TTX-3

WormBase ID: WBGene00006654

WormBase description: Is an ortholog of human LHX9 (LIM homeobox 9). Exhibits RNA polymerase II regulatory region sequence-specific DNA binding activity. Is involved in several processes, including generation of neurons; thermosensory behavior; and thermotaxis. Is expressed in head muscle; head neurons; and pharyngeal neurons. Human ortholog(s) of this gene are implicated in congenital ptosis; hypopituitarism; and myopathy (multiple).

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-ACTTCCGTTTACACCACTTCTCA-3'

Strain name: RW10713

Strain genotype: unc-119 (ed3) III; itIs37 (pie-1p::mCherry::H2B::pie-1 3'UTR + unc-119(+)); stIs10116 (his-72p::his-24::mCherry::let-858 3'UTR + unc-119(+)); stIs10485 (ttx-3::TGF(8G1)::GFP::TY1::3xFLAG)

Expression pattern: Neuronal-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): FKH-10, TAB-1, AST-1, NHR-67, ZAG-1, CEH-48, SOX-2, REF-2, EGL-44, HLH-3, FKH-2, HLH-2, CES-2, ELT-6, ZTF-11, CRH-2, ZIP-8


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: AST-1, DMD-5, ETS-5, K09A11.1, LIM-7, NHR-102, ZFH-2

Targeted by: LIN-32, CEH-8, FKH-8

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: DMD-5, ETS-5

Targeted by: LIN-32, CEH-8, FKH-8