DAF-12

WormBase ID: WBGene00000908

WormBase description: Is an ortholog of human NR1I2 (nuclear receptor subfamily 1 group I member 2) and VDR (vitamin D receptor). Exhibits nuclear receptor activity; sequence-specific DNA binding activity; and steroid hormone binding activity. Is involved in several processes, including chemotaxis to cAMP; positive regulation of dauer larval development; and response to biotin. Localizes to nucleus. Is expressed in several structures, including gonad; neurons; pharyngeal muscle cell; somatic gonad precursor; and vulva.

KI information: GFP knockin at C-terminus

sgRNA1: TTTGGTAGATGATTTCTAGT

sgRNA2: ATTGAAGGCATGGCATCGTT

5'HR: GTCGACCCGTATCAAGTTCCACCAGCAGAAAGAAACATCAATTATCAACTGAATGCCGCTGAGCTTAAAGCACTGGATGCTGTCCGAGAAGCTTTCTATGGTATGGACGACCCAATGGAGCAGGGACGTCAAATGCAGTCATTCCTTAAGGTTTCCCAAAAATCCCAGTGAATCTTGTTCATCGTGTTCTTTAGGCAAACAAAACTCCAGCTGATATTATGAATATTATGGATGTTACCATGAGGCGTTTCGTCAAAGTTGCCAAAGGTGTTCCGGCGTTCCGTGAGGTATCTCAGGAAGGAAAGTTTTCGCTTCTCAAGGGGGGAATGATTGAAATGCTGACAGTCCGTGGTGTGACCCGCTACGACGCTTCGACCAACTCTTTCAAGACACCAACAATTAAGGGGCAAAACGTTTCCGTCAACGTGGATGATATGTTTGCTAAATTGAATGCAAATGCACAAGCTCAGAAAGCTAAATGTTTAGAGTACGTTTGCAAAGTTTATCGACAAAAAATCAATAAATCGATTTTAGGTTCTTCGGTTTCTTCGACGAGGAGATTAAAAAGAATGAACTTGCCGTTTATCTAGTCATGTTGGCTGTTCTCTTCTCTGTCCGCTCTGATCCTCCGATGAACGAAAACGATGTTCGCATCGTTACAGAACGACATAATCATTTCATGAGTCTTCTCAACAGGTAATCAATCAACGGATAAAGAAGATATTACAAAACTGGTTTTCAGATATTTGGAATCGTTGTTCGGAGAGCAGGCCCGCCGTATCTTTGAACGGATTCCAAAAGCACTGGGATTACTTAATGTAAGATATCATAAAAGTAGTATTGTCTTTGGGCAACTTAAATAAAATTCAAAACGATAATTTTGAATAATAAATGCACCATAAGAAATCATAAATCATGCTATATAAACACGATAATTTTATGTTTTATTTCAGGAAATTGCTCGTAATGCTGGAATGCTGTTCATGGGAACTGTGCGTTCTGGTGAAGCCGAAGAGCTGCCAGGAGAATTTTTCAAAATCAAAGGTACC

3'HR: CTGCAGGCCATGCCTTCAATACCTCACAACTTGATTCTATATTGCCTCCATCCAACAAACTCAATCTAGCCACATTTCTTCTTTTTCACGTACCTCAACCACCCTTTCCATCCTACAAATCGTATAATATTCCTCTACCTCTTTAACCAATTCATCATCTTTTTATATTGTTTCTTATTTGCATTCAACTTGGAAATAGCCACTATCATATCACTATTGCGTATTTCCTTTCATTTTCTTGTCTTATTTTCTTGAGACCAGCACCAGAAGATTTTTTCGATGGAGAACTAAGCATGATAATTTGAAGTTTTCCATTTAAAAAATGCAGGTAATACGGTTAATTCAATCTGCGAGTTGATGTTCGGTCTCCGGTTTTCATGTTCTACTTCTAATGACTAGAAACCCTTTTATCTAACATCCGGTCCTCCTATCCCTAATGTACCCAGTAGATATTTTTTCCCCGAATGATTAAACCTCCCAGTCAAATATTGTATTTTGTGATTCATGCAGCCTAGCCCCTACCTCTTAATTCCGTCAATACTATCTCAGATTTTCATTGAAGAACTGGTCCGGAATTATTGAATCATCAGCTAGAAAATGGTTTTCCTCAGTCATTTTAAACTTTTTTTCTATTTAAGACTTTCAACTCCCAACGCCTGGCATTTTTTTGTTTCAGATTTCTTTCTTTTTGTGGCCTGTAGTTTACCGATTCATGCGCGCACCCAAAAAATTTCAACAAGTCAATTTCCCGCTAACGTTATATTGTGATGTGTCACTATTTTATTGTGATTATGTGATTGAGAATTTACTGCTTCTACATCTATCCTCTATCCCTGTTCAGTACAATTTTGACTTTCTATCCTTCCTTCTTCAGCACCACCTATTTCTTTTCAACACTGCACTGGTTCATGTTTAGCTACGGTGAATTTATCTTGACAGTAGCTTATGTAACTTTTTCTGTCTGTCTTTTCTCTTTTTTAATATGCAACACCACACCTCTATTTTCCTTGCACTACCTCGATCACACCTCCATTTCTACTAGT

KI verification primers: DAF-12-KIF: 5'-GATGACTCCAACACATGGTTTTCAATA-3' DAF-12-KIR: 5'-CCATCTAATTCAACAAGAATTGGGACAAC-3'

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Strain name: SYS588

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; daf-12 (hqKi213 [daf-12::gfp]) X

Expression pattern: Expressed after bean stage

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): NA


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: ALY-1, BCL-11, BLMP-1, CEH-14, CEH-18, CEH-2, CEH-31, CEH-34, COG-1, DMD-4, ELT-2, ELT-4, ETS-4, HAM-2, MEP-1, NFYA-1, PHA-4, PROS-1, RNT-1, TBX-2, UNC-120, UNC-42, UNC-55, UNC-62, ZIP-8

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: NA