TAB-1

WormBase ID: WBGene00006380

WormBase description: Is an ortholog of human BSX (brain specific homeobox). Is predicted to have DNA-binding transcription factor activity and RNA polymerase II proximal promoter sequence-specific DNA binding activity. Is expressed in head neurons and intestine. Human ortholog(s) of this gene are implicated in amelogenesis imperfecta type 4; orofacial cleft 15; and split hand-foot malformation 1 with sensorineural hearing loss.

KI information: mNeonGreen knockin at N-terminus

sgRNA1: ATCTGAACTTGATTGGGGAG

sgRNA2: TCAATTGATGATCTTCTTCG TAB-1-NKIsg3: CAATTGATGATCTTCTTCGT

5'HR: GGATCCTCACACTTGTTATTGTTATTGTACTGGCTCAATCGCATTGTATTGTAACATAAACACGTAATTAGAGGGAGATGATGAGCATGAGAGGTCGAATTGTGCCAAAAGTGGAAAACGGCTGTAAGTAGGCTTTTCATGTAGGCACAAACACTGCCTGTGGCCTTTGTCTACCACAAAAGTACTGAAAGGTGCGTAGTAGGCATGCCTGAATAAGTACAAAATTTTAAGAATAAGTACAAGACTTACATCGTTAGTGAATTTGAAGAGTTTGCAAAATCAAAATTATTTTGTCAATCAAGTTAATACTTCTAGAGTATTTGGCCAAGAATCTTTTGTAGACCATTTGAAAATTTTCAGAAATTGAGTTGATTGCTAAGCTTTTCCAATTTCTTGATGAGATATTTGAGGTTTGAAAACATTATGTAAATTACGTTTTGTTTTTATTGGAATTTTAATTTCACCTATATGCTAGATATACTATTCCAGATCATCAAAACTGGCAAGAAAAGAAGACTCCAGACCATATACAAAAAATGAACACGTCATCATAATACACGACTGATCGAAAATGACACAACTGATTAGTTTCAATATTTTATTCTAGTACTGAACATTACGAGACAATCAATTTCCATATTTTGCTCATCGTCACTTAAGTGGTTGTTTATTCTTTCATCAACCGCCTACAATATTGCCTACGGCAATACTGTCATTCACAATCATTATCCCTCTCATCAACTCTTTTTTCTTCTTCTCCTTGTCGTCTTTCACGGGACCTTCATCTCTATTCGCCGACTCCGCCCCCTTCCGATGCCGTCTGAAGCTCCACCCATTGTGCTCCTATGGGAGCACCATATTACCGTGTTAGCTCATTTACACGAGAATCTTACCTCCCGTAAAATAATCTTCAAACTTATTTGAAAGGTACC

3'HR: CTGCAGATGATAACTCCTGTATCAAGTTTTTCAATTGACGACCTCCTCCGTGGGCATTCAAACGAAATTCAAAGAATTCTGAAGCTTGATCAAACATCGCCTACTCCTCAATCAAGTTCAGATGAGGTATGTTCTTAAACTATTTTTCTCGTTTGGGTTTTGTAGTCACCATAGGCACTCTGCAAATATTTAAACAAACATATCAGGAATGTATTTCGCTCAATTATCAAAATTGAATTTAAATATAATTTTTCCGTTTTTTTTGGCCTTTGGTCAATGATTTCTTTAAAATTGATTCAAAATAAGTCGCAACTTCATTAAAAAATATTTTGTTTCCTTGATGTGATGCTTGTTCTTTCACAATTACTTAAATTCAACAAGTAATATTTCAATAAAATATGTAAATTTGATCATCAAAAATTACTCTATCGGTTTACTTCCAGGGATCAGAAAAATCATCGATATCCGTAATGTCAGTTCCATTGACAAATGAATTAACTCCCACAATCACTCCTGCTCTTTCTGATCAGCTTTGGCTTGCAGCAGCAGCGTTTCCAATAGAACATAGTCTCTTGTTGGCTCAACGAAATGGAGTTTTTCCTGGATTCTGGTCAACAGCTGATCAGATTCGATTGTCAAATGCAACTAAAGCGTATAGAAGATTGAGGTTGGAATGGTAATGATTGAAATTTATCAAATATAATTATTCTTCTGTTTTCAGTTCGCGAAGAAAAGCAAGAACAGTGTTCAGTGATCAGCAACTACAAGGATTGGAACGAAGATTTGAATCCCAAAGGTGACCAAAAATGCATTCCGAGTTTAGTATTCTAAATCAGACTTTCAGATATCTGAGTACTCCTGAGAGAATTGAATTGGCAAATGCTCTCAATTTGAGTGAAACTCAAGTTAAAACATGGTTTCAAAACCGACGAATGAAGCACAAGAAAGTTGTCAGAAAAGATGATAACTAGT

KI verification primers: TAB-1-NKIF: 5'-AAAGGTTCAAATTGCTCAGTTGC-3' TAB-1-NKIR: 5'-CTTATTCGCTCTCATTATCATTTCCA-3'

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Strain name: SYS674

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; tab-1 (devKi211 [mNeonGreen::tab-1]) II

Expression pattern: Lineage-specific, Neuronal-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): PAG-3, CEH-14, CEH-5, AHA-1, TTX-3, PROS-1, NHR-67, UNC-39, MBF-1, CEH-41, CEH-32, LIM-7, SEM-2, Y48G1C.6, SOX-2, REF-2, HLH-30, TBX-8, Y56A3A.28, NGN-1, ZAG-1, CEH-43, EGL-44, HLH-2, NFI-1, ELT-6, LAG-1, Y75B8A.6


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: NA

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: NA