WormBase ID: WBGene00013543
WormBase description: Is predicted to have DNA binding activity and metal ion binding activity.
|
|
KI information: GFP knockin at N-terminus
sgRNA1: Y75sgRNA1: GGAAATGAACATGATGCATA
sgRNA2: Y75sgRNA1: GGAAATGAACATGATGCATA
Y75sgRNA2: TCAACGTATACTATTTTTGC
5'HR: GCATGCGCGCTTTGCATCGATCCATTGTATACAAATAGAAATAAATATAGGAAGGAAGGAAAAAGATTTGAACTGATCAGGGGAAGTATCAAATGTTTTACAGACCAGACTCTGTGGAATAAATTGAGACTTGATGAAAATGAACGAAAAAATCTGTTTAGGATATAGACAGGGGTGTGCGGCAAATTCGGCAAATTCAGCAAATTCGCCGCACACCCCTGGTACAGTATACCATGTTAAATGTAATCAATTACTTACCACCTTACCGTAGTATAACTGATGATATTTCTGAAGCCCTAACAATTCAAAATAAACGGCTCACTGAAAAAGCAAACCTTGACACTTCCCAGTCTCTCTATCGTTTTACTAAACTAACTGAAGGGGATAAGAATGCAAAAAATAGGCAACAAAAAGAGGTGTCTCTCCCACCTCTTACTATGTGTTGCTGCTGCTGCTCCACCCTTTTTGCTCAAGTCAATGTGTTGTATGAAATATGATTACATTTCAATCTTATTACCTTTTTTGCCCCTTTTTCTCTCCACACAACTCATGTGTACCTATTCACACCCCCCCCCTCTCAAACACGTTTTTCATCTCTTCTTCCAACAACACTTAGGGTTTCTGGAAAAACATTTTCTTCTAGTGACTTTTTTTTTTGAAACTTCATCTCAATATCAGTAGATCACAAAATTCTGAAAATGCGTATGGTGCAACACATTTGACGCGCAAAATATCTCGTAGCGAAAACTACTGTAGCGCTTCTGTCGATTTACGAAGCTCGATTATTGAAAATTTTTGAAATTTCTCAATTTTAGGCTTAAGCTTTTGCTTAGGCTTAGGCTTAGGCTCAGTCTTAGGCTTAGGCTTAGGCTTAGGCTTATACTTAGGCTTAGGCTTATACTTAGGCTTAGGCTTATGCTTAGGCTTAGACTTAATCCTAAGCTAAGGCTTAGGCTTAGGCTTAGACTTAATCCTAGGCTACTGTAGTTTTCGCTGCGAGATATTTTGCGCGTCAAATATGTTGCCAATTACGCATTTTCAGAATTTTGTGTTCCCGTGATATGCTCTATTATTCCCTTTTCGTAATTTTTCAAACAATTTTCAATCAACGTATACTATTTTTGCAGCGGGAAATGAACATGACCGGT
3'HR: CTGCAGATGAATATGATGCACATGGGTGCTCCAATGAACGGGGGCCCGCCCCCACCTCAAATGCTCCAAATGCACCATCCCCTGCCTCACCACGTGGCAGTTTTCGCCCACGGTTCATCGTCACAAAACAGTCAGGCGCCGCCCCTTCAGGCCGCTTCGGCTACACCTCCAGTACCGTCGAGCATGAATCGATCACAAAGgtaagtgtgtggcgggtgtgtaggggggtcacttttttttgggagatggttatcaactttctggcaatcggcaatttcggcaatttcggcacttatcggttttagaaatttccggcaatttcgaaaatatcggcacttgccggttttttgaattttttgtcagtttcgacaatttcatcacttgccggttttggaaattttcggcaacttcggtaatttcggcaattgtcggttttggaaattttcggtaatttcgacaatttcggcacttttgccggttttggaagttatcttcaatcgggaacttcggcacttgttggttttttaagttttcgacaattgccggttttggaaattttcggcaatttcggcacttggcgattttggaaattttcagcaatttttgcaatttcggcaattgcaggttatggaaattttcggcgattggcaatttcggcaattgccagttttggaaattttcggcgatttcggcaattccggcacttgccggttttggaaatttccggcaattggcaatttcggccattgccggttttggcaatttcaggcagttggcaattccggcacttgccggttttgggaatttccggaaagcgacaattccggcacttggcgattttgaaaattttcggaaatttcggtatttccggcaattcccgaagttgccgaaaattcgattgccgcacatacctggtttcaagttcccatatttttcctattagactagcattccgattgctcaagatctgatatctcagtACTAGT
KI verification primers: Y75-KIF: 5'-AGTTTTCGCTGCGAGATATTTTGCG-3' Y75-KIR: 5-TTGACGGCTTGTCTTGAATTTTAGCA-3 Download
|
Strain name: SYS80 KI background: JIM113 Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; Y75B8A.6 (devKi31 [gfp::Y75B8A.6]) III
|
Expression pattern: Non-specific
|
Lineage expression pattern
350 stage
|
600 stage
 |
3D expression pattern  4-cells  8-cells  15-cells  28-cells  ~50-cells  ~100-cells  ~200-cells  ~350-cells  ~500-cells  ~600-cells  600-cells  bean-stage |
Inferred TF relationships
Based on expression similarity (strong, medium, weak): LIN-39, LIN-22, ZFH-2, SYD-9, SNAI-1, RNT-1, PHA-2, PAX-1, EGL-5, CEH-22, CEH-2, NHR-23, LIN-26, BLMP-1, GMEB-1, CEH-16, HAM-2, M03D4.4, CEH-5, NOB-1, VAB-7, HLH-1, CEH-34, BAZ-2, PROS-1, NHR-25, HBL-1, Y48G1C.6, PAG-3, CEH-40, DMD-4, CES-2, CEH-20, VAB-15, UNC-120, CEH-45, AST-1, TBX-7, ATF-7, CEBP-1, PHP-3, TBX-2, F23B12.7, Y56A3A.28, NHR-12, CRH-2, DMD-5, TAB-1
Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: SNAI-1, HAM-2, HLH-1, UNC-120, TBX-2, ALY-1, CEH-36, CEH-83, CND-1, DVE-1, ELT-2, HMG-4, NFYA-1, SPTF-1, UNC-42, UNC-55, UNC-62, BLMP-1, ELT-3, LSY-2, MEP-1 |
|
 Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: SNAI-1, HAM-2, CEH-36, CEH-83, DVE-1, SPTF-1, UNC-42
|