WormBase ID: WBGene00003012
WormBase description: Exhibits RNA polymerase II regulatory region sequence-specific DNA binding activity. Is involved in several processes, including ectoderm development; establishment or maintenance of epithelial cell apical/basal polarity; and vulval development. Localizes to nucleus. Is expressed in several structures, including Z2; epithelial cell; germ line; gonad; and somatic gonad precursor.
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KI information: mNeonGreen knockin at N-terminus
sgRNA1: AATGACACTGGTGGAATACT
sgRNA2: TTAAAAAGTTCGATTAACAT
5'HR: GCATGCTGTTTCAGAATTCGTTTATCTACATCAAAAGTGATCCACAATACAAAGATGAACCACAACAACAATCCAATCAATTCGATGCCACTGTGAACTCTGTTGCTCATGGACAAGCTGGACTTTCAACACTAGAAATGTTCGCTTGTAATGGATCTCCGAACGGTGGTGATGTACTTCGCAGAAAGACGCCGTCTCGGAAAGCTGAAGATTCAATAAAAGCTACGTGTAAAGTATGTGGACATGATGTAATGTATTCTCCTCAAAGGACTTGGAACTTGATGCGTCACGTATGGATTATGCACCAATCATCAAAACCGAATCAGTGTTCAATATGCGGATATTCTCACATCAAGCCATATGTTAGGAAACATATCGAGTCTCAGCATAAGCATGATTCAAATGCAACGATAATTGACTTAAAGTGAGTTTTACATTTCTCAGTTTGATAAAATTGTCAATTTGATTTTCAGATCACCAGAACTAGAAGCTGAATGGAGTCAATTGTTAGATCAATGCTTCGGAGTCACATATCGAAAGTGGAAGCATCATAAGGAGGATACGCAGCACACCGGTGGGTTACTGTTCGATGACGACGAGTTGAGAGGGTGAGCGAGAGCGTCACCGACATCAACTGAAGACCGCCAGTGTAGTCTTTGCAGCGGGTGATGAAGATTACGGTCACGAGCATCATTTTTCACCGGAGGAAGATGTAATGACAACTTTATGATTCTTCATGGCTTTCACTTGTCGACGTCTTCCCATTGTCTTCCATTCATCTTTCGTCATCTCTTCATATTTCACTCAATCACTGCCTTGTGCGATCATGTAATTTCAAACTATTGTAATTCGTTGAAATTATCAAATAATAAATTTAATCAGGTTATTATTGAATGTTTATGTAACCTATTCCATCTCACAATGAGTTAATACAACTCTTGAATACTTGAATACCTTCATCCCTCGCTTACTTTTTTTAAAATTTTTAATTGATTATTTTCAGGGTACC
3'HR: CTGCAGATGCTTTCTAAATTTGTGGTAGTCGAAGTAAGCAACTCAAATAACACAATGACACTCGTCGAGTATTTGGTATGTTTATCATTTAAAAAGTTCGATTAACATTCGTATTTTCAGGTAACGCATGGATTCGACAAGTTTGCAGTTGTAACAGATTTAGTTGATCACCCTTCTTTCAAGTACAAGGATGGTTCATCAAGTCCCGAATCTCCATCAACAACTGCTTCAACTGCTGCCCAACACACACCACCACGAACAGCAGTTTCAACACCTACATCCATCAACACTCCAGTTCCACCACATCAAAACAAACAGAGGCATTCAATTGACAAAATCGCTGCAAATTTATCCACCAAAAAGGTTTCACCATCATCTATTGAGAAGCAGCTCCAAAGAACATCTCATAATCCTCTTCATCAACTATCTACCCCACATGCATTGAGTCAACTACAGAAATTGCTGGAGGAGCAGCACAAGATTAATCAGATGAATATTCAGAAAAAGGAACAGGAGAGGCAGCAAGCTGAGATTCAAAGAATTCTGCTCCAACAGGCAAATGCTGCTCAGATTAATCACTCATTTGGATTAGAAAGGCTAACACCAGAATATGATGATAATCATCATAGTGAAACAATATCTAAAGCTAGCTCTGAAGATTTGAAGACTGAGCCCGATTCAACTGATTTTGGGCTTGGAACAAGCGACGATCAAGTACGTGCTTCAATGCTTCATCTGCTTCATCCAGTATTTGCTCCAGCTTTTGGGATGCTTGATGCCGAGAATATCTTTGGAGCTGCATCAAAACCAACGACGCCTGCATCAAAACGACGAAACACTGATTCAAATGGTGCTCCATCGAAAAAACATCGTTGGCTCCCAGTCAATGAGCTTGAAGAGAGTCGATCATCGCGAGGAAAGAACTGCGGAAGAGTTCATTGCAAGGCCACCTACAAGTGTGCACTCTGTGGAAAGCCAACAACACTCAACTCAACAGGATCTCGTTGGAATCTTCTGCGCCACGTCATCATGATACTAGT
KI verification primers: LIN-26-NKIF: 5'-CACAAACGTAAGTGGTCACTTCTTT-3' LIN-26-NKIR: 5'-ATGAACAACACAATACATGGATAAGG-3' Download
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Strain name: SYS513 KI background: JIM113 Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; lin-26 (devKi150 [mNeonGreen::lin-26]) II
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Expression pattern: Lineage-specific, Skin-specific
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Lineage expression pattern
350 stage
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600 stage
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3D expression pattern  4-cells  8-cells  15-cells  28-cells  ~50-cells  ~100-cells  ~200-cells  ~350-cells  ~500-cells  ~600-cells  600-cells  bean-stage |
Inferred TF relationships
Based on expression similarity (strong, medium, weak): LIN-22, ELT-3, RNT-1, NHR-23, BLMP-1, NHR-35, GMEB-1, NHR-12, C16A3.4, CEH-16, HAM-2, CEH-40, NHR-25, NOB-1, LIN-39, BAZ-2, REF-1, ELT-1, VAB-15, NHR-102, HBL-1, CEH-20, LIN-1, UNC-62, Y56A3A.28, PHP-3, UNC-130, VAB-3, Y75B8A.6, IRX-1, NFI-1, F23B12.7, TLP-1, TBX-2, TBX-8, CEH-13, CEBP-1, PAL-1, CRH-2, SPTF-1, CEH-74, CEH-43, ATF-7
Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: ELT-3 |
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 Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: ELT-3
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