CEH-10

WormBase ID: WBGene00000435

WormBase description: Is an ortholog of human VSX2 (visual system homeobox 2). Is predicted to have DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II regulatory region sequence-specific DNA binding activity. Is involved in generation of neurons; oviposition; and regulation of cell migration. Localizes to nucleus. Is expressed in ABalapppaa; CAN; head neurons; seam cell; and somatic nervous system. Human ortholog(s) of this gene are implicated in several diseases, including WAGR syndrome; diabetes mellitus (multiple); and eye disease (multiple).

KI information: mNeonGreen knockin at N-terminus

sgRNA1: TCATGAGATTCTAGGCATTC

sgRNA2: AATTACTGATTTGTGCACTT

5'HR: GCATGCACAAATGGAATCTATGAAAACTGGGCAGCAACGAAATTTTAAAATTACAGTATTCTTTAAAGGCGCACGCCCTTTAGCAATTCACAAAAAAAGAGTGTCGAAACCTGTTACTGTATTTTTTGCGCAAAACTGCTAGATCTTGCGTATGTGTAAAATGAAAGAACGTATTTTTTCTGAAACTTCGAAAAATTTGGAAAAAAAATGATTTTTTTTACAGACTCATCCTTTTGAAAATAAATTTCATTTCGATTTTTTTTTGAGTTTCAGTTTCAGTTAATTTCTCTGAAATTTACCTCTAATATAAATCTGCTTAAATTAGTCCGTTCAGCATCTCGTTTCCTTGTTAGAACACCTTGATCAATCGGAGTCTCGATGATTTTCAGCACGGCATCTCGATCACATTTCTTGATGAGAAGATAGGTTTCACCTGGAAAAATGGATGGAAATTAGTAATTTCGTTCTGAAAACTTGTAGCAGTTAACTTTTCTGCTGATTTTGCTAATTTGATTTATAAATGGATGTTCTGATAAATTATTAAAAATTCTGGTATTTTCCTGCTCCTTTGAGCCGTGAATTTCTTCAAAAGCGGGTTTTTTCTTCAATCGAATGCTAAATAATTTCTCAAAACACTTCAAATACTCCTTCCTGTTCTTTTTCTCATAATTCTCAGCAATTAGTAACCAGAAGCTCCTCCTCCTTCGTGGTACGGTAGACGTCGATCTGCGAAACCAACCAAAAATGGGCGTGGTTTTCTCAGCGATTCGTCAGGAAATTGGATCATTTGCCTCCTCCCGTTTCTCTAGTGTGCTCTCGACTACTGCACACCGGCCAATTCATTTTTATATCAGCCTCCTCCTTCTCGTCTTCTCATTCCAATTTTCCACTTTGGGTAATCTTCTTCAATTTTTCCAGATAAGCAGCCACTTTTCTCTTGTGGGTACC

3'HR: CTGCAGATGGCGGTGGACGAAATGCAGCTCGCTGCATCAATGCAAATGAGCAATCAAATGAGCAATTCACAACGAATGAGCTTCGCAATTCATGAGATTCTGGGCATTCAAGTAATATCATTCGTATTCGGAAGTGCACATATCAGTAATTTTTAGGGAAATGCATATCTATCTCACGGATATTGTCCTCAGGGAACATTTCTCTCAAATCAACCCAATTTCATGGACATTGGATCCTGTGGAGTATCAGGGTTCGACAGACAGCTGTGTGGTACTGACTCTATTGGAAATGGTAGGATATTCATAGTTCATAGATTCAAACGAATTTTCAATTTTCAGCACCTGCTCCACTGATGTATCGAATGCCAATCTCAACATCAGAGGTTATAACTTCGGAGCCCCAGACTCATGTCAGCTCATCAAGCATCTTATCATCAGCAACTACATCAAATTCTTCTGGTGGTGGAAGCAGTGGTGGTGGTGGAAAGGCAAGCAAGAGAAAGAAGAGAAGACATCGCACAATTTTCACTCAATACCAGATCGACGAACTCGAGAAGGCTTTTCAAGATTCTCATTACCCAGACATCTATGCCAGAGAAGTCCTAGCTGGAAAGACAGAATTGCAAGAAGATCGGATTCAGGTCAGTTGATTTTTCATTGTAGCCCCTTACCAATTACGAAAACGGAGCCCCTTTTATCGGTCGGCATCCATCTTTTTATTGGTGCTAATTTCCTCCATTTTGCTCTTCTTGACTCTCGGAAAGTTAGAGGACATGCTCCCCCACAAGAATAGGGGCAGCAATGTGTCAATTGAATTTCTTGAATTTCCTTCAAGACAAGTCAAGACACGGCCAATTGGCTGATAGTATTGAGTGAAACCCAAAATTCTAAAGGGTTCTCACGCTTCTTCGCTTCTATTGGCTTACTGAAACCTGAGACCTAATTATCTATTAGATGACAAATTGTACTAGT

KI verification primers: CEH-10-NKIF: 5'-TCTTCCGAGTTGTGTGGGACTTA-3' CEH-10-NKIR: 5'-CCATATAGCCCATATTCTGCCATTA-3' CEH-10-NKIF2: 5'-TCTTGTTGTCGTGAGCCCAGT-3' CEH-10-NKIR2: 5'-CACGACGGTTTTGGAACCATAC-3'

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Strain name: SYS427

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; ceh-10 (devKi101 [mNeonGreen::ceh-10]) III

Expression pattern: Neuronal-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): CEH-44, MLS-2, LIN-32, TBX-8, BAZ-2, HLH-30, CEH-32, NGN-1, EFL-3, ETS-7, CEH-43, HLH-2, LAG-1, ZTF-11, ELT-6, ZIP-8


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: NA

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: UNC-42, TBX-2