NHR-67

WormBase ID: WBGene00003657

WormBase description: Is an ortholog of human NR2E1 (nuclear receptor subfamily 2 group E member 1). Exhibits DNA-binding transcription factor activity and RNA polymerase II regulatory region sequence-specific DNA binding activity. Is involved in several processes, including gonad morphogenesis; positive regulation of cell cycle arrest; and positive regulation of transcription by RNA polymerase II. Localizes to nucleus. Is expressed in several structures, including excretory cell; head ganglion; neurons; rectal valve cell; and vulval cell. Human ortholog(s) of this gene are implicated in maturity-onset diabetes of the young type 1 and type 2 diabetes mellitus.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-ATGCGACGAGTAGCAATCAA-3'

Strain name: SYS94

Cross: OP373 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs373 (nhr-67::TY1::EGFP::3xFLAG + unc-119(+))

Expression pattern: Lineage-specific, Neuronal-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): CEH-14, CEH-5, TTX-3, DAF-19, TAB-1, COG-1, CEH-41, PHP-3, VAB-7, CEH-48, ZAG-1, HLH-30, CEH-32, SOX-2, HLH-3, CES-2, EGL-44, HLH-2, ELT-6


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: LSY-2, ZIP-8

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: LSY-2