Single-Cell Protein Expression Atlas of Transcription Factors

WormBase ID: WBGene00012330

WormBase description: Is an ortholog of human ATF5 (activating transcription factor 5). Is predicted to have DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II regulatory region sequence-specific DNA binding activity.

KI information: mNeonGreen knockin at N-terminus

sgRNA1: ACGATGCGGACGACTTCATG

sgRNA2: ACGGCTGCATTTTTGGAGAC

5'HR: GCATGCCAGCCTACCTCATAGGCCTACTTTTTGCCTGCTTGCCTACCTACCAGTATCAAAAGTTCATAGTGCAAGTGTGCTCCACCGCACCGGTGTGTGACCAAAGTGTGCTCTGAAATAGTGTCCTGTTGTTATCGGGAGAGTAGAAGAGTGAGAGCAACACAAAATGCACAGACAAGGCTCGAGAAGTACCTGTCTTCCTACAAATAATTGTTATCAGCACCGTCTCTCTTTTTCTCTCCAACCAATCAAACGGTTTCAGTTTTTTTTTCCTTTTTTTTCGGAGTTTTCCAGCCGCTTTTTATGGTAGTTTTATGATTAGATGAATTGGAATTCATTGTTTTTAATTTTATTGATTTTTTCATTTTGATAATTGTGAAAAAGTAATACGGAGTGTGTAAAAAGTTCAAAAATCCAGTTTAATGAAAAGTAGAATAAAATTTGTTTTTGAAATAGGTTGAGGGTTGTTTAAAAATGTTCAATATTTCCAAAAAGTGTCTCAAAAAGTCATACTAAATTTGTCTAAAACTTTGTAAAATGTTCTATTTTGAAAATGAGAATTTGTTGAAATATCTGTAAAGCAAATAAAAAAAACCAGAAGTGAAAATTTACAATTTTTGATTGTGTCAGCAGTTTTCAAAAGACCTATACTAAATTTTTTTTTTCATTCAATTTTGCTCCGTTAGAAACTTTTTGGTTTCAAACCATTTAGGAGCTACAGAACATTACTTTAAACTGCAAAATGGTACAAACATAAACAAAAAAAAACATAAATAAATTAAACGTTTTTTTATAAAACCAAAAACAGCAGAGATTTAAGCTATCAGATTTGAGTTTTTTTTTTGATTTTTATTTTCTAAATATTATATTTTACAGAAACTAAAAAAACTTCCGCACTGTAAATTTCCATTTTTTCCAGTGAAAAAACCTTCCCTGTCTCCAAAAGGTACC

3'HR: CTGCAGATGCAGCCGTCATACGATGCGGACGATTTCATGCGACGTGGATTTTTCGGCTCTTTTATCGGATTCCGGAAACGATCTTCCAGCAAACCAGGTGAGCTTTATGCAAAAAACGTCACTTATTCTCAAATTTTCTCACGCCATTTGATTGATTGATTGATTTTTTTTTCTGTCAAACCTAACTAATTTATCAATTTTGAAAATGTTAAGTGTTCTTTGCTCCGAGCTCTCCCTATTTGTGGAAAAAATTGCCCATTTCGTGGGCATGGCTTGCTCACCGATTTCTGGCTTCCCTCATAAATTGAAATGGAAGAGTTTAGGCCGAATTAGGCCATTTTGACTCGACCATATCTGGGGTAGATTTACGGCGCTTTGCGTGTCGCGTCGCGGCTAGTTTTTAGTTGTAAAACTAAATGTATTTGTCCGTGTGTGGAGTACACAACTTTTCCACGCGTTGTCCGGCAGGCAATTGTCAATGGAGCGCAAAAAATTCAATGAGGAAGGCCAGACCCCGTGCTTGCTTGCAAAAATCTTAATGGTCCGACAACTGCCGGATTTCGCCCACTGCAAAGTTGTAAATAGTGTGGCGTGGAACCCAGAAATTGTCGGGCTATACATATTGCATTTAAAGGGGATTTTTAGTAAAATTTTGCAAATTTAAGCTCCGCCCCTTTGTTTAAAGCAAAGTTCAAAATGTCACGAAACGTCTCAAAACTTTGCTAAAAACTACAGTACCCTTCCGAAAAATTGCATTATCTATTTGGCATCAGTCTCATACATTCTATTTCTCATCTATCAAATATTTCAATCTTCAATTTCTTCTTTCCTTTCTCTCTCACGTCACATTTTGTTCGCAGGTGGTGGTCGTAGAAGTGACCGACGAGAAGAACTGTTCTTTTTTTTTACTAGT

KI verification primers: ZIP-3-NKIF: 5'-TAGGTACTTGGCAAGTAATATACAGGC-3' ZIP-3-NKIR: 5'-AGCTCTACTAACAGGGGGAAAACTAC-3'

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Strain name: SYS669

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; zip-3 (devKi206 [mNeonGreen::zip-3]) II

Expression pattern: Not expressed

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): NA

Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: ELT-2, PQM-1

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: ELT-2, PQM-1