FKH-8

WormBase ID: WBGene00001440

WormBase description: Is an ortholog of several human genes including FOXD4L4 (forkhead box D4 like 4); FOXE3 (forkhead box E3); and FOXS1 (forkhead box S1). Is predicted to have DNA-binding transcription factor activity, RNA polymerase II-specific. Is expressed in ASEL; ASER; head neurons; neurons; and tail neurons. Human ortholog(s) of this gene are implicated in cataract 34 multiple types; congenital aphakia; and persistent fetal circulation syndrome.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-TTGATACGGATAAGTTGAAAGG-3'

Strain name: SYS496

Cross: OP652 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs652 (fkh-8::TY1::EGFP::3xFLAG + unc-119(+))

Expression pattern: Neuronal-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): NA


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: AST-1, ATF-7, ATFS-1, ATHP-1, CAMT-1, CEBP-1, CEH-23, CEH-36, CEH-38, CEH-39, CEH-43, CEH-44, CEH-48, COG-1, CRH-2, DAF-12, DAF-19, DAF-3, DAF-8, DMD-5, EFL-1, EGL-18, EGRH-1, ETS-5, FKH-10, FOS-1, GMEB-1, HLH-11, HLH-2, HLH-30, HLH-4, K09A11.1, LAG-1, LIM-7, LIN-11, LIN-13, MDL-1, MEF-2, MEP-1, MLS-2, MML-1, NHR-10, NHR-102, NHR-186, NHR-35, NHR-49, NHR-6, POP-1, RCOR-1, SDZ-38, SEL-8, SKN-1, SWSN-7, SYD-9, T18D3.7, TTX-3, UNC-37, UNC-39, VAB-3, Y55F3AM.14, ZFH-2, ZTF-11, ZTF-6

Targeted by: CEH-48, HLH-30, UNC-42

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: AST-1, ATF-7, ATFS-1, ATHP-1, CAMT-1, CEBP-1, CEH-23, CEH-36, CEH-39, CEH-48, CEH-5, CEH-54, COG-1, CRH-2, DAF-19, DAF-3, DAF-8, DMD-5, EFL-1, EGRH-1, ETS-5, GMEB-1, HLH-2, HLH-30, HLH-4, K10B3.5, LAG-1, LIN-13, MDL-1, MEF-2, MEP-1, MLS-2, MML-1, NFYC-1, NHR-10, NHR-186, NHR-35, NHR-49, POP-1, RCOR-1, SKN-1, SVH-5, SWSN-7, SYD-9, T18D3.7, TTX-3, UNC-37, VAB-3

Targeted by: CEH-48, HLH-30, UNC-42