WormBase ID: WBGene00003592
WormBase description: Is an ortholog of human NFIA (nuclear factor I A); NFIB (nuclear factor I B); and Nfic. Exhibits RNA polymerase II regulatory region sequence-specific DNA binding activity. Is involved in several processes, including determination of adult lifespan; regulation of oviposition; and regulation of pharyngeal pumping. Is expressed in several structures, including body wall musculature; egg-laying apparatus; intestine; neurons; and pharynx. Human ortholog(s) of this gene are implicated in Marshall-Smith syndrome; Sotos syndrome; and chromosome 1p32-p31 deletion syndrome.
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KI information: mNeonGreen knockin at C-terminus
sgRNA1: AGAGCATGTGAAAAGTCAGT
sgRNA2: AAACGAATAAAAAGATTAAC
5'HR: GGATCCAGTTGCAGTGAAACCAATTATGTCAGGATGTAATTCAATCATTCCATCACCCATCACAACTCCTCGTATAACTCCTTCATTTCGAATGCTTGAAGATGATTCATTGATTAATGTACTTGGACAACTTGCACACAGTAATGACGGAACAACTTTAAGTATGATTTTATGAACATTGAACTATCATCCCATTGCTTTTTTTTACAGACGATAGCTTCATTCAACATCTTATTGATACCAATTCTCGATCTCCATTGCTTAGTAGTGGAAATGCTTTTTCGGCATTATCAATGGGAGCTGTGAGTGGATTAGTTCCTGGTAATTCGATTCATCGTCCGGATAGTTCTGCAAGCAAGTAAGTTGAACCATATTGTTTTTTTTCTGAAAATAATGTATCAAAATAAAATGTAATTTTAAAAAAATCTTTCCAGAAATATATACTTTTTTTTAAATTGGCCAAAAGCTTGAAAATTTTATTAATTTCAAATTTCAATCTAAAATCAATGTCTTGTCTAACCAAAAAAAGTTTCTTCAGCGGATCGAATTCGTTGGGTGTAGCAATGGGATTGGCCGTTCCTCAAAATATTGCACTGGCCGTCCAGCAAACTCAAAATGCAATGTCACCTCTTCATCAAGTAAGTTGATATTTTATAAACATTTATAATCAGCAGCTTTTATAATTATTCTGAAAAACACCTTACGAAAATAATTAATCGAAAGATGTAAGCTTTTAATTGTGTAATTCATCGCCAGATTCGTGTTAGCGTTGGAGCTCCACCAGCATGCTCCCCGTCATCATCAAATTCATCTCTAGGAGCTGCAAATCAAGCACCGGTCTCAAATACTCCACAAGATCCGAATGCTCCAAAGCTGCCAACCGATTTCTCCCATGCTCTTCGTAATGAGAAAAAAGGTACC
3'HR: CTGCAGTTTTGATATTCGTGTTAATCTTTTTATTCGTTTTATTTATCCCTTTCGTGTTACCAATTGAAAAATTAGAGTTATCAAACCTAGTCCTTCTTTAGTATTATTCAAACCAGTTGTTATTTTTATTATTTTCATTTTTCTTCAATCTTCAAAACATTTTCTCCGTCTGTGACGTCATAATCTATTTTTCCGAACCTCTTTTGAAAACCAAAATCCACAAAAAAATCAATTGTTTACGGGGCCACACATTTTCCCTTTTCTTTTCTTCACCGTCACCTCTTTATCTTGTAAATCTGAAAAACTTTGAAAAAACGTCGTAGCTATGCACAATTGTAATCACCACTTCCAGTTTCCCAGTGTTTTCCTTTTATGTTTTTTTTGCGTATTTGAACCTCATGTTTGCATTTCTTGTGTGGGTAGTTCAGATTATTTAATTTTTAAAACGTATTTTAATGAATCTTGAATTTTTATCAAGTCGTGAAGCACAACATAGATTCAAAAGAAATTGGAATATCATATCATCAATAAAGAAAAATGATATAGTTAGTTAGTAGCTGAAGAAAAGAAAATAGGTTTATCGGGTGAAACTTGTTTATCAGTCTCCTTCCTGGAAACAAACGAAGATGATAACAACAGCTTACCAGTTAATCAAGTGACATACTTATCCAATTAGATGAATGATAGAGACTGGATAGACAAAAAAGAAGATGTATTGATGGAAAAGATTCCAGTAAGTTCGAAGAGTTGGGTATCTTTTTTACAGATATTATTACAAAATTTAGCAATTGTACAAACACTACCAGGTCTTGGAACGATTGAAACTTTGCAGCTACCGTAACAACGGCAAAACATTGGCAAAACAAAATTTTTTGGCGTTTTCTTAATATTATTCTAGGAATTTTTGGTGTTTTTCTGATTTAAATTTTGGTAACGAGCAAACTAAAGACTAGT
KI verification primers: NFI-1-CKIF: 5'-AACATAATCAACCGAGCACGAG-3' NFI-1-CKIR: 5'-TTAAAATTCATACGCATAGGCTTTT-3' Download
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Strain name: SYS526 KI background: JIM113 Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; nfi-1 (devKi154 [nfi-1::mNeonGreen]) II
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Expression pattern: Lineage-specific, Transient-expression
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Lineage expression pattern
350 stage
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600 stage
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3D expression pattern  4-cells  8-cells  15-cells  28-cells  ~50-cells  ~100-cells  ~200-cells  ~350-cells  ~500-cells  ~600-cells  600-cells  bean-stage |
Inferred TF relationships
Based on expression similarity (strong, medium, weak): RNT-1, LIN-22, NHR-23, CEH-8, PAG-3, LIN-26, GMEB-1, Y73E7A.1, Y56A3A.28, NOB-1, NHR-12, CEH-41, CEH-40, CEH-16, NHR-25, HAM-2, BAZ-2, REF-1, HLH-30, TAB-1, UNC-39, ELT-1, VAB-3, CEH-32
Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: CEH-2, CEH-31, CEH-39, ELT-2, ETS-4, FAX-1, HLH-1, HLH-8, LIM-6, LSY-2, MEP-1, PHA-4, TBX-2, UNC-42, ZIP-8, RNT-1, BLMP-1, C16A3.4, DPL-1, EFL-1, ELT-3, SKN-1, SWSN-7, UNC-130, VAB-15, ZTF-11 |
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 Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: CEH-2, CEH-31, CEH-39, ELT-2, ETS-4, FAX-1, HLH-1, HLH-8, LIM-6, LSY-2, MEP-1, PHA-4, TBX-2, UNC-42, RNT-1, BLMP-1, C16A3.4, DPL-1, EFL-1, ELT-3, SKN-1, SWSN-7, UNC-130, VAB-15, ZTF-11
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