ZFH-2

WormBase ID: WBGene00022518

WormBase description: Is an ortholog of human ZFHX3 (zinc finger homeobox 3). Is predicted to have RNA polymerase II proximal promoter sequence-specific DNA binding activity. Is expressed in anal depressor muscle; anal sphincter muscle; body wall musculature; nerve ring; and vulval muscle. Human ortholog(s) of this gene are implicated in congenital ptosis and prostate cancer.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-TAGATCAACGAAGATCGAGCCG-3'

Strain name: SYS986

Cross: RW12167 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; zfh-2 (st12167 [zfh-2::GFP + loxP + unc-119(+) + loxP] I; unc-119(tm4063) III

Expression pattern: Non-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): FOS-1, ODD-2, DVE-1, CEH-6, TTX-1, PHA-4, CES-2, CEH-36, Y75B8A.6


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: DVE-1, PHA-4, CEH-36, CEH-14, CEH-18, CEH-31, CEH-43, CEH-48, CEH-83, CND-1, COG-1, ELT-2, ELT-4, FKH-10, HAM-2, HLH-30, HMG-4, LIM-6, LSY-2, MEP-1, MLS-2, NFYA-1, PAX-1, PQM-1, SPTF-1, TBX-2, TTX-3, UNC-3, UNC-42, ZTF-11, BLMP-1, CEH-8, DSC-1, FKH-8, SOX-4

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: DVE-1, PHA-4, CEH-36, CEH-14, CEH-18, CEH-31, CEH-83, CND-1, ELT-2, ELT-4, HAM-2, HLH-30, HMG-4, LSY-2, MLS-2, NFYA-1, PAX-1, PQM-1, SPTF-1, UNC-42, DSC-1, FKH-10, SOX-4