WormBase ID: WBGene00000899
WormBase description: Is an ortholog of human SMAD4 (SMAD family member 4). Exhibits proximal promoter sequence-specific DNA binding activity. Is involved in several processes, including determination of adult lifespan; positive regulation of dauer larval development; and regulation of pharyngeal pumping. Localizes to condensed chromosome; cytoplasm; and nucleus. Is expressed in several structures, including P1; P2; hermaphrodite distal tip cell; hypodermal cell; and neurons. Human ortholog(s) of this gene are implicated in several diseases, including Loeys-Dietz syndrome 3; aortic valve disease 2; and juvenile polyposis syndrome.
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KI information: mNeonGreen knockin at C-terminus
sgRNA1: CTGCTTCGAGCCGCTAGGAA
sgRNA2: GACTAAATCAGTATCTAGTA
5'HR: GCATGCATGAATTATATCTACGAGAAGAAGACTCAGGAAGAGCTGCGAAGGGTAATTTTGAGTGTAAGATTTTGTTTGAAGTTATAAAAATATATTCAGGAAGCAACACGCACCACTGATTCATTGGCCAAGTACTGTTGTGTCCGTGTCTCGTTCTGCAAAGGATTTGGAGAAGCATACCCAGAACGCCCGTCAATTCATGATTGTCCAGTTTGGATTGAGTTGAAAATCAACATGTTAGTTTTTTTTTTTCAAGGCCCTTGTGGTCCCATCAAAATTTTTGAAAGTTGGCTAACTACCTTTTGGGCCCTAGGACCGCCAGAATAATTTCTTAAATTTACGGGTCTTATTATCAAGCTTCGGTACAAGCATAATATCGCGCAGTTTACAATTCATGTATAGATTGAAAAACAATATTTTTTACTCTTGAAACTAAATTTTGTTTCGAATTTCAACATCGACAGCTTATAGTTGCCGTTTGACTCATTTAAAAACTATCATCTGAAAAATATTTGCTAATTGTTCTCTATCGTTTTGAGTTGATTGTTTATGGATATGCCTCTCGCCTCATTTCTGCACTACATCGAGAAGTAAAAGACTTGGTTTTTACAAAAAATAGTGTGAGAGACGATTTTTCAACGCGCAGCAGGCGGAAGCCCGCCGCAAGACGGGCATGGCAGGCGTTCTTGCGCCCTAAAGTAAAATAATCGGGGTACTAAAACTCCTATTACCTGAAATACGGTATGTTAGTAATCTAAAGTAGTTTTATAAAAAATCCCATGTTCATTAAAAAAATCCCAATGTGTAATTTCAGTGCCTACGATTTCATGGATTCAATCTGCCAGTACATAACCAACTGCTTCGAACCTCTGGGTATGGAAGATTTTGCAAAATTGGGAATCAACGTCAGTGATGACGGTACC
3'HR: CTGCAGATGATAACTTTTTTCACTCACGCTACTAGATACTGATTTAGTCTTATTCCAAATCATCCAACGATATCAAACTTTTTCCTTTGAACTTTGCATACTATGTTATCACAAGTTCCAAGCAGTTTCAATACAAACATAGGATATGTTAACAACTTTTGATAAGAATCAAGTTACCAACTGTTCATTGTGAGCTTTGAGCTGTATAGAAGGACAATGTATCCCATACCTCAATCTTTAATAGTCATCAGTCACTGGTCCCGCACCAATTTTTTCGATTCGCATATGTCATATATTGCACCGTGGCCCTTTTTATTGTAACTTTTAATATATTTTCTTCCCAACTTGTGAATATGATTGATGAACCACCATTTTGAGTAATAAATGTATTTTTTGTGGACATCATGTTTAAATAAAGAAAACATAGGCAGTTTGTTAGGAGTTAAGTACACTTTCTGGTAAACTTAATGCCCCTTAAACTTAAATTACAGACAGAGAACTTATAATTTTCAATAGAGAAAAAAACAGACTTTCAGCGATCGGAACTCACACCAGCTACTCAACTACGCCTTTTTTGGTAAATCTTCTACAATAAGAGCCGAAGGTTCAAAAACGTAGTAAGCGTTTATTGAGTGAATAATACAGATATTACACAAAACTTATGACAACTTGCTTACAATTTGTGGAAAACAGTAAACTTCATCGGAGCAGACGCGCGAGACAACTTTTTGACAATGGTTTTTGTGATGTTGCTACCAGATAGCGCCGGTTCCGAATAGAAACGTTTATCTAAATTTTAATTAATTTGATCTTAAATTTAAGTTTACGAGCTTACCGTTGTTAAGAACTCCCAAAGCTTGATTCATCGCTTTCATTTGATGACTGAAGCCACAATCAAACGAGAAAGAGCTCATCTTTTTTGTAGAAGAATTAAGTGGCGGAAAATGATCAATTGGCACACTAGT
KI verification primers: DAF-3-CKIF: 5'-AAGCAAATGGCAACAATGTACTT-3' DAF-3-CKIR: 5'-CTTATGTCCTTATGGCAAGTTACATC-3' Download
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Strain name: SYS542 KI background: JIM113 Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; daf-3 (devKi163 [daf-3::mNeonGreen]) X
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Expression pattern: Non-specific
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Lineage expression pattern
350 stage
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600 stage
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3D expression pattern  4-cells  8-cells  15-cells  28-cells  ~50-cells  ~100-cells  ~200-cells  ~350-cells  ~500-cells  ~600-cells  600-cells  bean-stage |
Inferred TF relationships
Based on expression similarity (strong, medium, weak): Y73E7A.1
Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: CEH-14, CEH-18, CEH-19, CEH-31, CEH-34, CEH-39, CEH-48, COG-1, DVE-1, ELT-2, HAM-2, HLH-1, HLH-30, HMG-4, MEP-1, MLS-2, NFYA-1, PHA-4, PROS-1, REF-2, RNT-1, SPTF-1, TBX-2, UNC-120, UNC-42, UNC-55, UNC-62, ALY-2, BLMP-1, C16A3.4, CEH-8, CEH-82, DPL-1, DSC-1, EFL-1, EGL-13, FKH-10, FKH-8, FOS-1, IRX-1, LIN-13, LSY-2, MDL-1, PAG-3, SKN-1, SWSN-7, UNC-86, ZAG-1 |
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 Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: CEH-14, CEH-19, CEH-31, CEH-34, CEH-39, CEH-48, ELT-2, HLH-1, HLH-30, MLS-2, NFYA-1, TBX-2, UNC-42, UNC-62, ALY-2, CEH-82, DSC-1, EFL-1, EGL-13, FKH-10, FKH-8, FOS-1, IRX-1, LSY-2, MEP-1, RNT-1, SWSN-7, UNC-86, ZAG-1
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