WormBase ID: WBGene00004804
WormBase description: Exhibits Hsp70 protein binding activity and RNA polymerase II regulatory region sequence-specific DNA binding activity. Is involved in several processes, including endoplasmic reticulum unfolded protein response; positive regulation of cellular response to manganese ion; and positive regulation of transcription from RNA polymerase II promoter in response to heat stress. Localizes to endoplasmic reticulum; mitochondrion; and nucleus. Is expressed in several structures, including germ line; head ganglion; intestine; neurons; and pharynx.
|
|
KI information: GFP knockin at C-terminus
sgRNA1: GAGATTTTATGTGATAAAAG
sgRNA2: SKN-1-CKIsg1: TCTTTTATCACATAAAATCT
SKN-1-CKIsg1: GAGATTTTATGTGATAAAAG
5'HR: GTCGACTTAACCTAGAAGCGGCTTAAAATATTTCAAGATATAAGAGAGTTTGAAACCCATACCTTTCAGTTTTCTTATAATTATAAAAATTTCCAGTGTTCCCAACATCCAACTACGCCTACATTGGAATGCAAAACGACAGTCTTCAAGCAGTTGTATCAAATGGACAGATTGACTATGATCATTCCTATCAATCCACTGGTCAGACTCCACTGTCTCCTCTCATCATTGGATCTTCAGGACGTCAACAGCAGACTCAAACGAGCCCAGGAAGCGTCACAGTGACTGCAACAGCTACTCAATCGTTGTTCGATCCATATCACTCACAGAGACACTCGTTTAGTGATTGCACTACGTAAGTTGCTCAGTCTGAAAGTCTAGAAAGTCTCTTGGGATCCCATTCAGCTTATTAGTAAAAGTGCACTTGCTCAAATAGGGCAAGTGGGTACCCATGGGAACAGTTTTCAAAGTACTTTTATGCAAAAGTTCAATAATTACATAATTTCAGTGATTCGTCATCAACGTGCTCTCGCCTCTCTTCGGAATCTCCACGATACACGTCAGAGAGCTCAACCGGAACTCACGAGTCTCGTTTCTACGGAAAGTTGGCTCCATCCAGTGGATCACGCTACCAACGATCATCGTCTCCACGTTCATCACAATCTTCGATTAAGATCGCGAGAGTTGTTCCACTGGCCAGCGGACAACGGAAGCGTGGACGTCAATCCAAGGATGAGCAGCTCGCCAGTGACAACGAGCTTCCAGTGTCGGCGTTCCAGATTTCGGAGATGTCATTAAGCGAGTTGCAACAAGTGTTGAAGAACGAGAGTCTCAGCGAGTATCAAAGACAGTTGATTCGCAAGATTCGTCGACGCGGTAAGCATCATTATTGATGACAGGATCGAAATGATTTTTTTTGTAATTTCAGGAAAGAACAAGGTTGCTGCCCGCACTTGCCGTCAAAGACGCACGGATCGTCACGACAAGATGTCCCATTACATCGTTACC
3'HR: CTGCAGTCGAAACCTTATTAAAGCCACATAATTAAAGATAATTAATTCCGCCACAATAATCGTTTTTTTCTTCTTTGCCGTGTCTCATTTCATTTTGATCTACTCTTTCCTCCCTTCGGATTCTTTGATTTCCCAGTGAAATACCTCACCCACTTCAATCCCCACAAAGTGAGCAACCCCTATCTTGCAACAGTTTTATCATCTCTTCATCATACCCAGTTTGATAATTTATTATCTGATCCCCATCCCCTTGTCGCCTCTCATTAGTATCCTAGTTTTTCATTTGAGCCCGGAGCTCAGACTACATCTCCGAATCATCATACAAATAGATAGAAACGGGTCTCGTGACGAAAGAATACGTGCACCACACGACCCCCCCATCCTGTTCACCCCCATACACCTGAAAAATATGATCTTTACAGTTATTTCTATTATATCCTCAAATCTCTCGTAATATCGTATCAATTTCCTCTTCTTTTTTGTCATTTTCAATTTTTCTCAAATTTCTCAGATCTATTCTTTTTCTTGTATTTTTGGAACTTGTATCCCTCCTCCATCCCCAGACTTCCCCTTCCCAGTTACTCTTGTACATTTTCATATATGTCCATATATCGTTTGAATCTCTCATTTATGGAAATAAATTTGAAAAAATCAATCATTGAAAAGCAAAAATTTCATTCAAATAAAATGATGTTGGTGAATTTAACACAGAAAACAGGGGGACTTGATTTATAAATATAGACAAAACGAGAATTAAGCATCGGCCATCTCAACAGCGGGCTCCGGAGCAGATGGAGAAGGACCAGCCTTCTCGGGTTCAGTTGCCACGGCGATATCCATTGGAGATCCTGCAGAAGCATGGGAACATCCACAGCCACCACCAGCTTGGCGAGCAAACAATAGTTGTTTCACGAGATGAGAGATTCTTCCAGTTTCAAGTTCATCTCCGACTGACTAGT
KI verification primers: SKN-1-CKIR: 5'-CGAGACCCGTTTCTATCTATTTGTAT-3' SKN-1-CKInF: 5'-AGACATTCCAACATTTCCTCCTT-3' Download
|
Strain name: SYS81 KI background: JIM113 Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; skn-1 (hqKi82 [skn-1::gfp]) IV
|
Expression pattern: Transient-expression
|
Lineage expression pattern
350 stage
|
600 stage
 |
3D expression pattern  4-cells  8-cells  15-cells  28-cells  ~50-cells  ~100-cells  ~200-cells  ~350-cells  ~500-cells  ~600-cells  600-cells  bean-stage |
Inferred TF relationships
Based on expression similarity (strong, medium, weak): CEH-44, Y73E7A.1, DPFF-1
Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: BCL-11, BLMP-1, CEH-14, CEH-18, CEH-19, CEH-31, CEH-34, COG-1, DVE-1, ELT-1, ELT-2, ELT-3, HAM-2, HLH-30, NFYA-1, PHA-4, REF-2, RNT-1, SPTF-1, TBX-2, UNC-120, UNC-42, UNC-55, UNC-62 |
|
 Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: BCL-11, BLMP-1, CEH-14, CEH-18, CEH-19, CEH-31, CEH-34, COG-1, DVE-1, ELT-1, ELT-2, ELT-3, HAM-2, HLH-30, NFYA-1, PHA-4, REF-2, RNT-1, SPTF-1, TBX-2, UNC-120, UNC-42, UNC-55, UNC-62
|