGCAAAATTCCAATTATTTTGATTAAACACAACTTTTCAAACTTGTTAAGTAACTTGAGGATCCACATTGACTTTATCAGTACTCACCGCTTATCTAACAACCTCTACCAATTCAGGTTCCACCCTAATCCTAGATTGTCTCTTTCCTCCATTTCCTTTTACTCTTTTTTATGATTGTGCAAACATTTGACCTCTTTCTTTTCTACCTTCCAATATATTCTTCTGTTCAATTTAATTTACCATGGGCATATCTTTGTTTTTTTTTTGAAACGGAATTGGATGGTGTAACCTGAAACATGGCTGGTCTATAGTTGAATTTTCAAGCTTATAGTTTATGAAACTCTAAAAATGTTTTAATCAAAAGTTCAATATTGACGTACCCTATTACATCAATTAAAGGATTTTTTTGGGAATTTTCCATCTAGCCTTTGTCTTCTAGGGTTAAAACTATTCACTTAGCTTATCCATCGGAAGTTTAAACTGAAAATCAAACATTTACCTCTTCAACTCTCTTATAGTTAATAAATCTGTGTCTTTTTTAGCCCCAAATTATCACAGCAGACGCAGGTACC
3'HR: CTGCAGATGGACTTTCATAGAGCCCTATCAGCGCTTTTCACAAATCAAGCAGCCGTTCAGCCACTCCTTGGTTCGCTTGGTTTCCCATTCAACGACGGAACTTCTATTCTGACGACTGCACTTGCTGCACAGTCCGGCGGAAAGAAGTTGGACACTCCGTTGGGGATTTTACCATTTGACTCACTGCCCACGACAAATCTTTTAACACCAACCAAGAAGATCAAACTAGAAGATGAATTGTGTGCCAGTCCAGTGTCAAGCAGGTCGAGTACGGTTAGCAGTTCACACGTGAGTTGGGAAAAATTATTAGCACTTATAAAACTATTGGAATTATTTTCAGTTTTCAAGTCCCCAACGGTCACCATCACGAAAGATGTCGGTTCCGATTCCAGAAGAAAAAAAGGTTTGTTTCAATTTTTTAAGTTTCAAGTGTATACAATTCCAAAAAATCTGAAAACCAATAGAGACTTACCATGAGGTTAAAAATATTCATAAATCGTATTTGCGTGGTCTTAAGTTCAAATCACTACAGTTACGTAACACGACTCCTGTTAGTTACGCAACGACGACCGCGAGGTGTCTGTTGGAGATGGTTTATGGGTGTCAGAATTTTTGATAGTGTGAAAGTCAACCAGTATTAACGCCCCCACCCAAACTATTATAATTATAAGTTGCCTTATTTACCGTATTCCTGGAGTTTCCTCTTAGTGCGGAGCATTGTCAACGCCATTTTGTCTCTAAGCTATGCTAATGAATAATTACTCCTAACAATCCCAATTCAAAAATCCGCTGGTTGTTCCTGGGATAATGCGGTACAGACTGCCTCCAATTAAGAGCTCTTCGAGATTCGGCTCATTGTTTAGCACCACCCAACTGTTGCCATAAGAAAAAGAAGGGGTAATTACATACAGTCTACCTGACAGAGAAACCTTCATGTGTGTGGAGGAATTTGAATATGAATATACGAGAGGACTAGT
KI verification primers: CES-2-NKIF: 5'-GAAAATGCTGCCACCCTCATC-3' CES-2-NKIR: 5'-AAATGAGAATGTGGGTACTTGTACGA-3' CES-2-NKIF2: 5'-TCGTTCTCTTCCCGTGCTCTC-3' CES-2-NKIR2: 5'-CCAAGTCCTCTACGGGGGTA-3' Download
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Strain name: SYS443 KI background: JIM113 Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; ces-2 (devKi112 [mNeonGreen::ces-2]) I
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Expression pattern: Posterior-biased
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Lineage expression pattern
350 stage
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600 stage
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3D expression pattern  4-cells  8-cells  15-cells  28-cells  ~50-cells  ~100-cells  ~200-cells  ~350-cells  ~500-cells  ~600-cells  600-cells  bean-stage |
Inferred TF relationships
Based on expression similarity (strong, medium, weak): SYD-9, EGL-5, CEH-2, SNAI-1, CEH-60, PHA-2, CFI-1, ZFH-2, PAX-1, CEH-31, CEH-22, CEH-5, M03D4.4, CEH-8, PAG-3, PROS-1, UNC-86, HLH-1, TTX-3, ATF-7, AST-1, UNC-120, Y75B8A.6, CEH-48, VAB-7, EYG-1, HBL-1, CEH-34, LIN-39, CEH-6, NHR-67, CEH-30, EGL-46, BAZ-2, CEH-20, TBX-2, CEH-45, LIN-32, SOX-2, DVE-1, TTX-1, DMD-5, EGL-44, CRH-2, Y48G1C.6, CEBP-1
Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: CEH-31, HLH-1, TBX-2, LIN-32, ALY-1, CEH-43, CND-1, HAM-2, HLH-30, HMG-4, MLS-2, REF-2, SPTF-1, TBX-8, UNC-42, UNC-55, ZIP-8 |
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 Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: CEH-31, HLH-1, TBX-2, LIN-32, ALY-1, CEH-43, CND-1, HAM-2, HLH-30, HMG-4, MLS-2, REF-2, SPTF-1, TBX-8, UNC-42, UNC-55, ZIP-8
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