LIN-31

WormBase ID: WBGene00003017

WormBase description: Is an ortholog of human FOXB2 (forkhead box B2). Exhibits sequence-specific DNA binding activity. Is involved in vulval development. Localizes to nucleus. Is expressed in several structures, including P11.p; P9.p; gonad; nerve ring; and vulval precursor cell.

KI information: mNeonGreen knockin at N-terminus

sgRNA1: AGCCAAATGTACGAGTACGG

sgRNA2: CCCTGAAAAATGCCAAGACC LIN-31-NKIsg3: GTGAGCCAAATGTACGAGTA LIN-31-NKIsg4: CCGGGTCTTGGCATTTTTCA

5'HR: GCATGCGGTATACATTTTTTTGTAGAAAATGTTCACCAAAACTTTTTTCGAATGCATAATAATTCAACATTTTCTATGCTTTTTAAATTATTTTTCTTGTTGCAGAAAACCCAAACTACCGTACTTTTTCACTAGGTGGTCTCCAAACTTTAATCCGTTATAACTCCACAAATATTTTTGATATAAAAAAGTTGTCAATTAACAAAATGTGCAAAATTTTATGACCAATATTTAATCAGTTGACAGTTTTTTGATATCTCAGGTAACCGCAGAGTTATGAGCGTTTGAAGTTGAAGCGTGCTCTAGGCTCTCCAGGTGACCCAAATATTTGAATATTTTAGTAATTCTCATGATTTTAAAATTGTCCAACAAAAATTCAAATATTGACACTAATTCTTCCCACCTAGACACCCCACCATTCCATGGACTAATCCCGTTAATTCGAACGCTTCCCCCCTACCGCTCTCGGTGTCACTCAAAATTTCAAACAAGTCGTGACAGTTCTCGGGTGGTCTGATACCAGTTTACCTACATTTTCAAAAAAAAACACAATCAACTCTTTCATCTTCAGTATTCCAGTGAATCCCGTCCCACACCAGATGCCCCAACTCAGCGGGGGCTGCGTGATACCCGGTCAGCAGCAGCACCAGCAACAGGAGTAACTGGTTCCTCCCAAATATAGGTTAGTGATGTGAATGTAAAACAAAGACTTATTTTTCTATTAGAAATGTGTTGTCATTTTCCTTATTACAAGTTGTTGCCTTTTTCCCTTACGGGCGGTCTCGGTAAGCGCTGTCTCCCCGTTGGCCAGCCCCGCCCACTCAACGCTCCGCCCTTCTTCTCCTTCTAAGGCTTGGCTTCTCAAACAAATTGACTTTTCCCAATCGCTAAGATTTCAATATATTTTGATATATTCCAAAACCTTCTTTATTAATTATCAATCCAAGCAAAACTCTATACATATTCCCTGAAAAGGTACC

3'HR: CTGCAGATGCCAAGACCCGGAAAAGACTCGTACGATGAGCAGAAGCCACCTTATTCATACATCTGGCTCACCTATATGGCTATTCAGGACTCTGACGATAAAATGCTCCCGCTCACGGAGATCTACAAATATATTATGGATCGGTTTCCGTTTTATAGGAAAAACACGCAGAGGTATGAGTTGGGGGTTTGAAAGTTTTTGAGCTCTAAGAAAATTTCTAGTTTTTCTAAAATTCCTGTGTTACTGAAACCCAAAAAAGGGGGGTTTTGTCAATTTTTCCAAGAATCCATTTTTTAAGGCCATTTTGTGAAAAATTTTCAAGTTAAATTTAACAAAAAAATCTGACATTGAAGTTTCCCTTTGTGACTGATTTTGCGTTTTAACAGAGCTTTGAAGATTGAAATGTTTTTTTTCTGAAATTTCGATATTTGTATTTCCATAGTTATTTTGCTTTGAAAAAATATACAAAATATGCTCAAAAACTCCAAATTTCATAGTTTTCAGTCCGGTTTATTTTGGGGTTTTTTCTCACTTTTATAGATTAGTTCTTGTAGTTTCCCCACTTTTATAAGAAAAATTATTGGGTTCAAAAATATGCAATTTTCAATGTTTTAAACAAAAAAAAGTCCTAGAAATATATTTTCGAAGTTTTCTCTTGATTTTTTTATAGTTAAAGTGACTTAAAAATATATGAAATTTCCTTTAAAACAAACAGAAAAATTCTCTAAAAAACTCAAAAAGTGTATTCATTAGTTTCAACATTTCAGAAAAAAAGATAAAAATCAGCTCAAAATGTAATGTTTTCAGGTGGCAAAACTCCCTGCGACACAACTTGTCATTCAACGACTGCTTCATCAAGATCCCAAGAAGAGCCGATCGTCCTGGGAAGGTAATATTTGTAATTTAAAAAAAAATAAAATTTCCGAAGTTATTAATTTAATACTAGT

KI verification primers: LIN-31-NKIF: 5'-AAACATCCTTTCAGAGTTGGATTTT-3' LIN-31-NKIR: 5'-AGCACAAAAAGAGAGCAAACGAG-3'

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Strain name: SYS683

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; lin-31 (devKi220 [mNeonGreen::lin-31]) II

Expression pattern: Expressed after bean stage

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): NA


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: CEH-36, DVE-1, NFYA-1, ZIP-8

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: CEH-36, DVE-1, NFYA-1, ZIP-8