NHR-232

WormBase ID: WBGene00012494

WormBase description: Is predicted to have DNA-binding transcription factor activity; sequence-specific DNA binding activity; and zinc ion binding activity.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-TCGTCGCAACTTATCCACCG-3'

Strain name: SYS395

Cross: OP489 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs489 (nhr-232::TY1::EGFP::3xFLAG(92C12) + unc-119(+))

Expression pattern: Lineage-specific, Intestine-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): PQM-1, MML-1, MDL-1, ELT-2, ELT-7, END-1, NHR-102, ODD-2, DVE-1, NHR-12, DPFF-1, PHA-4, ATF-7, CEH-20, HBL-1, F23B12.7, CEH-74, SEL-8


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: NHR-102, HBL-1, SEL-8, CEBP-1, DMD-5, ETS-5, HLH-30, K09A11.1, LIM-7, NHR-23, POP-1, ZTF-6

Targeted by: ELT-2

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: CEBP-1, DMD-5, ETS-5, HLH-30, NHR-23, POP-1

Targeted by: ELT-2