CEH-60

WormBase ID: WBGene00017690

WormBase description: Is an ortholog of several human genes including PBX3 (PBX homeobox 3); PBX4 (PBX homeobox 4); and TGIF2LX (TGFB induced factor homeobox 2 like X-linked). Is predicted to have DNA binding activity. Is expressed in amphid neurons and sensory neurons. Human ortholog(s) of this gene are implicated in holoprosencephaly 4.

KI information: mNeonGreen knockin at N-terminus

sgRNA1: TCTCAAGTGAAAACGTCGAC

sgRNA2: TCACACCGATCTTTGAGAGA CEH-60-NKIsg3: ATTATCCATCTCTCAAAGAT

5'HR: GCATGCTTGTGTGCTGTTGTACTTTCATTGTATTATCTAGTTTTTATACACAAGTTTTGAAACATCAGGTAGATATGCAACAAAAAAACTATCCAAATGCAATACTTAAATTGAGTTTCGGTGATTGGCATGTAATTGAAAAGAAATCGCAACATTTGGGGAATGGTGTTATCAGTGTACATGGTGCACTTGAGCATGGAAGAAAAAACCACTAGAACCGTTCTTTTATCAGTTGAACGTGGTTGTTCAATTATTTTTTCCGTCGCTTTCCATATCGTTTTCTCTTTGAAATTTTCCAATTTTTCCTGATTTCGTAATATATGACAATTAAAATCATCTAATGAGATTAGTGAAGTGTTTGCTATGACACAAATTTGGGTTCGGTTTCTTTTTGTGTAGAAAGGGAAAAGGAAGAGGTGTCACGTAATAAGTCAACATTTATCCTTATGAATTTGAGCGACAGAAAAGGCGGCCCAAATGACAGTAATCCAATAATAATGTTTGCTCTAACAATATCAATAACCCAAATCTACGAGCTACTAGTGGTAGTCTTACTGTACAGTTATTTTCATTTATTGAACACATTTTTGGATTTTTTAATTTTATGTATAAAATTTTTTTGTAACATTTAGTAAAACATAAAATAATAAATAAAACATTTCTATGCATATTAACAAGTAAACTTGACAGGATTTTTTTTTCAAATTCATATCTTAGTAATGATCTATAAAAAATGATGAAATTAAACTCAGTTTTTATCAAATTACACCGATGAATAGAAAAGAAAAAACCTAGACAGAATTCGTACCACCTATTTTAGTTTTTTTTTTTCGTGATTTTTCAAAATTGTATTTAGACACATCACAGCTCTGTGTTGTTGCGTTCACAACCGAGTGCATCACACCGATCTTTGAGAGGGTACC

3'HR: CTGCAGATGGATAATCTTATTAAGCAGCTCCAAATGAGCGTCTCAAGTGAAAACGTTGACAGAAATTTTATTGAGTGAGTTAAAAAAAAAACTTTTGAGCAGCAAAACAGGATTTAAAAATAAATTGACCTCTATGAAAAGAACACTGTTTAATAGATGTTGGCATGACCTATTTTTCAAAAATGTACATCTAAACGGTATTAGATAGGAATAAACTTCTAAGAAAAAAAGCAAAAAAGAAGAAAAATTTAGTTGGGTTTAGGCAAATCTTGATTGTTTGATATTCTATAGTTTGAATGTTTTCATAAGTGATATAAATGACTAATACATATGATCTGCAAAACATCCAGATCAGAGATTCGTCTAGGCATCGTAAGAAGTGTAGTTTGTGATTGAAGAAAATTTATAGAAATGAGAGAACCTGATTCAGATACCGATTTTTATATTTCTTATAAACTGGGTCGGGAGGTGAATCTAAACAGGTCATCTTCCTAGAAAATTTTAGAAAAACATCAGGGAATAGGTGAGACAGTTTTCAGTTCGAGTCATCTTACAAATGATGTGACATATGAATCATAACAGTAGATGCTGATTAAGTTTACACCCCTAGAGAACTATTAGTGAAGTGATGATCATTATTTTTTGTTACCGCTGAAATAGTAAAAACTTATATTAAAAAATTGCAGAAAATGCATCGAAATGAATCCCTGTCGCCGGATGCTTCACGAAGTGCTACTTGAAAGAAAAGACATTTTACGTAAGCAAAGAATGAAAAATCAAAAAGTTATGCAGATTTAGAAAAGGTGAAGGTCACATCCGGTGGTCTTTTACAGAATCAAAAGTTTATGACTATGATATAGCGAAAAGACTCTCTAGTTTAAACAAATTGAAGTTTCAAAAATAATAAAAAAACGGAAGTATGAAATGGATCAGAAATTAACACATTTACATTTCAGGCTCTCGGATGAACTTGGTTGCGTTGTACTAGT

KI verification primers: CEH-60-NKIF: 5'-CGTTGAGTAATAAGCCAAGAGAAAG-3' CEH-60-NKIR: 5'-TCCTTTCAACATAGAATAAGCTTCATC-3'

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Strain name: SYS675

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; ceh-60 (devKi212 [mNeonGreen::ceh-60]) X

Expression pattern: Pharynx-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): DMD-4, UNC-62, M03D4.4, PHA-4, HMBX-1, IRX-1, CES-2


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: ELT-2

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: NA