WormBase ID: WBGene00000430
WormBase description: Is an ortholog of human VAX1 (ventral anterior homeobox 1) and VAX2 (ventral anterior homeobox 2). Is predicted to have DNA-binding transcription repressor activity, RNA polymerase II-specific; RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding activity; and RNA polymerase II proximal promoter sequence-specific DNA binding activity. Is expressed in dorsal nerve cord; head muscle; intestine; tail neurons; and ventral nerve cord.
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KI information: mNeonGreen knockin at N-terminus
sgRNA1: AACTCGTATGAACTCTGTAT
sgRNA2: CTCTCCAAATCAAGTTTTGC
5'HR: GCATGCTTATGTCTGTGTTACACCTTAATATATATTCGAAACTATGGGGAACTCTGGGAATAAGAAACACAAGGAAAGGGTTTTTTGAAGAATAAAAATTTCTTATATTACATAGATGTCTGAAGATGAAAATTTTGCGGGAAAAAACAACCAAAATGTGCTACGACACGGGAAATTTTTGGAACAATAGAGATTTTGAATAAATTTACAGAATACCGATTATGAGCACGAACGGATTCCGCAACGGATCACCCAAAATCAAACAGTGGGAAAAGGTTTTAGTTCACTTCCATTAATAAAATTGTCTGGATATATCTAATCTTCCAATTTACAGAAAATCCAATATGTATTAAAAATTCACAACGAAAACCCGGGGACCCGGGGAAAACTATTGATTTATCAAAATTATGTTGATTTTTCGAAGAGAAAAAATATAAATAATTTCTTTTTTTTTAAATTGAACCTTTTCCCCGGAAAAATCAAAAAACAGACACCATTTTTTTGGTACTATAATTTCAAGTTTTAAACTGGTAAGAATAATCACACAGCAGGTGAAAATGCCTTCAGCCTGAAAGCTTTATACTAAATTGGAGTCACGGAATAGTTGAATAAAAAAATATCCTGGGGATATTAGCGATTCACCTATGCACTAGCTTCCTATTGTTTTTAATGCAATTATTTACAGTCTAAGCATCAAATATCTTGCAACTCTCAGTCAAATGAGTCAATTCAAAAGTGTTTTGTTTTGTTTCTATTCAAACTTGGCACTTTCTTATCCTTATTCCAAGAATACAATTAACAATACGTTGGAGTAATTACTGCTGACAGCCTGCTAGTTCCCACCCACTTTCAAGTATCATCCTGTGATGTACCATATGTTGCTCTTCTCAGCTCATTCCATTTATTCACACTTTATTTTCTTGATTCAACTGGTACC
3'HR: CTGCAGATGCCTTCTGCTGATACTGAGTTCATACGAGTTATTCGCATCAAGAGTGCTAATGGTTCAGAGAAGATGCTTGAAATTCCAGCCAAGCTCGACTTGGAAAGACCGAAGAGACCGCGTACTGTATTCACCGATGAGCAATTAGAGGTAAGGCAGAAAATGAAACTGTTGAGATTGTTTAAAAACGATTATCGTTCGAGAAGAAGTTAAAACAAAGCTCGAATTCAGTCACAGCAATCGCTACAGGTTCTTCATTTTACATATTTGTACATCTAATGTAGATCATGAAGTTTGTGACATTTCAAATTTTAAAATGAAGAGATTGCTACAAAATTCATCATAGGTGTTTTTGAGAATCACATTTTTCAGAAGCTAGAAGAATCATTCAACACTTCAGGCTATCTCAGTGGGTCGACTCGAGCAAAACTAGCGGAATCCCTTGGTCTTAGTGACAATCAGGTCAAAGTTTGGTTTCAAAATCGAAGAACCAAGCAGAAGAAAATCGATTCTAGGTGAGTCATTACTCTAATTTAACGGAATATTCTAGGAATTTCAGAGATCCTATAAAGCCGGAAACATTGAAACCTGCTGAAAACTATCAAAACGTCTATCAGAACTATCAAAACTATTGGACAGCCGCTGCATTTCTATCAAACAATGTCATTTCGTCATAATCATTTCGAATTTCAAAGCTACGATAAAAGGACAAAGGAAACTCACCTTTTACTGATAATCTTTCCTTTTTTGTCGCCTCGATTCGGGCTCGTTTGCTTTTCTTCTTGATGTCTGAAAATAATAAATTATAAAAGTGTTTAACATGGAACGAACGTTGGGAGAATTAATCACGATAGCTTGAGAAAAGAAGACAAATATCAAATTCACTGTTAATTTTCATTAATTATCCGAGAAAACATTTTTCGCTGCGGAAACATAATACTAGT
KI verification primers: CEH-5-NKIF: 5'-ACTTTCAGAACCGGAGTTGTTACATA-3' CEH-5-NKIR: 5'-TTAGAAACGTTCAACTCCTAGGCTT-3' Download
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Strain name: SYS424 KI background: JIM113 Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; ceh-5 (devKi103 [mNeonGreen::ceh-5]) I
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Expression pattern: Transient-expression
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Lineage expression pattern
350 stage
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600 stage
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3D expression pattern  4-cells  8-cells  15-cells  28-cells  ~50-cells  ~100-cells  ~200-cells  ~350-cells  ~500-cells  ~600-cells  600-cells  bean-stage |
Inferred TF relationships
Based on expression similarity (strong, medium, weak): PROS-1, TAB-1, Y48G1C.6, NHR-67, SOX-2, Y56A3A.28, CEH-32, HAM-2, NHR-25, EGL-44, CES-2, Y75B8A.6, CEH-43, ELT-6
Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: PHA-4 |
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 Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: CEH-18, CEH-31, MEP-1, NFYA-1, PHA-4, UNC-120, UNC-42, UNC-55, UNC-62, ZIP-8, AHA-1, ALY-2, BLMP-1, C16A3.4, CEH-8, CEH-82, DPL-1, DSC-1, EFL-1, EGL-13, ELT-2, ELT-3, FKH-10, FKH-4, FKH-8, FOS-1, IRX-1, LIN-13, LIN-39, LSY-2, MDL-1, MEF-2, PAG-3, PAX-3, RNT-1, SKN-1, SOX-4, SWSN-7, TBX-2, UNC-130, UNC-86, ZAG-1
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