CFI-1

WormBase ID: WBGene00000476

WormBase description: Is an ortholog of human ARID3C (AT-rich interaction domain 3C). Exhibits sequence-specific DNA binding activity. Is involved in cell fate commitment; neuron differentiation; and regulation of transcription by RNA polymerase II. Localizes to nucleus. Is expressed in head muscle; neurons; and pharyngeal muscle cell.

KI information: mNeonGreen knockin at N-terminus

sgRNA1: ACTCAAGAAACTGTTAATGT

sgRNA2: AGTTGATGATCAAACAGAAG

5'HR: GCATGCAATTTCTTCTTAAAAAGTAAAAAAAAAACATTTTAAAAATCTTTAAATAGTCTCGACCCATATCTTGGCCAGTTGTGCGTTTTTGCATTTAACATTTTTTGCACTTTTCGAAATTCACTCAAATAGACTAACAGAAAAAAATTATTTTTTGTACAATTACATATTATCCGTTATTTAAGAGCAATTTGAAAGGATTTTGATGAACAAATACTTTTTTTTTAATCCTACAATGAGAGGCCTCCCAGACGCATCACATTTTTCATTGGCTTTCAAATGAATCATGTATTTTTGTGAATCCGATAGAACTTCAGATTTCAAATAAGAACTCTAATATCCTGACATTTCTCCACTTGAACTTTTATAAACTACTCGCAATTTCGTTCGAATGTTTTTGAGTGATTTGACAATTGATTTCCACCCGTGTCAATTTCGATTAGATCCCCTTCATAAAATTGCACTTCTCTGCCACAATTGCCTTCTCCTCTTGTGCTCTTTTCTCATTCGATGTAAATCATTTCGTCGATTAAAAAAAAACTTCTAAGCAGTTGTTTTCCTTTTCTTCTCGGCATCTTCATCCACCCCTTCCTTGCTAATTGATGCGATATGAAACCATAATGTCTGCCACCCGACCAGTGCAAAAAAAGAACGAGTTCACTTTTGTTCCGGCTCTTCTCCAACACGAACAAACATGTGTTACCCATTAGATTTTTCAAAAAGACCCAAGGAATTTTTGTGGTGTGTGTGGCCCCTCTCTCTAATATCTACAAGAACTTCTCGCTCTATTTCTCGTTCTTTTGATGACCGTGATGATGACGATGGATGATGGAAAATGAATTCTAAGAGTTTGTATTTTCTTGCAGGGTACC

3'HR: CTGCAGATGTCCGTAAGGATTGACGAACCGCAACTGTTCGTCAGTATGTCAAAGGAGCCGACTCAAGAGACCGTCAACGTTGGTGGACATCATGATGATTCAAGCAGTAATTGTGATGAACGAGTTGATGACCAGACTGAGGAGGTTTGGCTTTATTTTTAACTCTTAATACGGTGACCGGTCCGACACGATCAATTATTTTGTTAAATGCAGGAAAGTGTGCGTTTATAAGGAGTACGGTTACTTGAATTTTTCTGACTATAATGTAAATTGATGGCACTTTTTCACTTCTTTATTTTGTTCACTTTTTTATTTTTCAATTTGAACAGTTTTTCAAAGCTTGATAAGTGAGCAAAAAGTATTTTCAATTTACATTAGCAAGATTTTTACGAAGGCATTTTCGGAGCTTCAAAAACCACAAATTCGACATCTTGCTTTCTTTGACTTTTAAAATTTGACGTCTTCACTGAATTTTTAGTGTAGCTCGGATTTTCGGAAAATTTAGTTTCTTCAATATTTATTGGCAAATCTGTGCAGGTCTGAAACTTCTGAAATATCCAAAACTGACAATCCAATAAAAAAATTTCAGCAGAAATCACCTCCAGCTTCTCCCGATCTAACAGCAAACCTAAACGTATTCGACCTGGAATCCCGGCAAAAAGTAGTTCAACGACTTCTAAATTCACAATTGAATCTGTCAAATCTACGAGCGCCTCTCAATTTACCACCGATCTTTCAAGCACTTCAGGGTCCATTCAGTATTCAGCAACAGCTTCTAGGTCTTGCGTCAGGCTTAACAGCAATTTCTCCGGGACTTGACGACTATGACGAAGAGAACACTAATCAAGGAGAACCAGAAGATTTGACATTAGGAGGATTCAGAAAAGAAACATCAGTGAAATCAGAAGAACCATCAGAATCTGGTATAAATGCATCTGGTCCAGCATGGTCATACGAAGAACAGTTTAAGCAGGTTAGCCTTTTTGGGATAAACCATGCTTTATGGGCAAACGATAGGAATAGTTAAAGTTCAATAAAAAAATTGAACTAGT

KI verification primers: CFI-1-NKIF: 5'-ACACTTATTGCATTACAAACCAACC-3' CFI-1-NKIR: 5'-GAACGACTTTATAATGCAAATTTTGA-3'

Download

Strain name: SYS611

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; cfi-1 (devKi183 [mNeonGreen::cfi-1]) I

Expression pattern: Non-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): ZAG-1, CEH-48, SOX-2, HLH-3, EGL-44, CES-2, ZTF-11


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: CEH-31, HLH-1, LSY-2, NFYA-1, PHA-4, TBX-2, UNC-42, UNC-62, BLMP-1

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: CEH-31, PHA-4, LSY-2